scholarly journals Females with impaired ovarian function could be vulnerable to environmental pollutants: identification via next-generation sequencing of the vaginal microbiome

Author(s):  
Seongmin Kim ◽  
Se Hee Lee ◽  
Kyung Jin Min ◽  
Sanghoon Lee ◽  
Jin Hwa Hong ◽  
...  
2019 ◽  
Author(s):  
Seongmin Kim ◽  
Se Hee Lee ◽  
Kyung Jin Min ◽  
Sanghoon Lee ◽  
Jin Hwa Hong ◽  
...  

Abstract Background: The vaginal microbiome has been widely investigated because of its utility in the diagnosis of vaginitis and its connection to various conditions. However, its relationship with impaired ovarian function and the influence of environmental pollutants have not been evaluated. Here, we conducted a next-generation sequencing study of the vaginal microbiome in young females with normal and decreased ovarian function and analyzed its correlation with various environmental pollutants. Methods: Vaginal swabs were collected from 92 individuals (22 with impaired ovarian function and 70 with normal function), and the vaginal microbiome was analyzed using next-generation sequencing. The 16S rDNA sequences were assembled by Fast Length Adjustment of SHort reads (FLASH) and clustered by Operational Taxonomic Units (OTUs). Diversity analysis was performed using Quantitative Insights Into Microbial Ecology (QIIME). Laboratory findings, including hormonal status were measured. Results: Most characteristics of the two groups were similar, except that the impaired function group showed lower AMH and higher FSH (p < 0.01 and p = 0.04, respectively). Among the most abundant taxa, several species differed; however, only two species showed significant differences: Propionibacterium acnes and Prevotella copri. The heat map did not show significant differences between the groups. Canonical correlation analysis revealed that more environmental pollutants were related to changes in the vaginal microbiome in the impaired ovarian function group than in the normal group. Conclusion: The vaginal microbiomes in young women with decreased ovarian function tended to be more sensitive to various environmental pollutants, especially volatile organic compounds (VOCs). Therefore, females with impaired ovarian function could be more vulnerable to VOCs.


2012 ◽  
Vol 9 (5) ◽  
pp. 965-976 ◽  
Author(s):  
Jennifer M. Fettweis ◽  
Myrna G. Serrano ◽  
Philippe H. Girerd ◽  
Kimberly K. Jefferson ◽  
Gregory A. Buck

ChemInform ◽  
2012 ◽  
Vol 43 (31) ◽  
pp. no-no
Author(s):  
Jennifer M. Fettweis ◽  
Myrna G. Serrano ◽  
Philippe H. Girerd ◽  
Kimberly K. Jefferson ◽  
Gregory A. Buck

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9684
Author(s):  
Nkechi Martina Odogwu ◽  
Oladapo O. Olayemi ◽  
Akinyinka O. Omigbodun

Accurate characterization of the vaginal microbiome remains a fundamental goal of the Human Microbiome project (HMP). For over a decade, this goal has been made possible deploying high-throughput next generation sequencing technologies (NGS), which indeed has revolutionized medical research and enabled large-scale genomic studies. The 16S rRNA marker-gene survey is the most commonly explored approach for vaginal microbial community studies. With this approach, prior studies have elucidated substantial variations in the vaginal microbiome of women from different ethnicities. This review provides a comprehensive account of studies that have deployed this approach to describe the vaginal microbiota of African women in health and disease. On the basis of published data, the few studies reported from the African population are mainly in non-pregnant post pubertal women and calls for more detailed studies in pregnant and postnatal cohorts. We provide insight on the use of more sophisticated cutting-edge technologies in characterizing the vaginal microbiome. These technologies offer high-resolution detection of vaginal microbiome variations and community functional capabilities, which can shed light into several discrepancies observed in the vaginal microbiota of African women in an African population versus women of African descent in the diaspora.


2020 ◽  
Vol 11 (05) ◽  
pp. 232-238
Author(s):  
Marcus Kleber

ZUSAMMENFASSUNGDas kolorektale Karzinom (KRK) ist einer der häufigsten malignen Tumoren in Deutschland. Einer frühzeitigen Diagnostik kommt große Bedeutung zu. Goldstandard ist hier die Koloskopie. Die aktuelle S3-Leitlinie Kolorektales Karzinom empfiehlt zum KRK-Screening den fäkalen okkulten Bluttest. Für das Monitoring von Patienten vor und nach Tumorresektion werden die Messung des Carcinoembryonalen Antigens (CEA) und der Mikrosatellitenstabilität empfohlen. Für die Auswahl der korrekten Chemotherapie scheint derzeit eine Überprüfung des Mutationsstatus, mindestens des KRAS-Gens und des BRAF-Gens, sinnvoll zu sein. Eine Reihe an neuartigen Tumormarkern befindet sich momentan in der Entwicklung, hat jedoch noch nicht die Reife für eine mögliche Anwendung in der Routinediagnostik erreicht. Den schnellsten Weg in die breite Anwendung können Next-Generation-Sequencing-basierte genetische Tests finden.


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