DIFFERENT TISSUE REACTIONS TO ACTIVATED CARBON FIVERS - PATHOLOGICAL AND IMMUNOLOGICAL FINDINGS AFTER SUBCUTANEOUS IMPLANTATION -

Author(s):  
Shozo Koyama ◽  
Satoshi Tanaka ◽  
Yoshihiro Yamaguchi ◽  
Hisao Haniu ◽  
Genichi Konishi ◽  
...  
2002 ◽  
Vol 388 (1) ◽  
pp. 167-171 ◽  
Author(s):  
Shozo Koyama ◽  
Satoshi Tanaka ◽  
Yoshihiro Yamaguchi ◽  
Hisao Haniu ◽  
Genichi Konishi ◽  
...  

Biomaterials ◽  
2005 ◽  
Vol 26 (18) ◽  
pp. 3901-3909 ◽  
Author(s):  
Gert W. Bos ◽  
Wim E. Hennink ◽  
Linda A. Brouwer ◽  
Wim den Otter ◽  
Theo F.J. Veldhuis ◽  
...  

2015 ◽  
Vol 14 (2) ◽  
pp. 70-75
Author(s):  
A. N. Ivanov ◽  
M. N. Kozadaev ◽  
D. M. Puchin'yan ◽  
Yu. E. Sal'kovskii ◽  
I. A. Norkin

The purpose of the study. The estimation of skin microcirculatory changes appearing in rats after subcutaneous implantation of polycaprolactone (PCL) scaffolds. Materials and methods. The experiments were conducted on 3 groups of rats: comparison group - the animals exposed to surgical intervention to the extent equivalent to matrix implantation, negative control group - animals subcutaneously implanted with non-biocompatible matrix; and experimental - rats subcutaneously implanted by PCL-scaffold. Skin microcirculation was analyzed by Lazer Doppler flowmetry (LDF) on the 7th, 14th and 21st day of the experiment. Morphological analysis of soft tissue complex and matrix samples was carried out on the 21st day of the experiment for scaffold biocompatibility verification. Results. It has been found that moderate increase in skin perfusion of animals in the comparison group over the surgical intervention area is not accompanied by the changes of active mechanisms of blood flow modulation and is completely resolved by the 21st day of the experiment. In negative control group, skin perfusion over the implantation area is 2 times higher than in controls in the period from 7th to 21st day of the experiment and this is accompanied by the significant increase of neurogenic and myogenic blood flow oscillation amplitudes. In the course of morphological analysis, these animals have demonstrated active inflammatory response. In the experimental group, perfusion changes are comparable with those in the comparison group and they resolve completely by the 21st day of the experiment. Morphological analysis suggests that by the 21st day of the experiment PLC-scaffold is evenly colonized by connective tissue cells and is vascularized. At the same time, reactive changes of adjacent tissues have not been detected. Conclusion. Skin microcirculatory changes over the scaffold implantation area correspond to the morphological pattern of tissue reactions which allows using LDF for dynamic estimation of scaffold biocompatibility in the course of subcutaneous implantation tests. This data suggest good PLC-scaffold biocompatibility which proves the prospects of its practical application in the tissue engineering.


2021 ◽  
Vol 22 (14) ◽  
pp. 7623
Author(s):  
Ignacio Stöwe ◽  
Jens Pissarek ◽  
Pia Moosmann ◽  
Annica Pröhl ◽  
Sven Pantermehl ◽  
...  

(1) Background: The aim of the present study was the biocompatibility analysis of a novel xenogeneic vascular graft material (PAP) based on native collagen won from porcine aorta using the subcutaneous implantation model up to 120 days post implantationem. As a control, an already commercially available collagen-based vessel graft (XenoSure®) based on bovine pericardium was used. Another focus was to analyze the (ultra-) structure and the purification effort. (2) Methods: Established methodologies such as the histological material analysis and the conduct of the subcutaneous implantation model in Wistar rats were applied. Moreover, established methods combining histological, immunohistochemical, and histomorphometrical procedures were applied to analyze the tissue reactions to the vessel graft materials, including the induction of pro- and anti-inflammatory macrophages to test the immune response. (3) Results: The results showed that the PAP implants induced a special cellular infiltration and host tissue integration based on its three different parts based on the different layers of the donor tissue. Thereby, these material parts induced a vascularization pattern that branches to all parts of the graft and altogether a balanced immune tissue reaction in contrast to the control material. (4) Conclusions: PAP implants seemed to be advantageous in many aspects: (i) cellular infiltration and host tissue integration, (ii) vascularization pattern that branches to all parts of the graft, and (iii) balanced immune tissue reaction that can result in less scar tissue and enhanced integrative healing patterns. Moreover, the unique trans-implant vascularization can provide unprecedented anti-infection properties that can avoid material-related bacterial infections.


2020 ◽  
Author(s):  
Feng Xiao ◽  
Bin Yao ◽  
Pavankumar Challa Sasi ◽  
Svetlana Golovko ◽  
Dana Soli ◽  
...  

2001 ◽  
Vol 11 (PR3) ◽  
pp. Pr3-279-Pr3-286
Author(s):  
X. Dabou ◽  
P. Samaras ◽  
G. P. Sakellaropoulos

2020 ◽  
Vol 64 (1-4) ◽  
pp. 1261-1268
Author(s):  
Shu Otani ◽  
Dang-Trang Nguyen ◽  
Kozo Taguchi

In this study, a portable and disposable paper-based microbial fuel cell (MFC) was fabricated. The MFC was powered by Rhodopseudomonas palustris bacteria (R. palustris). An activated carbon sheet-based anode pre-loaded organic matter (starch) and R. palustris was used. By using starch in the anode, R. palustris-loaded on the anode could be preserved for a long time in dry conditions. The MFC could generate electricity on-demand activated by adding water to the anode. The activated carbon sheet anode was treated by UV-ozone treatment to remove impurities and to improve its hydrophilicity before being loaded with R. palustris. The developed MFC could generate the maximum power density of 0.9 μW/cm2 and could be preserved for long-term usage with little performance degradation (10% after four weeks).


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