Effects of La2O3 contents on microstructure and properties of laser-cladded 5wt%CaB6/HA bioceramic coating

2022 ◽  
Author(s):  
Jinjing Liu ◽  
Xin Long ◽  
Hongmei Zhu ◽  
Weihua Zhu ◽  
Zhiyong Chen ◽  
...  
Keyword(s):  
Corrosion Resistance ◽  
Laser Cladding ◽  
Xrd Analysis ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Microstructure And Properties ◽  
Mg63 Cells ◽  
Bioceramic Coatings

Abstract In the present work, 5wt%CaB6/HA bioceramic coatings with different La2O3contents (0-0.6wt%) have been fabricated by a laser cladding technique on Ti-6Al-4V. The effects of La2O3 contents on microstructure and properties of the laser-cladded 5wt%CaB6/HA coatings have been carefully investigated. The results show that the microstructure is obviously refined, and the structure is relatively uniform after doping 0.2-0.4 wt% La2O3. As the La2O3 content increases, the corrosion resistance are found to increase firstly and then gradually decrease. The XRD analysis confirms that the amount of HA and TCP in the coating reaches maximum after doping 0.2wt% La2O3. The La2O3-doped coatings show a significantly higher bone-like apatite precipitation after immersion in SBF compared with La2O3-free coating. In vitro experiment also shows that 5wt%CaB6/HA bioceramic coatings with 0.2-0.4wt% La2O3 are more suitable for the attachment and proliferation of MG63 cells, exhibiting superior bioactivity and biocompatibility.

In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2

2019 ◽  
Vol 16 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Nasrin Zarkar ◽  
Mohammad Ali Nasiri Khalili ◽  
Fathollah Ahmadpour ◽  
Sirus Khodadadi ◽  
Mehdi Zeinoddini
Keyword(s):  
In Silico ◽  
Catalytic Domain ◽  
Md Simulations ◽  
Special Importance ◽  
Native Form ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Pharmaceutical Protein ◽  
The Stability

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT) that genetically fused to the whole IL-2. Deamidation is the most important reaction for chemical instability of proteins occurs during manufacture and storage. Deamidation of asparagine residues occurs at a higher rate than glutamine residues. The structure of proteins, temperature and pH are the most important factors that influence the rate of deamidation. Methods: Since there is not any information about deamidation of DAB389IL-2, we studied in silico deamidation by Molecular Dynamic (MD) simulations using GROMACS software. The 3D model of fusion protein DAB389IL-2 was used as a template for deamidation. Then, the stability of deamidated and native form of the drug was calculated. Results: The results of MD simulations were showed that the deamidated form of DAB389IL-2 is more unstable than the normal form. Also, deamidation was carried by incubating DAB389IL-2, 0.3 mg/ml in ammonium hydrogen carbonate for 24 h at 37o C in order to in vitro experiment. Conclusion: The results of in vitro experiment were confirmed outcomes of in silico study. In silico and in vitro experiments were demonstrated that DAB389IL-2 is unstable in deamidated form.

Effect of bleaching on ethyl glucuronide in hair: An in vitro experiment

2010 ◽  
Vol 198 (1-3) ◽  
pp. 23-27 ◽  
Author(s):  
Luca Morini ◽  
Alessandra Zucchella ◽  
Aldo Polettini ◽  
Lucia Politi ◽  
Angelo Groppi
Keyword(s):  
Ethyl Glucuronide ◽  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals A review of biocompatibility of zirconia: In vitro experiment

2018 ◽  
Vol 56 (4) ◽  
pp. 391 ◽  
Author(s):  
Da-Won Suh ◽  
Young-Kyun Kim ◽  
Yang-Jin Yi
Keyword(s):  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals Stratified Community Responses to Methane and Sulfate Supplies in Mud Volcano Deposits: Insights from an In Vitro Experiment

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113004 ◽  
Author(s):  
Yu Zhang ◽  
Lois Maignien ◽  
Alina Stadnitskaia ◽  
Pascal Boeckx ◽  
Xiang Xiao ◽  
...  
Keyword(s):  
Mud Volcano ◽  
Community Responses ◽  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals Fast low-angle positive contrast steady-state free precession imaging of USPIO-labeled macrophages: theory and in vitro experiment

2009 ◽  
Vol 27 (7) ◽  
pp. 961-969 ◽  
Author(s):  
Nicole Mascheri ◽  
Rohan Dharmakumar ◽  
Zhuoli Zhang ◽  
Tatjana Paunesku ◽  
Gayle Woloschak ◽  
...  
Keyword(s):  
Steady State ◽  
Steady State Free Precession ◽  
Positive Contrast ◽  
Free Precession ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Free Precession Imaging

Comparison of Carotid Stents: An In-Vitro Experiment Focusing on Stent Design

2009 ◽  
Vol 16 (2) ◽  
pp. 168-177 ◽  
Author(s):  
Stefan Müller-Hülsbeck ◽  
Philipp J. Schäfer ◽  
Nikolas Charalambous ◽  
Silvio R. Schaffner ◽  
Martin Heller ◽  
...  
Keyword(s):  
Stent Design ◽  
Vitro Experiment ◽  
In Vitro Experiment

Wear and Corrosion Behaviours of FeCrNiSi Alloy Coatings by Laser Cladding

2017 ◽  
Vol 898 ◽  
pp. 1406-1413
Author(s):  
Yu Long Qi ◽  
Hai Yan Chen ◽  
Chen Yang Shu ◽  
Xuan Zhao ◽  
Li Hua Dong ◽  
...  
Keyword(s):  
Corrosion Resistance ◽  
Abrasive Wear ◽  
Laser Cladding ◽  
Wear Behavior ◽  
Testing Machine ◽  
Three Dimensional ◽  
Xrd Analysis ◽  
Hard Coating ◽  
Wear Testing ◽  
Surface Mapping

Soft and hard FeCrNiSi alloy coatings were obtained on 30CrMo alloy steel surface by laser cladding. The phase constitution, microstructure, frictional wear behavior and corrosion resistance of the composite coating were analyzed using X-ray diffraction (XRD), scanning electron microscope (SEM), three-dimensional non-contact surface mapping, friction and wear testing machine and electrochemical workstation, separately. XRD analysis showed that the cladding layer was mainly composed of Fe-based alloy composition, accompanied by a small amount of cobalt nickel alloy. There were massive protrusions in the interface of the soft sample, and the coating was regularly dendritic. Hard sample coating lines were cluttered, and there was no bulk deposition. Under the same wear condition, the soft coating exhibited serious abrasive wear, while the hard coating had slight abrasive wear behavior. The polarization curves in 3%NaCl solution revealed that the self-corrosion potential of the soft coating was positive shifted more than that the hard coating. The soft coating has better corrosion resistance than the hard coating.

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