Three-dimensional (3D) velocity map imaging: from technique to application

Author(s):  
Gihan Basnayake ◽  
Yasashri Ranathunga ◽  
Suk Kyoung Lee ◽  
Wen Li

Abstract The velocity map imaging (VMI) technique was first introduced by Eppink and Parker in 1997, as an improvement to the original ion imaging method by Houston and Chandler in 1987. The method has gained huge popularity over the past two decades and has become a standard tool for measuring high-resolution translational energy and angular distributions of ions and electrons. VMI has evolved gradually from 2D momentum measurements to 3D measurements with various implementations and configurations. The most recent advancement has brought unprecedented 3D performance to the technique in terms of resolutions (both spatial and temporal), multi-hit capability as well as acquisition speed while maintaining many attractive attributes afforded by conventional VMI such as being simple, cost-effective, visually appealing and versatile. In this tutorial we will discuss many technical aspects of the recent advancement and its application in probing correlated chemical dynamics.

2009 ◽  
Vol 80 (8) ◽  
pp. 083301 ◽  
Author(s):  
S. Kauczok ◽  
N. Gödecke ◽  
A. I. Chichinin ◽  
M. Veckenstedt ◽  
C. Maul ◽  
...  

2019 ◽  
Author(s):  
Xu Zhang ◽  
Jing Niu ◽  
Guipeng Li ◽  
Qionghai Dai ◽  
Dayong Jin ◽  
...  

ABSTRACTThere is increasing interest in understanding how the three-dimensional organization of the genome is regulated. Different strategies have been employed to identify chromatin interactions genome wide. However, due to the current limitations in resolving genomic contacts, visualization and validation of these genomic loci with sub-kilobase resolution remain the bottleneck for many years. Here, we describe Tn5 transposase-based Fluorescence in situ Hybridization (Tn5-FISH), a Polymerase Chain Reaction (PCR)-based, cost-effective imaging method, which achieved the co-localization of genomic loci with sub-kilobase resolution, to fine dissect genome architecture at sub-kilobase resolution and to verify chromatin interactions detected by Chromatin Configuration Capture (3C)-derivative methods. Especially, Tn5-FISH is very useful to verify short-range chromatin interactions inside of contact domain and Topologically Associated Domain (TAD). It also offers one powerful molecular diagnosis tool for clinical detection of cytogenetic changes in cancers.


Author(s):  
Nora Rat ◽  
Iolanda Muntean ◽  
Diana Opincariu ◽  
Liliana Gozar ◽  
Rodica Togănel ◽  
...  

Development of interventional methods has revolutionized the treatment of structural cardiac diseases. Given the complexity of structural interventions and the anatomical variability of various structural defects, novel imaging techniques have been implemented in the current clinical practice for guiding the interventional procedure and for selection of the device to be used. Three– dimensional echocardiography is the most used imaging method that has improved the threedimensional assessment of cardiac structures, and it has considerably reduced the cost of complications derived from malalignment of interventional devices. Assessment of cardiac structures with the use of angiography holds the advantage of providing images in real time, but it does not allow an anatomical description. Transesophageal Echocardiography (TEE) and intracardiac ultrasonography play major roles in guiding Atrial Septal Defect (ASD) or Patent Foramen Ovale (PFO) closure and device follow-up, while TEE is the procedure of choice to assess the flow in the Left Atrial Appendage (LAA) and the embolic risk associated with a decreased flow. On the other hand, contrast CT and MRI have high specificity for providing a detailed description of structure, but cannot assess the flow through the shunt or the valvular mobility. This review aims to present the role of modern imaging techniques in pre-procedural assessment and intraprocedural guiding of structural percutaneous interventions performed to close an ASD, a PFO, an LAA or a patent ductus arteriosus.


2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.


Author(s):  
Alexei Chichinin ◽  
Christof Maul ◽  
Karl-Heinz Gericke

The photodissociation dynamics of PCl3 at 235 nm has been studied by monitoring ground state Cl(2P3/2) and spin-orbitally excited Cl(2P1/2) atoms by resonance enhanced multiphoton ionization(REMPI). Also, the PCl+n (n=0,1,2)...


2020 ◽  
Author(s):  
A.I. Chichinin ◽  
C. Maul ◽  
K.-H. Gericke

2021 ◽  
Vol 13 (12) ◽  
pp. 2351
Author(s):  
Alessandro Torresani ◽  
Fabio Menna ◽  
Roberto Battisti ◽  
Fabio Remondino

Mobile and handheld mapping systems are becoming widely used nowadays as fast and cost-effective data acquisition systems for 3D reconstruction purposes. While most of the research and commercial systems are based on active sensors, solutions employing only cameras and photogrammetry are attracting more and more interest due to their significantly minor costs, size and power consumption. In this work we propose an ARM-based, low-cost and lightweight stereo vision mobile mapping system based on a Visual Simultaneous Localization And Mapping (V-SLAM) algorithm. The prototype system, named GuPho (Guided Photogrammetric System) also integrates an in-house guidance system which enables optimized image acquisitions, robust management of the cameras and feedback on positioning and acquisition speed. The presented results show the effectiveness of the developed prototype in mapping large scenarios, enabling motion blur prevention, robust camera exposure control and achieving accurate 3D results.


Energies ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2338
Author(s):  
Sofia Agostinelli ◽  
Fabrizio Cumo ◽  
Giambattista Guidi ◽  
Claudio Tomazzoli

The research explores the potential of digital-twin-based methods and approaches aimed at achieving an intelligent optimization and automation system for energy management of a residential district through the use of three-dimensional data model integrated with Internet of Things, artificial intelligence and machine learning. The case study is focused on Rinascimento III in Rome, an area consisting of 16 eight-floor buildings with 216 apartment units powered by 70% of self-renewable energy. The combined use of integrated dynamic analysis algorithms has allowed the evaluation of different scenarios of energy efficiency intervention aimed at achieving a virtuous energy management of the complex, keeping the actual internal comfort and climate conditions. Meanwhile, the objective is also to plan and deploy a cost-effective IT (information technology) infrastructure able to provide reliable data using edge-computing paradigm. Therefore, the developed methodology led to the evaluation of the effectiveness and efficiency of integrative systems for renewable energy production from solar energy necessary to raise the threshold of self-produced energy, meeting the nZEB (near zero energy buildings) requirements.


2013 ◽  
Vol 54 (9) ◽  
pp. 1096-1105 ◽  
Author(s):  
Ansgar Berg ◽  
Gottfried Greve

For the last three decades, two-dimensional (2D) echocardiography and Doppler echocardiography have been the primary imaging modalities for the diagnosis and management of heart disease in infants, children, and adolescents. These methods are non-invasive, highly sensitive, and cost-effective, and widely available, making them very useful in clinical work. During this period, the anatomic and hemodynamic abnormalities associated with different congenital and acquired pediatric heart diseases have been well outlined by echocardiography. Recent advances in computer technology, signal processing, and transducer design have allowed the capabilities of pediatric echocardiography to be expanded beyond qualitative 2D imaging and blood flow Doppler analysis. New modalities such as three-dimensional echocardiography, tissue Doppler imaging and speckle tracking echocardiography have been used to evaluate parameters such as ventricular volume, myocardial velocity, regional strain, and strain rate, providing new insight into cardiovascular morphology and ventricular systolic and diastolic function. Accordingly, a comprehensive and sophisticated quantification of ventricular function is now part of most echocardiography protocols. Use of measurements adjusted for body size and age is common practice today. These developments have further strengthened the position of echocardiography in pediatric cardiology.


Author(s):  
P A Bracewell ◽  
U R Klement

Piping design for ‘revamp’ projects in the process industry requires the retrieval of large amounts of ‘as-built’ data from existing process plant installations. Positional data with a high degree of accuracy are required. Photogrammetry, the science of measurement from photographs, was identified in Imperial Chemical Industries plc (ICI) as a suitable tool for information retrieval. The mathematical formulation enabling the definition of three-dimensional positions from photographic information is described. The process of using ICI's photogrammetric system for the definition of complete objects such as structures and pipes is illustrated. The need for specialized photogrammetric software for design purposes is explained. A case study describing how the photogrammetric system has been applied is described and graphical outputs from this exercise are shown. It is concluded that this particular photogrammetric system has proved to be a cost effective and accurate tool for the retrieval of ‘as-built’ information.


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