scholarly journals Isolation and Characterization of Guamerin, a New Human Leukocyte Elastase Inhibitor fromHirudo nipponia

1995 ◽  
Vol 270 (23) ◽  
pp. 13879-13884 ◽  
Author(s):  
Hyo Il Jung ◽  
Seung Il Kim ◽  
Kwon-Soo Ha ◽  
Cheol O Joe ◽  
Ke Won Kang
Keyword(s):  
Human Leukocyte ◽  
Leukocyte Elastase ◽  
Elastase Inhibitor ◽  
Isolation And Characterization ◽  
Human Leukocyte Elastase

Preformulation Development of a Human Leukocyte Elastase Inhibitor for Oral Dosage

1996 ◽  
Vol 22 (2) ◽  
pp. 77-83
Author(s):  
Daryl M. Simmons ◽  
Chaoying Zhang ◽  
Robert W. Lee ◽  
Jon R. Swanson
Keyword(s):  
Human Leukocyte ◽  
Oral Dosage ◽  
Leukocyte Elastase ◽  
Elastase Inhibitor ◽  
Human Leukocyte Elastase

scholarly journals Characterization of T4-Binding Globulin Cleaved by Human Leukocyte Elastase

2002 ◽  
Vol 87 (3) ◽  
pp. 1217-1222 ◽  
Author(s):  
Onno E. Janssen ◽  
Henriette M. B. Golcher ◽  
Helmut Grasberger ◽  
Bernhard Saller ◽  
Klaus Mann ◽  
...  
Keyword(s):  
Human Leukocyte ◽  
Leukocyte Elastase ◽  
Human Leukocyte Elastase

scholarly journals Mass spectrometric characterization of nicked fragments of the β-subunit of human chorionic gonadotropin

1997 ◽  
Vol 43 (7) ◽  
pp. 1172-1181 ◽  
Author(s):  
Chuanliang Liu ◽  
Larry D Bowers
Keyword(s):  
Mass Measurement ◽  
Human Leukocyte ◽  
Mass Determination ◽  
Β Subunit ◽  
Leukocyte Elastase ◽  
Human Leukocyte Elastase ◽  
Direct Mass Measurement ◽  
Oligosaccharide Structures

Abstract We describe the use of an HPLC/MS technique for the characterization of nicked fragments of hCG β-subunit. After reductive alkylation of the nicked hCG β-subunit with vinylpyridine, endoproteinase Glu-C or trypsin was used to digest the protein to produce peptides that could be analyzed by HPLC/electrospray ionization MS. Human leukocyte elastase digestion was used to produce an experimentally nicked hCG. Two nicking sites were observed, between amino acids 42Thr and 43Arg and between 44Val and45Leu. The former site has not been previously reported for elastase digestion. The structures of the fragments were confirmed by HPLC/MS after removal of the oligosaccharide by direct mass measurement and by mass determination of their proteolytic digests. Without the glycopeptidase treatment, the microheterogeneity of the two N-linked oligosaccharides could be deduced from the spectra of the proteolytic fragments. Nicking with elastase was found to alter the oligosaccharide structures. Nicked β-subunit samples isolated from the urine of choriocarcinoma patients were also analyzed and the location of the nicking site(s) agreed with that determined by classical techniques. Important differences in the oligosaccharide structures were also observed in these samples, including the presence of triantennary oligosaccharides not found in hCG from healthy subjects. These findings demonstrate the potential of HPLC/MS for characterization of glycoprotein standard preparations.

ChemInform Abstract: An Asymmetric Synthesis of L-694,458, a Human Leukocyte Elastase Inhibitor, via Novel Enzyme Resolution of β-Lactam Esters.

ChemInform ◽  
2010 ◽  
Vol 28 (6) ◽  
pp. no-no
Author(s):  
R. J. CVETOVICH ◽  
M. CHARTRAIN ◽  
F. W. JUN. HARTNER ◽  
C. ROBERGE ◽  
J. S. AMATO ◽  
...  
Keyword(s):  
Asymmetric Synthesis ◽  
Human Leukocyte ◽  
Leukocyte Elastase ◽  
Elastase Inhibitor ◽  
Human Leukocyte Elastase
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