scholarly journals Human Transferrin Confers Serum Resistance againstBacillus anthracis

2010 ◽  
Vol 285 (36) ◽  
pp. 27609-27613 ◽  
Author(s):  
Suzan H. M. Rooijakkers ◽  
Suzanne L. Rasmussen ◽  
Shauna M. McGillivray ◽  
Thomas B. Bartnikas ◽  
Anne B. Mason ◽  
...  
1994 ◽  
Vol 269 (39) ◽  
pp. 24504-24510
Author(s):  
A. Espinosa de los Monteros ◽  
B.E. Sawaya ◽  
F. Guillou ◽  
M.M. Zakin ◽  
J. de Vellis ◽  
...  

2013 ◽  
Vol 162 (1) ◽  
pp. 278-284 ◽  
Author(s):  
Yi Zou ◽  
Saixiang Feng ◽  
Chenggang Xu ◽  
Bin Zhang ◽  
Suming Zhou ◽  
...  

2006 ◽  
Vol 74 (7) ◽  
pp. 3742-3755 ◽  
Author(s):  
Lakshmi Pillai ◽  
Jian Sha ◽  
Tatiana E. Erova ◽  
Amin A. Fadl ◽  
Bijay K. Khajanchi ◽  
...  

ABSTRACT Human diseases caused by species of Aeromonas have been classified into two major groups: septicemia and gastroenteritis. In this study, we reported the molecular and functional characterization of a new virulence factor, ToxR-regulated lipoprotein, or TagA, from a diarrheal isolate, SSU, of Aeromonas hydrophila. The tagA gene of A. hydrophila exhibited 60% identity with that of a recently identified stcE gene from Escherichia coli O157:H7, which encoded a protein (StcE) that provided serum resistance to the bacterium and prevented erythrocyte lysis by controlling classical pathway of complement activation by cleaving the complement C1-esterase inhibitor (C1-INH). We purified A. hydrophila TagA as a histidine-tagged fusion protein (rTagA) from E. coli DE3 strain using a T7 promoter-based pET30 expression vector and nickel affinity column chromatography. rTagA cleaved C1-INH in a time-dependent manner. The tagA isogenic mutant of A. hydrophila, unlike its corresponding wild-type (WT) or the complemented strain, was unable to cleave C1-INH, which is required to potentiate the C1-INH-mediated lysis of host and bacterial cells. We indeed demonstrated colocalization of C1-INH and TagA on the bacterial surface by confocal fluorescence microscopy, which ultimately resulted in increased serum resistance of the WT bacterium. Likewise, we delineated the role of TagA in contributing to the enhanced ability of C1-INH to inhibit the classical complement-mediated lysis of erythrocytes. Importantly, we provided evidence that the tagA mutant was significantly less virulent in a mouse model of infection (60%) than the WT bacterium at two 50% lethal doses, which resulted in 100% mortality within 48 h. Taken together, our data provided new information on the role of TagA as a virulence factor in bacterial pathogenesis. This is the first report of TagA characterization from any species of Aeromonas.


Structure ◽  
1998 ◽  
Vol 6 (10) ◽  
pp. 1235-1243 ◽  
Author(s):  
Hendrik Fuchs ◽  
Uwe Lücken ◽  
Rudolf Tauber ◽  
Andreas Engel ◽  
Reinhard Geßner

1994 ◽  
Vol 3 (9) ◽  
pp. 1476-1484 ◽  
Author(s):  
Maurizio Orlandini ◽  
Annalisa Santucci ◽  
Anna Tramontano ◽  
Paolo Neri ◽  
Salvatore Oliviero

Biochemistry ◽  
1991 ◽  
Vol 30 (45) ◽  
pp. 10824-10829 ◽  
Author(s):  
Robert C. Woodworth ◽  
Anne B. Mason ◽  
Walter D. Funk ◽  
Ross T. A. MacGillivray

Sign in / Sign up

Export Citation Format

Share Document