Ethanol production from alkali-pretreated oil palm empty fruit bunch by simultaneous saccharification and fermentation withmucor indicus

2016 ◽  
Vol 13 (6) ◽  
pp. 566-572 ◽  
Author(s):  
Abdi Christia ◽  
Arima Diah Setiowati ◽  
Ria Millati ◽  
Keikhosro Karimi ◽  
Muhammad Nur Cahyanto ◽  
...  
Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1944 ◽  
Author(s):  
Nur Md Razali ◽  
Mohamad Ibrahim ◽  
Ezyana Kamal Bahrin ◽  
Suraini Abd-Aziz

This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of biobutanol concentration and 0.10 g/g of biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of biobutanol concentration equivalent to 3.97 g/L and biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of biobutanol production, especially in consolidated bioprocessing technology.


2016 ◽  
Vol 6 (1) ◽  
pp. 773-779
Author(s):  
Leonard Guimarães Carvalho ◽  
Luiz Felipe A. Modesto ◽  
Donato A. Gomes Aranda ◽  
Nei Pereira Jr

Brazil presents the world's largest potential for the production of palm oil due to nearly 75 million hectares of land suitable for palm culture and advantageous soil and climate. The biomass generated in the production of palm oil (palm pressed fiber, PPF) is mainly composed of lignocellulosic material that can be hydrolyzed into fermentable sugars for further conversion to ethanol. This work evaluated alkaline pretreatment of this palm oil residue and subsequent Simultaneous Saccharification and Fermentation (SSF), achieving a conversion of glucose to ethanol higher than 90% and a concentration equivalent to 22.40 g/L of the alcohol.  


2020 ◽  
Vol 10 (17) ◽  
pp. 5987 ◽  
Author(s):  
Andre Fahriz Perdana Harahap ◽  
Jabosar Ronggur Hamonangan Panjaitan ◽  
Catia Angli Curie ◽  
Muhammad Yusuf Arya Ramadhan ◽  
Penjit Srinophakun ◽  
...  

Oil palm empty fruit bunch (OPEFB) is a potential raw material abundantly available for bioethanol production. However, the second-generation bioethanol is still not yet economically feasible. The COVID-19 pandemic increases the demand for ethanol as the primary ingredient of hand sanitisers. This study evaluates the techno-economic feasibility of hand sanitiser production using OPEFB-based bioethanol. OPEFB was alkaline-pretreated, and simultaneous saccharification and fermentation (SSF) was then performed by adding Saccharomyces cerevisiae and cellulose enzyme. The cellulose content of the OPEFB increased from 39.30% to 63.97% after pretreatment. The kinetic parameters of the OPEFB SSF at 35 °C, which included a µ max, ks, and kd of 0.018 h−1, 0.025 g/dm3, and 0.213 h−1, respectively, were used as input in SuperPro Designer® v9.0. The total capital investment (TCI) and annual operating costs (AOC) of the plant were $645,000 and $305,000, respectively, at the capacity of 2000 kg OPEFB per batch. The batch time of the modelled plant was 219 h, with a total annual production of 32,506.16 kg hand sanitiser. The minimum hand sanitiser selling price was found to be $10/L, achieving a positive net present value (NPV) of $108,000, showing that the plant is economically feasible.


2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH,50°C,48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.


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