scholarly journals The effect of VP3 Biofertilizer and its carrying materials on the germination of six plants

2021 ◽  
Vol 905 (1) ◽  
pp. 012004
Author(s):  
N Arfarita

Abstract This study investigated the growth after application of VP3 biofertilizer and its essential ingredients (vermiwash and vermicompost) and the effect of their pathogenicity on the germination of soybeans, green beans, long beans, kale, mustard greens, and green spinach. Research design was observed on plant height, root length, fresh weight, and signs of pathogenicity. The VP3 biofertilizer inoculation gave better results than other treatments on the high germination and fresh weight. However, in water spinach plants, the application of VP3 biofertilizer had a significant effect only on the height parameters of the seeds. Provision of VP3 biofertilizer and each of its carriers did not show any signs of pathogenicity such as necrosis, wilting, root rot, or stem rot. This finding indicates that VP3 biofertilizer is potential as a seed treatment and is also known to contain soil-borne disease controlling agent bacteria.

Plant Disease ◽  
1998 ◽  
Vol 82 (3) ◽  
pp. 294-299 ◽  
Author(s):  
W. Mao ◽  
R. D. Lumsden ◽  
J. A. Lewis ◽  
P. K. Hebbar

Bioassays were conducted in a greenhouse at 18°C to determine the effectiveness of a seed treatment used in combination with biocontrol agents for the reduction of corn damping-off caused by species of Pythium and Fusarium. Corn seeds were infiltrated with tap water, drained, air-dried, and then coated with biomass of an antagonistic fungus, Gliocladium virens isolate Gl-3, or an antagonistic bacterium, Burkholderia cepacia isolates Bc-B or Bc-1, or a combination of Gl-3 with each of the bacterial isolates. A nonsterile field soil was infested with a combination of pathogens: Pythium ultimum, P. arrhenomanes, and Fusarium graminearum at 2 inoculum rates (1× and 4×). Pre-infiltration enhanced (P ≤ 0.05) disease control with most treatments at both inoculum rates. Treatments with biocontrol agents alone or in combination, as well as the fungicide captan, effectively reduced the disease at a pathogen inoculum rate of 1×, resulting in greater (P ≤ 0.05) seedling stands, plant height, and fresh weight, and lower (P ≤ 0.05) root rot severity compared with untreated seeds in infested soil. At a pathogen inoculum rate of 4×, stands were lower (P ≤ 0.01) and root-rot severity was higher (P ≤ 0.01) compared to those at 1× for all treatments. Nevertheless, coating seeds with all biocontrol agents (alone or in combination), except with Bc-1 alone, reduced disease (P ≤ 0.05) compared to untreated seeds in infested soil. At both inoculum rates of 1× and 4×, coating seeds with Gl-3 + Bc-B was more effective (P ≤ 0.05) in disease control than any other treatment, resulting in stands, growth rate (plant height and fresh weight), and root rot severity similar to plants from untreated seeds in noninfested soil. In addition, when the exudate from a 2-h infiltration of corn seed was added to the seeds during seed coating, seedling stand was often lower and root rot severity was often higher than those from infiltrated seeds (P ≤ 0.05). These results indicated that the infiltration process removed certain exudates, including nutrients and/or stimulants (not detected in this study) that might be utilized by pathogens to initiate seed infection. A thin-layer chromatography (TLC) profile of the exudates showed the presence of eight amino acids and three major carbohydrates.


1971 ◽  
Vol 22 (2) ◽  
pp. 189 ◽  
Author(s):  
JW Meagher ◽  
SC Chambers

The effects of the cereal cyst nematode Heterodera arenae and the fungus Rhizoctonin solani on the growth of wheat seedlings were examined under glasshouse conditions. In combination, the pathogens caused significantly greater reduction in tillering, plant height, fresh weight, and root number and length than that caused by either pathogen when acting alone. Reduction in plant height, root number, and root length were the result of interaction. Nematode-fungus interaction may be the cause of disease in the field usually attributed to only one of these pathogens.


2018 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Daru Nurdianna ◽  
Retno Bandriyati Arni Putri ◽  
Dwi Harjoko

This study aims to determine the differences in the growth response of green curly lettuce to the differentiation treatment of LED lighting with different spectrum in indoor hydroponics. The research was conducted from October to November 2017. The experimental design used was experimental with 1 lighting factor with 5 levels, there are sunlight (P0), LED 20% blue: 80% red (P1), LED 80% blue: 20% red (P2), 50% blue LED: 50 % red (P3), and 100% white daylight (P4). Observational variables included plant height, leaf number, leaf area, leaf color, total wet weight, leaf wet weight, wet root weight and dry weight of the plant. The result of this experiment showed that LED irradiation have affect the treatment are plant height, leaf number, leaf area, chlorophyll content, fresh weight of harvest, root length, and dry weight of brackets. While those that have no significant effect are the wet weight of the roots. The results showed that between LED irradiation treatment L1, L2, L3, and L4 showed that treatment L2 with 80% blue spectrum composition: 20% red showed better growth based on: high plant, chlorophyll content, and root length. Treatment with L4 with white LED showed better growth based on the number of leaves, leaf area, fresh weight of harvest, root wet weight, and dry weight of total biomass. The results below the sunlight better than the LED irradiation treatment, because the intensity of LED far low and lack of controlled environmental conditions technology and management for growing lettuce. In all of LED treatment still looked etiolation because light intensity was not optimal yet.


2021 ◽  
Vol 22 (2) ◽  
Author(s):  
JANE KATHRYNE JOLANDA LAISINA ◽  
AWANG MAHARIJAYA ◽  
SOBIR SOBIR ◽  
AGUS PURWITO

Abstract. Laisina JKJ, Maharijaya A, Sobir, Purwito A. 2021. Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches. Biodiversitas 22: 537-545. Prediction of drought adaptive potatoes requires selection indicators. This study aimed to determine in vitro selection indicators for obtaining drought adaptive potato genotypes in vitro and in vivo. The in vitro study was performed using single-node explants from seven genotypes cultured for six weeks on MS medium added with 0.2 M sorbitol to achieve the research aims. The cultures were partially observed and followed by watering the in vitro tubing medium. For the in vivo study, drought stress was applied for 21 days when the plants reached 25 days after planting (DAP) and for 14 days when the plants reached 50 DAP, then plants were irrigated again until harvesting. The results showed that plant height and root length can be used as selection indicators because they had a relative decrease below 50%, a close relationship and had the same variance with the in vitro number of tubers, in vitro tuber fresh weight and in vivo tuber fresh weight in drought stress at 25 DAP. These characters were determinant characters for the clustering pattern in the heat map cluster analysis. Based on these characters, SSI analysis was also performed and it was found that the PKHT4 was more adaptive than the PKHT6 in vitro, but the two genotypes were classified in the medium adaptive in vivo. In conclusion, plant height and root length were determined as the in vitro selection indicators in predicting drought adaptive genotypes in vivo at the plant age of 25 DAP.


2018 ◽  
Author(s):  
DEWA OKA SUPARWATA

This research aimed to study the respond of the growth and production of green beans toward shade treatment. The research location is in Pentadio Timur, Telaga Biru Gorontalo, which was done for 3 months from Desember 2016 to Februari 2017. The research design is RAK or group randomized design, with four level of treatment, which is, P0 = without shade, P1 = 1 layer of paranet thickness treatment, P2 = 2 layer of paranet thickness treatment, P3 = 3 layer of paranet thickness treatment. These four treatments was repeated 3 times that obtains 12 trial plots. The data were analyzed through Anova with SAS data processing. The result of variant investigation analysis on BNT (P<0.05) showed that different treatment of shade is significant to the parameters of number of leaves on the 8th week, number of branch on the 6th and 8th week. Meanwhile parameters of plant height, number of pods, and number of seeds per pods are not significantly different.


2019 ◽  
Vol 4 (1) ◽  
pp. 8-15
Author(s):  
Rahman Hairuddin ◽  
S. Sachril

This study was aimed to increase the production of Thai eggplant (Solanum melongena L) grown at altitude of 2 masl using myccohirzal biofertilizer. The study was conducted in Experimental Farm of Agricultural Faculty of Campus II University of Cokroaminoto Palopo, Batu Pasi Sub-district, Wara Utara District, Palopo City. This research applied the method of Randomized Block Design with four replicants and five treatments; P0 = Without treatment (control), P1 = Mycorrhizal fertilizer of 5 gr/plant, P2 = Mycorrhizal fertilizer of 10 gr/plant, P3 = Mycorrhizal fertilizer of 15 gr/plant, and P4 = Mycorrhizal fertilizer of 20 gr/plant. Research of this study showed that one treatment significantly affected the root length of eggplant, namely P2 with root length of 39.42 cm. In general, application of mycorrhizal fertilizer did not significantly affect the growth and production of eggplant, but several doses resulted in better outcome. Treatment of P3 obtained the best plant height of 9.22 cm, while the best average flowering age was found in P2 and P4 of 31.8 days after planting. Moreover, P3 and P4 produced the most number of flower, namely 3.83 flowers, while the most number of fruits was obtained by P4 of 3.53 fruits. At last, P3 produced the heaviest fresh weight of fruit of 221.13 gram


Plant Disease ◽  
1998 ◽  
Vol 82 (12) ◽  
pp. 1402-1402 ◽  
Author(s):  
W. Msikita ◽  
B. James ◽  
H. T. Wilkinson ◽  
J. H. Juba

In diagnostic surveys conducted in parts of Benin and Nigeria to determine the incidence of pre-harvest cassava root and stem rot during the dry season, Macrophomina phaseolina (Tassi) Goidanich constituted 14.2 and 18.7% of the total fungi (n = 201) associated with cassava root and stem rot from Benin and Nigeria (1). Pathogenicity of M. phaseolina on cassava was tested with cv. Agric. Inocula for pathogenicity tests were prepared by incubating 5-mm-diameter mycelial plugs for each of five isolates (Mp 1 to Mp 5, all collected from Benin) with 500 ml of autoclaved, sterilized, dehusked rice seed for 14 days at 30°C. Five 30-cm-long stem portions per isolate were cut from healthy cassava, surface disinfested in hot water (52°C, 5 min), and planted into 1-liter pots containing autoclaved, sterilized sand mixed with 10 ml of air-dried inoculum. Five plants per isolate similarly treated but not inoculated served as controls. Plants were watered once a week, and maintained in a greenhouse under natural light at 28 to 30°C. Lower leaves of inoculated plants gradually wilted, usually preceded by chlorosis, and brown to black lesions formed on the lower stem portions of some roots. Control plants remained asymptomatic. Plant height and percentage of leaf wilt (determined by counting the number of leaves wilted per plant and dividing by the total number of leaves per plant) were measured on a weekly basis for 8 weeks for each of the control and inoculated plants. At the end of 8 weeks, lesion length on the lower stem was measured. There were significant differences (P < 0.05) in length of the lesions and percentage of leaf wilt induced by the different isolates of M. phaseolina. Isolate Mp 1 induced the longest lesion (7.2 cm), followed by Mp 4 (4.1 cm), Mp 3 and Mp 5 (3.8 cm each), and Mp 2 (1.2 cm). Mp 4 induced the highest percentage of wilted leaves (53%), followed by Mp 1, Mp 3, and Mp 5 (30%), and Mp 2 (10%). All five M. phaseolina isolates (except Mp 3) reduced plant height, compared with control treatments. M. phaseolina was isolated from all infected plants, and the identification was independently confirmed by the International Mycological Institute, Surrey, UK. This is the first report of M. phaseolina causing pre-harvest cassava root rot in Benin and Nigeria. Reference: (1) W. Msikita et. al. Plant Dis. 81:1332, 1997.


Author(s):  
Wiwik Yunidawati ◽  
Riyanti Riyanti ◽  
Mazlina Mazlina

This study aims to determine the effect of bio fertilizers and foliar fertilizers on growth and yield of celery (Apium graviolens). This research was conducted at the Experimental Garden of the Faculty of Agriculture, Amir Hamzah University, Medan, Medan Estate Village, Percut Sei Tuan District with a height of ± 25 meters above sea level, which was conducted from May to July 2020. This study used a factorial Randomized Block Design with two factors studied, namely the first factor was the concentration of bio fertilizer Impro Tanotec (H), which consisted of 4 levels, namely H0 (0 ml/l water), H1 (1,5 ml/l water), H2 (2,5 ml/l water), H3 (3,5 ml/l water), and the second factor is the concentration of foliar fertilizer Growmore (D) which consists of 4 levels, namely D0 (0 gr/lwater), D1 (1 gr/l water), D2 (2 gr/l water), and D3 (3 gr/l water). Parameters observed included plant height (cm), number of leaves, number of stalks, root length (cm), and fresh weight per plant. (gr). The results showed that the concentration of bio fertilizer had a very significant effect on plant height at the age of 20, 40, and 60 days after planting, the number of leaves at the age of 20, 40, and 60 days after planting, the number of stalks at the age of 40 and 60 days after planting, root length, and fresh weight per plant, but did not significantly affect the number of stalks at the age of 20 days after planting. The best bio-fertilizer concentration was obtained in the H3 treatment (3.5 ml/l water). The concentration of foliar fertilizer had a very significant effect on plant height at the age of 20, 40, and 60 days after planting, the number of leaves at the age of 20, 40, and 60 days after planting, the number of stalks at the age of 40 and 60 days after planting, and the fresh weight per plant, had a significant effect on root length, and had no significant effect on the number of stalks at the age of 20 days after planting. The best foliar fertilizer concentration was obtained in D3 treatment (3 gr/l water). The interaction between the concentration of bio fertilizer and foliar fertilizers had a significant effect on the number of leaves at the age of 40 and 60 days after planting, where the best treatment combination was obtained in the H3D3 treatment (bio fertilizer concentration 3.5 ml/l water and foliar fertilizer concentration 3 gr/l water).


Plant Disease ◽  
1997 ◽  
Vol 81 (5) ◽  
pp. 450-454 ◽  
Author(s):  
W. Mao ◽  
J. A. Lewis ◽  
P. K. Hebbar ◽  
R. D. Lumsden

Bioassays were conducted under greenhouse conditions to test the efficacy of antagonists applied to corn (Zea mays) seed for protection against seed rot and seedling damping-off at 18 and 25°C in a field soil artificially infested with a combination of Pythium ultimum, P. arrhenomanes, and Fusarium graminearum. Biomass of Gliocladium virens isolates Gl-3 or Gl-21, Trichoderma viride isolate Tv-1, or peat-based slurry of Burkholderia cepacia isolates Bc-B, Bc-T, or Bc-1 was coated individually onto corn seeds in one test, and Gl-3 or Bc-B at four inoculum levels was used in another test. Seed treatments with most of the biocontrol agents, as well as with the fungicide captan, significantly (P ≤ 0.05) increased seedling stand, plant height and fresh weight, and decreased root rot severity compared with untreated seeds in pathogen-infested soil. Coating seeds with the biocontrol fungus G. virens isolate Gl-3 was the most effective treatment, resulting in greater (P ≤ 0.05) seedling stand, plant height, and fresh weight, and lower (P ≤ 0.05) severity of root rot than those parameters from seeds treated with captan or other antagonists at both temperatures. The results from the seeds treated with Gl-3 were similar to those of untreated seeds in noninfested soil. In treatments with Bc-1, Bc-T, Bc-B, or Tv-1, incubation temperature affected plant emergence, root rot severity, plant height, and fresh weight (P ≤ 0.01). Conversely, in seeds coated with Gl-3 or Gl-21, these parameters were similar at both temperatures. The minimum number of propagules needed per corn seed to obtain plant emergence comparable to that from captan-treated seeds was between 104 and 105 CFU for Gl-3 and >108 for Bc-B. When propagules of Gl-3 were applied at a rate >106 CFU per seed, seedling emergence was greater (P ≤ 0.05) than that from captan-treated seeds.


1973 ◽  
Vol 51 (5) ◽  
pp. 1075-1077 ◽  
Author(s):  
J. T. A. Proctor

Five-minute exposure of radish plants to far-red radiation at the end of each day after the development of the first two leaves significantly increased plant height, leaf length and width, root length, fresh weight of leaves, and reduced the chlorophyll content of leaves.


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