scholarly journals Isolation Of Pseudomonas Aeruginosa from Soil and Production of Lipase Enzyme

2022 ◽  
Vol 961 (1) ◽  
pp. 012087
Author(s):  
A A Ali ◽  
K W Hameed ◽  
M I Nadder

Abstract The isolates of Pseudomonas aeruginosa bacteria were uncovered in the soil surrounding the roots of palms and public gardens in Baghdad for the production of lipase enzyme. The lipase enzyme has many applications that are included in the textile and food industry, and the manufacture of detergents and medical preparations. Several tests such as temperature change, incubation period, change of lipid sources, nitrogen sources such as peptone and tryptone, and carbon sources such as glucose and lactose were carried out to choose suitable conditions for bacterial growth. The results indicated studying the conditions affecting production, it was noted that the best production was when using the culture medium to which 1% of corn oil was added, pH 7, at a temperature of 37 °C and an incubation period of 24 hours in vibrating incubator at 151 rpm, The soil surrounding the roots of the plant is a good reservoir for the presence of Pseudomonas aeruginosa bacteria

2021 ◽  
Author(s):  
Janani Balraj ◽  
Thandeeswaran Murugesan ◽  
Vidhya Kalieswaran ◽  
Karunyadevi Jairaman ◽  
Devippriya Esakkimuthu ◽  
...  

Abstract Our earlier paper had established the fact that new soil fungi known as Cunninghamella blakesleeana is potent enough to produce lovastatin significantly. At present, there are no reports on the media optimization for the lovastatin production. Hence, the objective is to optimize the fermentation conditions for lovastatin production by Cunninghamella blakesleeana under Solid State fermentation (SSF) condition through screening the critical factors by one factor at a time and then, optimize the factors selected from screening using statistical approaches. SSF was carried using the pure culture of Cunninghamella blakesleeana KP780148.1 with wheat bran as substrate. Initial screening was performed for physical parameters, carbon sources and nitrogen sources and then optimized the selected parameters through PBD and BBD. Screening result indicated the optimum values of the analysed parameter for the maximal production of lovastatin by Cunninghamella blakesleeana were selected. Out of the nine factors MgSO4, (NH4)2SO4, pH and Incubation period were found to influence the lovastatin production significantly after PBD. The optimal levels of these variables and the effect of their mutual interactions on lovastatin production were determined using BBD surface design. The optimum medium composition was found to be MgSO4(0.2 g/L), (NH4)2 SO4 (12.5 g/L), pH (6) and Incubation period (7 days). Experimental studies showed a yield of 7.39 mg/g at the above optimized conditions which were observed to be very nearby to the predicted value and hence the model was successfully validated. Hence, this is the first report on the optimization of critical parameters for lovastatin production by Cunninghamella blakesleeana.


2014 ◽  
Vol 17 ◽  
pp. 180-193 ◽  
Author(s):  
M. Ziayoddin ◽  
Junna Lalitha ◽  
Manohar Shinde

The culture conditions for the production of extracellular agarase by Pseudomonas aeruginosa ZSL-2 were optimized using One-Factor-At-A-Time combined with orthogonal array design. One-Factor-At-A-Time method investigates the effect of time, temperature, NaCl, carbon sources, nitrogen sources and pH on agarase production. The optimized culture conditions obtained from the statistical analysis were temperature of 30 °C, pH 8.5, NH4NO3 2 g L-1 and agar 3 g L-1. The L9 orthogonal array design was used to select the fermentation parameters influencing the yield of agarase. The order of the factors affecting the fermentation process was found to be NH4NO3 > pH > agar > temperature, with temperature playing a significant role on the agarase production (p < 0.10). The higher yields than those in basal media culture were obtained in the final optimized medium with activity of 0.439 ± 0.013 U ml-1. Extracellular agarase hydrolysed agar into a range of oligosaccharides which were analysed by LC-ESI-MS spectrometry as anhydrogalactose, galactose, agarobiose, agarotetrose and agarohexaose.


Author(s):  
RIRIN PUSPADEWI ◽  
PUTRANTI ADIRESTUTI ◽  
MIRA A. DEWI ◽  
INNANI MUKARROMAH ◽  
YUNIAR RAHMADINNI

Objective: Lipase was protein compounds that can be used for many human activities. Its main function was to degrade fat including 'wrapping' cholesterol which make easily flowed in the blood. The presence lipase was important because can help the digestive healthy. These enzyme can catalyze a variety of reactions including hydrolysis, alcoholysis, esterification and aminolysis. Lipase was utilized in various sectors, such as fat, oil, milk and pharmaceutical industries. This enzyme biosynthesis can be carried out by Pseudomonas aeruginosa, Lactobacillus plantarum and Aspergillus niger. Methods: The process through fermentation techniques in lipid containing substrates under optimal conditions required by microorganisms. The fermentation products produced were tested for the presence of lipase enzymes qualitatively and quantitatively. The biosynthesis process can be influence by changes in pH, temperature and the presence of glucose. This study aimed to determine the ability of L. plantarum to produce lipases with vegetables oil substrates. The research used L. plantarum carried out at 37 °C for 24-48 h and pH 6-8 in the vegetable oil substrates. Results: The fermentation products showed hydrolysis reaction to the test media containing oil lipid with lipase levels of 2.708-3.3000 U/ml Conclusion: The results showed that Lactobacillus plantarum can synthesize the lipase enzyme in palm oil and corn oil as substrates.


2020 ◽  
Vol 21 (14) ◽  
pp. 1539-1550
Author(s):  
Nur S. Ismail ◽  
Suresh K. Subbiah ◽  
Niazlin M. Taib

Background: This is the fastest work in obtaining the metabolic profiles of Pseudomonas aeruginosa in order to combat the infection diseases which leads to high morbidity and mortality rates. Pseudomonas aeruginosa is a high versatility of gram-negative bacteria that can undergo aerobic and anaerobic respiration. Capabilities in deploying different carbon sources, energy metabolism and regulatory system, ensure the survival of this microorganism in the diverse environment condition. Determination of differences in carbon sources utilization among biofilm and non-biofilm of Pseudomonas aeruginosa provides a platform in understanding the metabolic activity of the microorganism. Methods: The study was carried out from September 2017 to February 2019. Four archive isolates forming strong and intermediate biofilm and non-biofilms producer were subcultured from archive isolates. ATCC 27853 P. aeruginosa was used as a negative control or non-biofilm producing microorganism. Biofilm formation was confirmed by Crystal Violet Assay (CVA) and Congo Red Agar (CRA). Metabolic profiles of the biofilm and non-biofilms isolates were determined by phenotype microarrays (Biolog Omnilog). Results and Discussion: In this study, Pseudomonas aeruginosa biofilm isolates utilized uridine, L-threonine and L-serine while non-biofilm utilized adenosine, inosine, monomethyl, sorbic acid and succinamic acid. Conclusion: The outcome of this result will be used for future studies to improve detection or inhibit the growth of P. aeruginosa biofilm and non-biofilm respectively.


2017 ◽  
Vol 6 (8) ◽  
pp. 5459
Author(s):  
Chandra Teja K. ◽  
Rahman S. J.

Entomopathogenic fungi like Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii are used in biological control of agricultural insect pests. Their specific mode of action makes them an effective alternative to the chemical Insecticides. Virulent strains of Entomopathogenic fungi are effectively formulated and used as bio-insecticides world-wide. Amenable and economical multiplication of a virulent strain in a large scale is important for them to be useful in the field. Culture media plays a major role in the large-scale multiplication of virulent strains of Entomopathogens. Different substrates and media components are being used for this purpose. Yet, each strain differs in its nutritional requirements for the maximum growth and hence it is necessary to standardize the right components and their optimum concentrations in the culture media for a given strain of Entomopathogen. In the current study, three different nitrogen sources and two different carbon sources were tried to standardize the mass multiplication media for seven test isolates of Entomopathogenic fungi. A study was also conducted to determine the ideal grain media for the optimum conidial yields of the test isolates. Yeast extract was found to be the best Nitrogen source for the isolates. The isolates tested, differed in their nutritional requirements and showed variation in the best nitrogen and carbon sources necessary for their growth. Variation was also found in the optimum concentration of both the ingredients for the growth and sporulation of the isolates. In the solid-state fermentation study, rice was found to be the best grain for the growth of most of the fungi followed by barley. The significance of such a study in the development of an effective Myco-insecticide is vital and can be successfully employed in agriculture is discussed.


1971 ◽  
Vol 24 ◽  
Author(s):  
W. H. Verstraete

Some  factors affecting the L-asparaginase activity of E.  aroideae were investigated. Increasing  concentrations of glucose in the culture medium had an inhibiting effect on  the production of L-asparaginase by this microorganism. Buffering of the  culture medium in order to stabilize the pH during growth resulted in a decrease  of the L-asparaginase activity. From the different nitrogen sources examined,  tryptone, proteose peptone nr 2 and nr 3 stimulated the L-asparaginase  production. Toluene treatment of the cells practically destroyed the  L-asparaginase. Acetone dried cells showed an L-asparaginase activity  comparable with the activity of living cells.


1975 ◽  
Vol 28 (3) ◽  
pp. 301 ◽  
Author(s):  
MJ Hynes

Mutants of Apergillus nidulanswith lesions in a gene, areA (formerly called amdT), have been isolated by a variety of different selection methods. The areA mutants show a range of pleiotropic growth responses to a number of compounds as sole nitrogen sources, but are normal in utilization of carbon sources. The levels of two amidase enzymes as well as urease have been investigated in the mutants and have been shown to be affected by this gene. Most of the areA mutants have much lower amidase-specific activities when grown in ammonium-containing medium, compared with mycelium incubated in medium la9king a nitrogen source. Some of the areA. mutants do not show derepression of urease upon relief of ammonium repression. The dominance relationships of areA alleles have been investigated in� heterozygous diploids, and these studies lend support to the proposal that areA codes for a positively acting regulatory product. One of the new areA alleles is partially dominant to areA + and areA102. This may be a result of negative complementation or indicate that areA has an additional negative reiuIatory function. Investigation.of various amdR; areA double mutants has led to the conclusion that amdR and areA participate in independent regulatory circuits in the control of acetamide utilizatiol1. Studies on an amdRc; areA.double mutant indicate that areA is involved in derepression of acetamidase upon relief of ammo.nium repression.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miu Ito ◽  
Yuichi Sugai

AbstractThe effect of nanobubbles on anaerobic growth and metabolism of Pseudomonas aeruginosa was investigated. P. aeruginosa grew earlier in the culture medium containing nanobubbles and the bacterial cell concentration in that culture medium was increased a few times higher compared to the medium without nanobubbles under anaerobic condition. Both gas and protein, which are the metabolites of P. aeruginosa, were remarkably produced in the culture medium containing nanobubbles whereas those metabolites were little detected in the medium without nanobubbles, indicating nanobubbles activated anaerobic growth and metabolism of P. aeruginosa. The carbon dioxide nanobubbles came to be positively charged by adsorbing cations and delivered ferrous ions, one of the trace essential elements for bacterial growth, to the microbial cells, which activated the growth and metabolism of P. aeruginosa. The oxygen nanobubbles activated the activities of P. aeruginosa as an oxygen source.


2006 ◽  
Vol 188 (2) ◽  
pp. 556-568 ◽  
Author(s):  
Biju Joseph ◽  
Karin Przybilla ◽  
Claudia Stühler ◽  
Kristina Schauer ◽  
Jörg Slaghuis ◽  
...  

ABSTRACT A successful transition of Listeria monocytogenes from the extracellular to the intracellular environment requires a precise adaptation response to conditions encountered in the host milieu. Although many key steps in the intracellular lifestyle of this gram-positive pathogen are well characterized, our knowledge about the factors required for cytosolic proliferation is still rather limited. We used DNA microarray and real-time reverse transcriptase PCR analyses to investigate the transcriptional profile of intracellular L. monocytogenes following epithelial cell infection. Approximately 19% of the genes were differentially expressed by at least 1.6-fold relative to their level of transcription when grown in brain heart infusion medium, including genes encoding transporter proteins essential for the uptake of carbon and nitrogen sources, factors involved in anabolic pathways, stress proteins, transcriptional regulators, and proteins of unknown function. To validate the biological relevance of the intracellular gene expression profile, a random mutant library of L. monocytogenes was constructed by insertion-duplication mutagenesis and screened for intracellular-growth-deficient strains. By interfacing the results of both approaches, we provide evidence that L. monocytogenes can use alternative carbon sources like phosphorylated glucose and glycerol and nitrogen sources like ethanolamine during replication in epithelial cells and that the pentose phosphate cycle, but not glycolysis, is the predominant pathway of sugar metabolism in the host environment. Additionally, we show that the synthesis of arginine, isoleucine, leucine, and valine, as well as a species-specific phosphoenolpyruvate-dependent phosphotransferase system, play a major role in the intracellular growth of L. monocytogenes.


Toxins ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 519 ◽  
Author(s):  
Kimiko Yabe ◽  
Haruna Ozaki ◽  
Takuya Maruyama ◽  
Keisuke Hayashi ◽  
Yuki Matto ◽  
...  

The dichlorvos-ammonia (DV-AM) method is a simple but sensitive visual method for detecting aflatoxigenic fungi. Here we sought to develop a selective medium that is appropriate for the growth of aflatoxigenic fungi among soil mycoflora. We examined the effects of different concentrations of carbon sources (sucrose and glucose) and detergents (deoxycholate (DOC), Triton X-100, and Tween 80) on microorganisms in soils, using agar medium supplemented with chloramphenicol. The results demonstrated that 5–10% sucrose concentrations and 0.1–0.15% DOC concentrations were appropriate for the selective detection of aflatoxigenic fungi in soil. We also identified the optimal constituents of the medium on which the normal rapid growth of Rhizopus sp. was completely inhibited. By using the new medium along with the DV-AM method, we succeeded in the isolation of aflatoxigenic fungi from non-agricultural fields in Fukui city, Japan. The fungi were identified as Aspergillus nomius based on their calmodulin gene sequences. These results indicate that the new medium will be useful in practice for the detection of aflatoxigenic fungi in soil samples including those from non-agricultural environments.


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