Assessing the individual and combined use of Caesalpinia coriaria (Plantae: Fabaceae) and Duddingtonia flagrans (Fungi: Orbiliaceae) as sustainable alternatives of control of sheep parasitic nematodes

2022 ◽  
pp. 1-15
Author(s):  
Luzlady Chavarría-Joya ◽  
Miguel Ángel Alonso-Díaz ◽  
Agustín Olmedo-Juárez ◽  
Elke von Son-de Fernex ◽  
Pedro Mendoza-de-Gives
Keyword(s):  
Parasitic Nematodes ◽  
Duddingtonia Flagrans ◽  
Combined Use ◽  
The Individual

Probing of the combined effect of bisquaternary ammonium antimicrobial agents and acetylsalicylic acid on model phospholipid membranes: differential scanning calorimetry and mass spectrometry studies

2014 ◽  
Vol 10 (12) ◽  
pp. 3155-3162 ◽  
Author(s):  
N. A. Kasian ◽  
V. A. Pashynska ◽  
O. V. Vashchenko ◽  
A. O. Krasnikova ◽  
A. Gömöry ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Differential Scanning Calorimetry ◽  
Acetylsalicylic Acid ◽  
Antimicrobial Agents ◽  
Combined Effect ◽  
Phospholipid Membranes ◽  
Scanning Calorimetry ◽  
Combined Use ◽  
The Individual

Qualitatively different membranotropic effects of the individual drugs and complexes formed in their combined use were demonstrated by differential scanning calorimetry.

scholarly journals Feasibility of mini combined cycles for naval applications

2018 ◽  
Vol 245 ◽  
pp. 07008 ◽  
Author(s):  
Dario Barsi ◽  
Carlo Costa ◽  
Francesca Satta ◽  
Pietro Zunino ◽  
Vitaly Sergeev
Keyword(s):  
Energy Production ◽  
High Efficiency ◽  
Combined Cycle ◽  
Pollutant Emissions ◽  
Large Power ◽  
Combined Use ◽  
Combined Cycles ◽  
Definition Of ◽  
The Individual ◽  
Near Future

The objective of energy production with low environmental impact will have, in the near future, high potential of development also for naval applications. The containment of pollutant emissions can be achieved by the combined use of an innovative mini gas-steam combined cycle with thermal energy cogeneration to feed the ship thermal utilities, in place of the current Diesel engine application, and liquefied natural gas as fuel (LNG). The present work is focused on the definition of the architecture of the plant, by selecting optimal distribution of pressure and temperature and repartition of power between Gas Turbine (GT), Steam Turbine (ST) and thermal utilities, as well as on the choice and sizing of the individual components. The main purpose is the definition of a compact, high efficiency, system. The proposed basic mini-cycle ranges from 2 MW to 10 MW electric power. Thanks to the combined heat and power cogeneration plant adopted, for an overall electrical efficiency of about 30%, a total return (thermal + electricity) of about 75% can be achieved. An example of plant providing large power, in a partially modular arrangement is also proposed.

scholarly journals Mixed Production of Filamentous Fungal Spores for Preventing Soil-Transmitted Helminth Zoonoses: A Preliminary Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
M. S. Arias ◽  
C. F. Cazapal-Monteiro ◽  
J. Suárez ◽  
S. Miguélez ◽  
I. Francisco ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Fungal Spores ◽  
Fungal Growth ◽  
Mucor Circinelloides ◽  
Parasitic Infections ◽  
Duddingtonia Flagrans ◽  
Liquid Media ◽  
Predator Species ◽  
Third Stage ◽  
The Individual

Helminth zoonoses are parasitic infections shared by humans and animals, being the soil-transmitted helminths (STHs) mainly caused by roundworms (ascarids) and hookworms. This study was aimed to assess the individual and/or mixed production of two helminth-antagonistic fungi, one ovicide (Mucor circinelloides) and other predator (Duddingtonia flagrans). Fungi were grown both in Petri plates and in a submerged culture (composed by water, NaCl, Na2HPO4· 12 H2O, and wheat (Triticum aestivum)). A Fasciola hepatica recombinant protein (FhrAPS) was incorporated to the cultures to improve fungal production. All the cultured plates showed fungal growth, without difference in the development of the fungi when grown alone or mixed. High counts ofMucorspores were produced in liquid media cultures, and no significant differences were achieved regarding single or mixed cultures, or the incorporation of the FhrAPS. A significantly higher production ofDuddingtoniaspores after the incorporation of the FhrAPS was observed. When analyzing the parasiticide efficacy of the fungal mixture, viability ofT. caniseggs reduced to 51%, and the numbers of third stage cyathostomin larvae reduced to 4%. It is concluded, the capability of a fungal mixture containing an ovicide (Mucor) and a predator species (Duddingtonia) for growing together in a submerged medium containing the FhrAPS offers a very interesting tool for preventing STHs.

Induction of traps by Ostertagia ostertagi larvae, chlamydospore production and growth rate in the nematode-trapping fungus Duddingtonia flagrans

1996 ◽  
Vol 70 (4) ◽  
pp. 291-297 ◽  
Author(s):  
J. Grønvold ◽  
P. Nansen ◽  
S.A. Henriksen ◽  
M. Larsen ◽  
J. Wolstrup ◽  
...  
Keyword(s):  
Biological Control ◽  
Growth Rate ◽  
Domestic Animals ◽  
Ageing Process ◽  
Parasitic Nematodes ◽  
Duddingtonia Flagrans ◽  
Ostertagia Ostertagi ◽  
Trap Formation ◽  
Nematode Larvae

AbstractBiological control of parasitic nematodes of domestic animals can be achieved by feeding host animals chlamydospores of the nematode-trapping fungus Duddingtonia flagrans. In the host faeces, D. flagrans develop traps that may catch nematode larvae. In experiments on agar, D. flagrans had a growth rate between 15 and 60 mm/week at temperatures between 20 and 30°C. The presence of nematodes induces the fungus to produce traps. The rate of trap formation in D. flagrans has an optimum at 30°C, producing 700–800 traps/cm2/2 days, when induced by 20 nematodes/cm2 on agar. Approaching 10 and 35°C the ability to produce traps is gradually reduced. The response of chlamydospore production on agar to changes in temperature is the same as that for trap formation. On agar, at 10, 20 and 30°C D. flagrans loses its trap inducibility after 2–3 weeks. During the ageing process, increasing numbers of chlamydospores are produced up to a certain limit. The time for reaching maximum chlamydospore concentration coincided with the time for loss of induction potential. The implications of these results in relation to biological control in faeces are discussed.

scholarly journals Performance of pre-sprouted sugarcane seedlings in response to the application of humic acid and plant growth-promoting bacteria

2018 ◽  
Vol 39 (3) ◽  
pp. 1365
Author(s):  
Hend Pereira de Oliveira ◽  
Raphael Oliveira de Melo ◽  
Marihus Altoé Baldotto ◽  
Messias Antônio Andrade ◽  
Lílian Estrela Borges Baldotto
Keyword(s):  
Humic Acid ◽  
Plant Growth ◽  
New Technology ◽  
Plant Growth Promoting Bacteria ◽  
Multiplication Rate ◽  
Combined Use ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
The Individual ◽  
Seedling Characteristics

Sugarcane cultivation using pre-sprouted seedlings has decreased the number of stalks used per hectare, increased the multiplication rate, improved seedling health, and increased planting uniformity. However, because this system has been only recently proposed, the number of studies evaluating this new technology is still scarce, particularly those examining the effect of the application of growth-promoting substances on seedling characteristics during the production phase. Here, we hypothesized that it is possible to combine the plant growth-promoting activity of humic acid (HA) with the inoculation of selected strains of plant growth-promoting bacteria (PGPB) to improve the yield of sugarcane seedlings compared with the individual application of these two techniques. The potential of the combined use of HA and PGPB as agricultural inputs was evaluated by conducting an experiment with sugarcane micro cuttings in a greenhouse. Treatments included control, PGPB, HA, and PGPB with HA. At the end of the experiment (60 d after planting), the plants were subjected to biometric evaluation. The results indicated that it was possible to combine HA with selected strains of PGPB (Burkholderia sp.) to improve the yield of seedlings compared with individual HA and PGPB treatments. HA, PGPB, and HA + PGPB increased the total dry matter compared with the control by 23%, 25%, and 36%, respectively. Therefore, the use of HA-based plant regulators in combination with PGPB was more effective than the isolated use of these inputs for the treatment of micro cuttings and improvement of sugarcane seedling yield.

Antigenic variability in Trichuris trichiura populations

Parasitology ◽  
1998 ◽  
Vol 117 (4) ◽  
pp. 347-353 ◽  
Author(s):  
R. M. CURRIE ◽  
C. S. NEEDHAM ◽  
L. J. DRAKE ◽  
E. S. COOPER ◽  
D. A. P. BUNDY
Keyword(s):  
West Indies ◽  
Plasma Sample ◽  
Trichuris Trichiura ◽  
Parasitic Nematodes ◽  
Male And Female ◽  
Antigenic Variability ◽  
Individual Worm ◽  
The Individual ◽  
Somatic Antigens

The present study examines antigenic variability for the human whipworm Trichuris trichiura. Recognition by IgG of somatic antigens of individual worms collected from 3 intensely infected children from Jamaica, West Indies has been investigated by immunoblotting. When probed with 1 plasma sample, significant differences in recognition of 2 selected antigens among worm populations and between male and female worms was observed. In addition, there was evidence for antigenic variability within worm populations at the individual worm level. Such variation may have considerable implications for the development of immunity to parasitic nematodes.

scholarly journals Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

2016 ◽  
Vol 65 (3-4) ◽  
pp. 13-20 ◽  
Author(s):  
Stanislav Simin ◽  
Simonida Đurić ◽  
Ljiljana Kuruca ◽  
Timea Hajnal-Jafari ◽  
Dragana Stamenov ◽  
...  
Keyword(s):  
Production Systems ◽  
Anthelmintic Resistance ◽  
Direct Interaction ◽  
Poor Performance ◽  
Gastrointestinal Nematodes ◽  
Fungal Species ◽  
Parasitic Nematodes ◽  
Current Experiment ◽  
Duddingtonia Flagrans ◽  
Reduction Percentage

Summary Gastro-intestinal nematodes (GIN) of sheep are one of major constraints in grazing production systems worldwide. Control is commonly achieved using anthelmintics, but global occurrence of anthelmintic resistance to different drugs and the emergence of multi-resistant GIN species seriously limit the efficiency of their use. Therefore, integrated parasite management is widely recommended, with nematophagous fungi as one of control tools. Duddingtonia flagrans is one of the most used species, with various effect of different isolates. In previously performed coproculture assay, we showed low efficacy of D. flagrans MUCL 9827 against infective larvae (L3) of sheep GIN. The aim of current experiment was to reevaluate its nematophagous potential, using the medium where direct interaction between the fungus and L3 could be observed. Nematophagous activity was tested on 2% water agar with addition of chloramphenicol on three series of plates seeded with 500 and 1000 chlamidospores and agar blocks with 7 days old mycelium. At Days 0 and 5, 500 L3 of sheep GIN were added to test the trapping activity. The cultures, including control plates with only L3, were incubated at 25°C for 10 days, followed by evaluation of their number and reduction percentage. Nematophagous activity of D. flagrans MUCL 9827 against L3 was clearly demonstrated. However, the overall efficacy was poor since trapping was observed only in one out of nine plates containing fungal material. Potential reasons for such poor performance of the isolate of fungal species, otherwise known as successful in trapping animal parasitic nematodes, are discussed.

In vitro biological control of bovine parasitic nematodes by Arthrobotrys cladodes, Duddingtonia flagrans and Pochonia chlamydosporia under different temperature conditions

2020 ◽  
Vol 94 ◽  
Author(s):  
Ítalo Stoupa Vieira ◽  
Isabela de Castro Oliveira ◽  
Artur Kanadani Campos ◽  
Jackson Victor de Araújo
Keyword(s):  
Biological Control ◽  
Mycelial Growth ◽  
Nematophagous Fungi ◽  
Nematicidal Activity ◽  
Parasitic Nematodes ◽  
Duddingtonia Flagrans ◽  
Pochonia Chlamydosporia ◽  
Fungal Strains ◽  
Temperature Conditions

Abstract Variations in temperature can affect the development of nematophagous fungi, especially when they are used in the biological control of parasitic nematodes in the pastures where cattle are reared. The aim of this work was to evaluate the effects of temperature on the performance of nematophagous fungi in the biological control of bovine parasitic nematodes. The mycelial growth, chlamydospore production and nematicidal activity of Duddingtonia flagrans, Arthrobotrys cladodes and Pochonia chlamydosporia were evaluated at 15, 20, 25, 30 and 35°C. The fungal strains achieved mycelial growth, chlamydospore production and nematicidal activity on parasitic nematodes under all temperature conditions tested. The fungi showed higher growth at intermediate temperatures (20, 25 and 30°C) than at the extremes of 15 and 35°C. At 25 and 30°C, D. flagrans realized 96.8 and 94.5% nematicidal activity on bovine parasitic nematodes, respectively. Arthrobotrys cladodes effected nematicidal activity of 85.3 and 83.5%, at 20 and 25°C, respectively. At 20 and 30°C, P. chlamydosporia achieved nematicidal activity of 81.3 and 87.4%, respectively. The maximum chlamydospore production was reached at 20, 25 and 30°C for D. flagrans, at 20 and 25°C for A. cladodes and P. chlamydosporia. The results of this study demonstrated that the tested fungal strains of D. flagrans, A. cladodes and P. chlamydosporia, when used in the biological control of bovine parasitic nematodes, were not limited by in vitro temperature variations. Therefore, the use of these strains of fungi as biological control agents of parasitic nematodes is promising.

Sign in / Sign up

Export Citation Format

Share Document