scholarly journals Protein-degradation products and bacterial enzyme activities in faeces of breast-fed and formula-fed infants

2003 ◽  
Vol 89 (4) ◽  
pp. 509-515 ◽  
Author(s):  
Patricia M. Heavey ◽  
Shirley-Anne H. Savage ◽  
Alison Parrett ◽  
Cinzia Cecchini ◽  
Christine A. Edwards ◽  
...  
Keyword(s):  
Enzyme Activities ◽  
Degradation Products ◽  
Milk Formula ◽  
Formula Milk ◽  
Functional Changes ◽  
Glucuronidase Activity ◽  
Bacterial Enzyme ◽  
Faecal Samples ◽  
Significant Difference ◽  
Breast Fed

The aim of the present study was to determine the effects of age and diet (breast milk, formula milk and weaning diet) on metabolic activities in faecal samples from infants aged 1 week to 1 year, and to compare these findings with activities found in samples from adults. Such activities can provide valuable information on functional changes in the microbiota that may have significance for the health of the host. Fresh faecal samples were collected from forty-four breast-fed infants (twenty-four males, twenty females) and thirteen formula-fed infants (three males, ten females) throughout the first year of life. The samples were analysed for protein-breakdown products, including the faecal concentrations of NH3, phenol and p-cresol, and faecal bacterial enzyme activities. There was wide individual variation in all variables measured; however, the values in infants were substantially lower then those found in adults. In pre-weaned infants, faecal NH3 concentration and β-glucuronidase activity were the only endpoints that were significantly different in breast-fed and formula-fed infants (P<0·001 and P<0·05 respectively). This was not apparent after weaning. There was a significant difference between the breast-fed and formula-fed weaned groups and their pre-weaned counterparts only for NH3 (P<0·05). β-Glucuronidase activity and phenol concentration were significantly (P<0·01) greater in weaned breast-fed infants compared with pre-weaned breast-fed infants. No differences were observed between pre-weaned and weaned formula-fed infants for any of the variables except for NH3 concentration. It can be concluded from the present study that there are significant differences in two faecal characteristics between breast- and formula-fed infants and that changes occur as the infants grow older and are weaned onto solid foods.

scholarly journals Steatocrit Value in New-Born Babies

2018 ◽  
Vol 35 (7-8) ◽  
pp. 190-3
Author(s):  
Johnwan Usman ◽  
Irfan Abdullah ◽  
Muhazar Muhazar ◽  
Atan Baas Sinuhaji ◽  
A. H. Sutanto
Keyword(s):  
Body Weight ◽  
Breast Milk ◽  
Prospective Study ◽  
The Body ◽  
Milk Formula ◽  
Preterm Newborn ◽  
Formula Milk ◽  
Diet Patterns ◽  
Significant Difference ◽  
A Prospective Study

A prospective study on steatocrit value in full-term or preterm newborn babies of~ 2 days of age in Dr. Pimgadi Hospital, Medan is reported. This study was conducted from December 7, 1992, to February 7, 1993; there were 72 newborn babies (37 males and 35 females). The body weight was> 2500 gin 60 babies and s. 2500 gin 12 babies. The median steatocrit value in babies with body weight of> 2500 g was 9%, and it was 32% in babies less than 2500 g of body weight. The proportion of babies with > 25% steatocrit value was larger in babies less than 2500 g than that in 2500 g by the diet patterns of breast milk, breast milk and milk formula, milk formula. The proportion of babies with < 25% steatocrit value was higher in babies with body weight of< 2500 g than that in babies > 2500 g for those who had either breast milk, breast milk and milk formula or milk formula. There was significant difference (p<0,05) in the steatocrit levels between babies with the body weight of > 2500 g and those who had body weight of ≤ 2500 g.

scholarly journals Effect of Lactobacillus plantarum LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

2016 ◽  
Vol 66 (3) ◽  
pp. 294-303 ◽  
Author(s):  
Emília Hijová ◽  
Jozef Kuzma ◽  
Ladislav Strojný ◽  
Alojz Bomba ◽  
Izabela Bertková ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Enzyme Activities ◽  
Probiotic Strain ◽  
Coliform Bacteria ◽  
Control Group ◽  
Sprague Dawley ◽  
Positive Effects ◽  
Glucuronidase Activity ◽  
Bacterial Enzyme ◽  
Prevention Of Cancer

Abstract The effect of the probiotic strain Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities – β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in the prevention of cancer, atherosclerosis and dysbiosis was investigated. Male Sprague-Dawley rats were randomly divided into 12 experimental groups: C (control group), AT (atherosclerotic group), CC (carcinogenic group), and then each group in combination with antibiotics and probiotics individually and each group in double combination on antibiotic and probiotic. In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in the atherosclerotic group significantly increased the activity of β-glucuronidase (p<0.001) and β-glucosidase (p<0.01). Azoxymethane application in the carcinogenic group significantly increased β-glucuronidase (p<0.01), but reduced β-glucosidase (p<0.01). Daily application of probiotics individually and in double combination with antibiotics increased the activity of β-galactosidase, and β-glucosidase, and positively decreased the level of β-glucuronidase. In the control antibiotic group β-glucuronidase was significantly increased (p<0.05), and β-glucosidase decreased (p<0.01) which can be caused by a change of microflora in favor of coliform bacteria. These finding indicate the positive effects of probiotic Lactobacillus plantarum LS/07 which allows its use in disease prevention in human and veterinary medicine.

scholarly journals Cariogenic potential of cows', human and infant formula milks and effect of fluoride supplementation

2008 ◽  
Vol 101 (3) ◽  
pp. 376-382 ◽  
Author(s):  
Regina Celia Rocha Peres ◽  
Luciane Cristina Coppi ◽  
Maria Cristina Volpato ◽  
Francisco Carlos Groppo ◽  
Jaime Aparecido Cury ◽  
...  
Keyword(s):  
Human Milk ◽  
Infant Formula ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Milk Formula ◽  
Streptococcus Sobrinus ◽  
Formula Milk ◽  
Significant Difference

The aim of the present study was to evaluate the cariogenicity of cows', human and infant formula milks, supplemented or not with fluoride, in rats. Sixty female Wistar rats were desalivated and infected withStreptococcus sobrinus6715.Animals were divided into six groups: group 1, sterilised deionised distilled water (SDW; negative control); group 2, 5 % sucrose added to SDW (positive control); group 3, human milk; group 4, cows' milk; group 5, Ninho®formula reconstituted with SDW; group 6, Ninho®formula reconstituted with 10 parts per million F and SDW. At day 21 the animals were killed and their jaws removed to quantify total cultivable microbiota,Strep. sobrinusand dental caries. The concentration of carbohydrate and fluoride in the milks was analysed. The Kruskal–Wallis test (α = 5 %) was used to analyse the data. The caries score by the milk formula was as high as that provoked by sucrose. Regarding smooth-surface caries, human milk was statistically more cariogenic than cows' milk, which did not differ from the SDW and the Ninho®with fluoride (P>0·05). Groups 2–6 showed higherStrep. sobrinuscounts when compared with the negative control group (P < 0·05) but no statistically significant difference was found among them (P>0·05). HPLC analysis showed that infant formula had 9·3 % sucrose and 3·6 % reducing sugars. The infant formula should be considered cariogenic due to the sugars found in it, but fluoride supplementation reduced its cariogenic effect. The human milk was more cariogenic than the cows' milk but not as much as the formula milk.

scholarly journals Determination of fluoride concentration in powdered milk in Iran 2010

2011 ◽  
Vol 107 (7) ◽  
pp. 1077-1079 ◽  
Author(s):  
Amir Hossein Mahvi ◽  
Maryam Ghanbarian ◽  
Marjan Ghanbarian ◽  
Ahmad Khosravi ◽  
Masoud Ghanbarian
Keyword(s):  
Dental Fluorosis ◽  
Fluoride Concentration ◽  
The Body ◽  
Milk Formula ◽  
Powdered Milk ◽  
Formula Milk ◽  
Adverse Health Effects ◽  
Three Samples ◽  
Significant Difference ◽  
High Concentrations

High concentrations of fluoride (F) in powdered milk (formula milk) can have adverse health effects on the body. The F concentration in powdered milk was analysed in Iran in 2010. A total of twelve commercial brands of highly consumed powdered milk were selected to analyse the F content through the standard F ion-selective electrode method. From each brand, three samples with different production dates were selected. The means and standard deviation for F concentration in all the samples was 1·73 (sd 0·3) μg F/g. The minimum and maximum F content in powdered milk brands Humana2 and Humana3 was 1·32 (sd 0·1) and 2·36 (sd 0·3) μg F/g, respectively. The study revealed that there was no significant difference in F concentration in the samples that belonged to various dates. Humana3 had a high F concentration (with an average of 2·36 (sd 0·3) μg F/g), which can be a risk factor for increased dental fluorosis, especially when being prepared using water with a high content of F.

Immunoreactivity of Tissue Plasminogen Activator and of Its Inhibitor Complexes

1989 ◽  
Vol 61 (03) ◽  
pp. 409-414 ◽  
Author(s):  
M Rånby ◽  
G Nguyen ◽  
P Y Scarabin ◽  
M Samama
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Degradation Products ◽  
Gel Filtration ◽  
Free Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Significant Difference ◽  
Tissue Plasminogen ◽  
Pai 1

SummaryAn enzyme linked immunosorbent assay (ELISA) based on goat polyclonal antibodies against human tissue plasminogen activator (tPA) was evaluated. The relative immunoreactivity of tPA in free form and tPA in complex with inhibitors was estimated by ELISA and found to be 100, 74, 94, 92 and 8l% for free tPA and tPA in complex with PAI-1, PAI-2, α2-antiplasmin and C1-inhibitor, respectively. Addition of tPA to PAI-1 rich plasma resulted in rapid and total loss of tPA activity without detectable loss of ELISA response, indicating an immunoreactivity of tPA in tPA/PAI-1 complex of about l00%. Three different treatments of citrated plasma samples (acidification/reneutralization, addition of 5 mM EDTA or of 0.5 M lysine) prior to determination by ELISA all resulted in increased tPA levels. The fact that the increase was equally large in all three cases along with good analytical recovery of tPA added to plasffi, supported the notion that all tPA antigen present in plasma samples is measured by the ELISA. Analysis by ELISA of fractions obtained by gel filtration of plasma from a patient undergoing tPA treatment identified tPA/inhibitor complexes and free tPA but no low molecular weight degradation products of tPA. Determinations of tPA antigen were made at seven French clinical laboratories on coded and randomized plasma samples with known tPA antigen content. For undiluted samples there was no significant difference between the tPA levels found and those known to be present. The between-assay coefficient of variation was 7 to 10%. In conclusion, the ELISA appeared suited for determination of total tPA antigen in human plasma samples.

Lactulosuria in the Neonate: A Preliminary Report

PEDIATRICS ◽  
1965 ◽  
Vol 35 (2) ◽  
pp. 340-342
Author(s):  
JOYCE D. GRYBOSKI ◽  
JOHN J. BOEHM
Keyword(s):  
High Voltage ◽  
Preliminary Report ◽  
Milk Formula ◽  
Breast Fed ◽  
Electrophoresis Technique ◽  
High Voltage Electrophoresis

Urines from 121 neonates were examined for sugars by a high-voltage electrophoresis technique. Lactosuria and maltosuria occurred in 15.4% and 7.7% respectively of infants taking evaporated milk formula. Lactosuria occurred in 21.6% of infants receiving Similac and in 28.6% of the breast-fed infants. Lactulose, a sugar not previously described in urines of normal newborns, was present in the urine of 15.4% of infants taking evaporated milk and in 38.6% of infants taking Similac.

scholarly journals Parasitology and urban livestock farming in Nigeria : prevalence of ova in faecal and soil samples and animal ectoparasites in Makurdi

2007 ◽  
Vol 78 (1) ◽  
Author(s):  
E.A. Omudu ◽  
E.U. Amuta
Keyword(s):  
Test Tube ◽  
Soil Samples ◽  
Helminth Parasites ◽  
Livestock Farming ◽  
Chi Square ◽  
Zoonotic Infections ◽  
Faecal Samples ◽  
Significant Difference ◽  
Flotation Technique ◽  
Chi Square Analysis

Domestic environmental pollution resulting from urban livestock farming was investigated in Makurdi using parasitological techniques. The test tube flotation technique was used for the parasitological analysis of animal faecal matter and soil samples collected from residential premises. Ectoparasitic fauna of dogs, goats, sheep and cattle cohabiting with humans within the same residential compound were also collected and identified. The hand-picking and body brushing methods were employed to search for ticks, fleas, lice and mites. Of the 150 soil samples examined, 55 (36.7 %) were positive for 1 or more eggs of helminth parasites. There was no significant difference in the distribution of eggs in the soil samples from the 3 areas sampled (c2=0.046, df=2, P>0.05). Ascaris species were the dominant parasite eggs found. Of the 180 faecal samples examined, 107 (59.4 %) were positive for 1 or more eggs of helminth parasites. Chi-square analysis showed no significant difference in the level of infection of different animal faeces sampled (c2=5.74, df=4, P>0.05). Ascaris species were again the dominating helminth parasite eggs found. There was also no significant difference in the prevalence of helminth eggs in the animal faecal samples from the 3 areas sampled (c2=5.99, df=4, P>0.05). A total of 1908 ectoparasites was recovered (ticks: 32.80 %; lice: 22.43 %; fleas: 22.06% and mite: 22.69 %). There was no significant difference in infestation animals between sexes (c2=0.10, df=4, P>0.05). The predominant genus encountered for ticks were Amblyomma, while Linognathus (43.90%), Ctenocephalides (97.38%) and Sarcoptes (58.89 %) were most predominant for lice, fleas and mites respectively. The public health implications of the findings, especially as these relate to the increasing incidence and prevalence of zoonotic infections, are discussed.

scholarly journals Laser Speckle Imaging of Sensitized Acupoints

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Ning Ding ◽  
Jing Jiang ◽  
Xiaoxiao Liu ◽  
Yiyuan Xu ◽  
Jiatong Hu ◽  
...  
Keyword(s):  
Blood Perfusion ◽  
Laser Speckle ◽  
Control Group ◽  
Functional Changes ◽  
Pathological Conditions ◽  
Sd Rats ◽  
Significant Difference ◽  
Group A ◽  
Severity Of The Disease ◽  
Group B

Acupoints microcirculatory dynamics vary depending on the body’s health status. However, the functional changes observed during acupoint sensitization, that is, the disease-induced change from a “silenced” to an “activated” status, remain elusive. In this study, the microcirculatory changes at acupoints during sensitization were characterized. Thirty SD rats were randomly divided into five groups: normal control group (N), sham osteoarthritis group (S), light osteoarthritis group (A), mild osteoarthritis group (B), and heavy osteoarthritis group (C). The obtained results showed that the blood perfusion levels at the acupoints Yanglingquan (GB34), Zusanli (ST36), and Heding (EX-LE2) in groups A, B, and C were higher than those in groups N and S on days 14, 21, and 28 (p < 0.01 or p < 0.05). A significant difference in the blood perfusion was also observed at the acupoint Weizhong (BL40) in groups B and C on days 21 and 28 (p < 0.01). In addition, remarkable differences in the level of blood perfusion at the GB34, ST36, and EX-LE2 acupoints were observed on day 28 (p < 0.01 or p < 0.05) among groups A, B, and C. No marked differences in blood perfusion levels were observed at the nonacupoint site among all groups. In conclusion, acupoint sensitization is associated with an increase in the level of local blood perfusion at specific acupoints, and this increase is positively correlated with the severity of the disease. The functional changes in microcirculation at acupoints during sensitization reflect the different physiological and pathological conditions imposed by the disease.

scholarly journals Response of Soybean Pathogens to Glyceollin

2010 ◽  
Vol 100 (9) ◽  
pp. 897-903 ◽  
Author(s):  
Anatoly V. Lygin ◽  
Curtis B. Hill ◽  
Olga V. Zernova ◽  
Laura Crull ◽  
Jack M. Widholm ◽  
...  
Keyword(s):  
Hairy Root ◽  
Degradation Products ◽  
Macrophomina Phaseolina ◽  
Phytophthora Sojae ◽  
Plant Diseases ◽  
Phialophora Gregata ◽  
Diaporthe Phaseolorum ◽  
Significant Difference ◽  
Transformed Hairy Root ◽  
Soybean Genotypes

Plants recognize invading pathogens and respond biochemically to prevent invasion or inhibit colonization in plant cells. Enhancing this response in crop plants could improve sustainable methods to manage plant diseases. To enhance disease resistance in soybean, the soybean phytoalexin glyceollin was assessed in soybean hairy roots of two soybean genotypes, Spencer and PI 567374, transformed with either soybean isoflavone synthase (IFS2) or chalcone synthase (CHS6) genes that were inoculated with the soybean pathogens Diaporthe phaseolorum var. meridionales, Macrophomina phaseolina, Sclerotinia sclerotiorum, and Phytophthora sojae. The hairy-root-transformed lines had several-fold decreased levels of isoflavone daidzein, the precursor of glyceollin, and considerably lower concentrations of glyceollin induced by pathogens measured 5 days after fungal inoculation compared with the nontransformed controls without phenolic transgenes. M. phaseolina, P. sojae, and S. sclerotiorum grew much more on IFS2- and CHS6-transformed roots than on control roots, although there was no significant difference in growth of D. phaseolorum var. meridionales on the transformed hairy-root lines. In addition, glyceollin concentration was lower in D. phaseolorum var. meridionales-inoculated transformed and control roots than roots inoculated with the other pathogens. Glyceollin inhibited the growth of D. phaseolorum var. meridionales, M. phaseolina, P. sojae, S. sclerotiorum, and three additional soybean pathogens: Cercospora sojina, Phialophora gregata, and Rhizoctonia solani. The most common product of glyceollin conversion or degradation by the pathogens, with the exception of P. sojae, which had no glyceollin degradation products found in the culture medium, was 7-hydroxyglyceollin.

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