The Effect of Ultrasound Pre-treatment on the Yield, Chemical Composition and Antioxidant Activity of Essential Oil from Wild Lavandula stoechas L.

2018 ◽  
Vol 21 (1) ◽  
pp. 253-263 ◽  
Author(s):  
Chekoual Lilia ◽  
Aissat Abdelkader ◽  
Ait-Kaci Aourahoun Karima ◽  
Benabdelkader Tarek
2018 ◽  
Vol 16 (S1) ◽  
pp. S109-S118 ◽  
Author(s):  
L. Chekoual ◽  
A. Aissat ◽  
K. Ait-Kaci Aourahoun ◽  
T. Benabdelkader

The paper presents research results concerning the influence of ultrasonic pre-treatment (time intervals of 10, 20, 30, 45, and 60 min) on the process of extraction of essential oils from wild Algerian Lavandula stoechas. Tests were carried out in two populations. A sonication pre-treatment time of 10 min followed by 90 min of hydrodistillation (US-HD) gives a yield of 1.60 ± 0.1% of Kodiat essential oils (KEO). This pre-treatment time proved to be the best when compared with other pretreatment times. However, overall best yield (1.03 ± 0.07%) of El-Kahla essential oils (EEO) was obtained at 60 min of pre-treatment. These yields are higher compared with those (0.64 ± 0.02% and 0.50 ± 0.02%) obtained after 180 min of only hydrodistillation (HD) for the two samples of Lavandula stoechas, respectively. About 90.69% and 89.36% of total compounds were identified using chromatography–mass spectrometry (GC– MS) in KEO and EEO treated by ultrasound versus untreated samples (89.26% and 76%, respectively). A qualitative and quantitative difference in chemical composition between the essential oils of treated and untreated samples of the two harvesting sites was found. The percentage of most of the major compounds (fenchone, camphor, 1,8-cineole, bornylacetate, myrtenyl-acetate, and viridiflorol) and other compounds identified is higher in treated samples. GC/FID (flame ionization detector) analysis of KEO showed that the percentage of fenchone and camphor as major components is greater at 60 and 20 min of ultrasonic (US) pretreatment, respectively, compared with HD and the other US pre-treatment times. Results of the present study clearly indicated that sonication treatments are effective in enhancing the antioxidant activity of Lavandula stoechas extracts and could be further explored for commercial purposes to benefit consumers and the environment.


Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.


Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2888
Author(s):  
Carmen M. S. Ambrosio ◽  
Gloria L. Diaz-Arenas ◽  
Leidy P. A. Agudelo ◽  
Elena Stashenko ◽  
Carmen J. Contreras-Castillo ◽  
...  

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.


2019 ◽  
Vol 11 (4) ◽  
pp. 197
Author(s):  
Diogo Mendes da Silva ◽  
Suzan Kelly Vilela Bertolucci ◽  
Smail Aazza ◽  
Alexandre Alves de Carvalho ◽  
Simony Carvalho Mendonça ◽  
...  

The purpose of the present work was to evaluate the vegetative growth of Mentha piperita L. cultivated under different water availability, as well its influence in content, chemical composition and in vitro antioxidant activity of its essential oil. Plants were propagated by mother plants microcutting and scions were transplanted to 5 L pots with soil and cattle manure. Afterward, were kept at field capacity for 30 days and under treatment for 40 days. It was treated with different levels of water deficit treatments: (T1): 100 of field capacity (FC); (T2): 80 of FC; (T3): 60 of FC; (T4) 40 of FC with 5 blocks. Vegetative growth was evaluated by dry matter contents of all part of plants and by root/aerial rate. The essential oil of the leaves was extracted by hydrodistillation, analyzed by GC-FID and GC-MS and in vitro antioxidant potential was evaluated. A significant decrease in the dry matter of leaves and stems accompanied with a decrease in the roots dry matter was observed with an increase in the water stress. Quantitative chemical differences were observed in the chemical composition of the essential oil, according water availability. Total antioxidant activity showed a gradual increase as water stress progressed.


Author(s):  
Sudipta Jena ◽  
Asit Ray ◽  
Ambika Sahoo ◽  
Pradeep Kumar Kamila ◽  
Sanghamitra Nayak ◽  
...  

2020 ◽  
Vol 14 (7) ◽  
pp. 326-330
Author(s):  
P. R. E. Ribeiro ◽  
I. F. Montero ◽  
S. A. M. Saravia ◽  
V. P. Ferraz ◽  
R. A. Santos ◽  
...  

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