Cyclic Enzymatic Solid Phase Synthesis of Isotopically Labeled DNA Oligonucleotides

2009 ◽  
Vol 28 (11-12) ◽  
pp. 1030-1041 ◽  
Author(s):  
Ahmed M. Khan ◽  
Subrata H. Mishra ◽  
Markus W. Germann
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Dna Oligonucleotides

Thermolytic 4-Methylthio-1-butyl Group for Phosphate/Thiophosphate Protection in Solid-Phase Synthesis of DNA Oligonucleotides

2004 ◽  
Vol 69 (7) ◽  
pp. 2509-2515 ◽  
Author(s):  
Jacek Cieślak ◽  
Andrzej Grajkowski ◽  
Victor Livengood ◽  
Serge L. Beaucage
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Dna Oligonucleotides ◽  
Butyl Group

Synthesis and Screening of A DNA-Encoded Library of Non-Peptidic Macrocycles

2020 ◽  
Author(s):  
Eric Koesema ◽  
Animesh Roy ◽  
Nicholas G. Paciaroni ◽  
Thomas Kodadek
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Cell Permeability ◽  
Phase Synthesis ◽  
Protein Ligands ◽  
High Affinity ◽  
Scaffold Diversity

There is considerable interest in the development of libraries of non-peptidic macrocycles as a source of ligands for difficult targets. We report here the solid-phase synthesis of a DNA-encoded library of several hundred thousand thioether-linked macrocycles. The library was designed to be highly diverse with respect to backbone scaffold diversity and to minimize the number of amide N-H bonds, which compromise cell permeability. The utility of the library as a source of protein ligands is demonstrated through the isolation of compounds that bind streptavidin, a model target, with high affinity.

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