Development of a Sperm Cryopreservation Protocol for Critically Endangered Mohashol, Tor tor (Hamilton)

2022 ◽  
Author(s):  
M. Salah Uddin Kabir ◽  
M. Rafiqul Islam Sarder ◽  
M. Matiur Rahman ◽  
M. Fazlul Awal Mollah ◽  
N. Binte Ryhan
Keyword(s):  
Critically Endangered ◽  
Sperm Cryopreservation ◽  
Cryopreservation Protocol ◽  
Tor Tor

scholarly journals Effects of Cryoprotective Medium Composition, Dilution Ratio, and Freezing Rates on Spotted Halibut (Verasper variegatus) Sperm Cryopreservation

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2153
Author(s):  
Irfan Zidni ◽  
Yun Ho Lee ◽  
Jung Yeol Park ◽  
Hyo Bin Lee ◽  
Jun Wook Hur ◽  
...  
Keyword(s):  
Previous Experiment ◽  
Activity Index ◽  
Medium Composition ◽  
Freezing Rate ◽  
Dilution Ratio ◽  
Sperm Cryopreservation ◽  
Ringer’S Solution ◽  
Potential Market ◽  
Spotted Halibut ◽  
Cryopreservation Protocol

The spotted halibut is species that has a high potential market value in Korea, but the supply of seed is unstable because of the limited milt production of males. The objective of this research was to explore different aspects, such as CPAs, diluents, dilution ratio, and freezing rates, to develop an optimal sperm cryopreservation. The parameters assessed were movable sperm ratio, sperm activity index, survival rate, and DNA damage. The CPAs tested in this research were propylene glycol, dimethyl sulfoxide (DMSO), methanol, ethylene glycol, and glycerol. Different diluents, including 300 mM sucrose, 300 mM glucose, Stain’s solution, and Ringer’s solution, were investigated. The previous experiment showed that the optimal CPA for cryopreservation was DMSO with a concentration of 15% with 300 mM as diluent. To determine the effect of the dilution ratio, sperm was diluted to 1:1, 1:2, 1:10, 1:100, and 1:1000 with 300 mM sucrose containing DMSO at a final concentration of 15%. Lastly, the optimal freezing rate of the sperm was evaluated with four different freezing rates (−1, −5, −10, and −20 °C/min). Post-thaw sperm motility was higher with a dilution ratio lower than 1:2, and the freezing rate was less than −5 °C/min. In conclusion, these findings represent the development of a cryopreservation protocol for spotted halibut.

scholarly journals Acrosomal integrity and capacitation are not influenced by sperm cryopreservation in the giant panda

Reproduction ◽  
2004 ◽  
Vol 127 (5) ◽  
pp. 547-556 ◽  
Author(s):  
R E Spindler ◽  
Y Huang ◽  
J G Howard ◽  
P Wang ◽  
H Zhang ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Giant Panda ◽  
Calcium Ionophore ◽  
Sperm Cryopreservation ◽  
Management Tools ◽  
Giant Pandas ◽  
Before And After ◽  
Cryopreservation Protocol ◽  
Acrosomal Integrity

Sperm cryopreservation and artificial insemination are important management tools for giant panda breeding and the preservation of extant genetic diversity. This study examined the influence of freeze–thawing on sperm function, specifically capacitation. Sperm from nine giant pandas were assessed before and after rapid (− 40 and − 100 °C/min) cryopreservation by incubation in HEPES-buffered Ham’s F10 medium with and without the capacitation accelerators, 3-isobutyl-1-methylxanthine (IBMX) and dibutyryl cyclic AMP (dbcAMP). At 0, 3 and 6 h of exposure, aliquots were assessed for sperm motility traits and capacitation, defined as the proportion of sperm with intact acrosomes following exposure to solubilised zonae pellucidae (ursid or felid) or calcium ionophore subtracted from the proportion of sperm with intact acrosomes before exposure. Although mean±s.e.m. sperm motility post-thaw (56.1 ± 3.9% at 0 h) was less (P < 0.05) than pre-freeze (71.7 ± 6.0%), there was no difference (P > 0.05) in the proportion of acrosome-intact sperm (fresh, 93.0 ± 1.7% versus cryopreserved–thawed, 81.7 ± 4.7% at 0 h). Incidence of capacitation was greater (P < 0.05) in fresh sperm incubated with capacitation accelerators IBMX and dbcAMP (9 h: 50.9 ± 1.1) compared with fresh sperm incubated without accelerators (9 h: 41.2 ± 1.1%). Frozen–thawed sperm preincubated without accelerators underwent capacitation (49.6 ± 1.1%) to a greater extent (P < 0.05) compared with these fresh counterparts. Thawed samples with (9 h: 45.9 ± 1.4%) and without accelerators (9 h: 41.2 ± 1.1%) did not differ (P > 0.05) during the 9-h incubation. We conclude that giant panda spermatozoa (1) undergo capacitation in vitro with or without chemical accelerators and (2) withstand a rapid cryopreservation protocol, including retaining normal acrosomal integrity and functional capacitation ability.

Development of a sperm cryopreservation protocol for the Argentine black and white tegu (Tupinambis merianae)

2017 ◽  
Vol 87 ◽  
pp. 55-63 ◽  
Author(s):  
Carly Young ◽  
Nicole Ravida ◽  
Michelle Curtis ◽  
Frank Mazzotti ◽  
Barbara Durrant
Keyword(s):  
Sperm Cryopreservation ◽  
Black And White ◽  
Tupinambis Merianae ◽  
Cryopreservation Protocol

Dimethylacetamide can be used as an alternative to glycerol for the successful cryopreservation of koala (Phascolarctos cinereus) spermatozoa

2008 ◽  
Vol 20 (6) ◽  
pp. 724 ◽  
Author(s):  
Yeng Peng Zee ◽  
William V. Holt ◽  
Jaime Gosalvez ◽  
Camryn D. Allen ◽  
Vere Nicolson ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Membrane Integrity ◽  
Sperm Nucleus ◽  
Sperm Chromatin ◽  
Similar Degree ◽  
Sperm Cryopreservation ◽  
Phascolarctos Cinereus ◽  
Degree Of Protection ◽  
Fish Sperm ◽  
Cryopreservation Protocol

Swelling of koala sperm chromatin following cryopreservation has largely been attributed to the absence of intermolecular disulfide cross-linkages in the marsupial sperm nucleus. Fish spermatozoa also lack disulfide bonds within their chromatin, but have been successfully cryopreserved. The present study examined the hypothesis that the cryoprotectants used for fish sperm cryopreservation would confer a similar degree of protection on koala spermatozoa. Three concentrations each of five cryoprotectants (dimethyl sulfoxide, methanol, propylene glycol, ethylene glycol and dimethylacetamide (DMA)) were evaluated. Each treatment was compared against an established koala sperm cryopreservation protocol that uses 14% glycerol. Post-thaw assessment of progressive motility, plasma membrane integrity and mitochondrial membrane potential (MMP) revealed that protocols using 15% DMA achieved 62.2 ± 3.6% (P < 0.05) sperm survival, of which 79% (P < 0.05) had high MMP, an improvement of 32% and 40%, respectively, over sperm frozen in 14% glycerol. The percentage of spermatozoa with swollen nuclei was also lowest when frozen in 15% DMA, both immediately after thawing (18.0 ± 3.5%; P < 0.05) and after 2 h incubation at 35°C (35.8 ± 4.4%; P < 0.05). A second study was conducted to determine the optimal concentration of DMA for use in the cryopreservation of koala spermatozoa. High DMA concentrations (17.5% and 20%) resulted in significantly lower proportions of live spermatozoa showing high MMP immediately after thawing compared with spermatozoa frozen in the lower concentrations. The percentage of koala spermatozoa with swollen chromatin following cryopreservation was not affected by DMA concentration.

Sperm cryopreservation of the critically endangered olive barb (Sarpunti) Puntius sarana (Hamilton, 1822)

Cryobiology ◽  
2011 ◽  
Vol 62 (1) ◽  
pp. 62-67 ◽  
Author(s):  
M. Nahiduzzaman ◽  
M. Mahbubul Hassan ◽  
U. Habiba Khanam ◽  
S.N.A. Mamun ◽  
Mostafa A.R. Hossain ◽  
...  
Keyword(s):  
Critically Endangered ◽  
Sperm Cryopreservation ◽  
Puntius Sarana

Development of sperm cryopreservation protocol of endangered spiny eel, Mastacembelus armatus (Lacepede 1800) for ex-situ conservation

Cryobiology ◽  
2016 ◽  
Vol 73 (3) ◽  
pp. 316-323 ◽  
Author(s):  
M. Mahfujur Rahman ◽  
M. Rahmat Ali ◽  
M. Rafiqul Islam Sarder ◽  
M. Fazlul Awal Mollah ◽  
Najmus Sakib Khan
Keyword(s):  
Ex Situ Conservation ◽  
Ex Situ ◽  
Sperm Cryopreservation ◽  
Mastacembelus Armatus ◽  
Cryopreservation Protocol

scholarly journals Estudios preliminares de las características espermáticas y de criopreservación del coral formador de arrecifes Orbicella faveolata

2018 ◽  
Vol 47 (2) ◽  
Author(s):  
María Juliana Vanegas ◽  
Valeria Pizarro
Keyword(s):  
Sperm Morphology ◽  
Sperm Cryopreservation ◽  
Head Shape ◽  
First Report ◽  
Orbicella Faveolata ◽  
Cryopreservation Protocol ◽  
Mature Spermatozoa ◽  
Broad Scale

Cryopreservation has been recently applied to coral gametes and tissue with successful results that can be applied for different purposes on coral conservation and restoration. In this study, we decided to determine the sperm morphology of the coral Orbicella faveolata and assess the feasibility of sperm cryopreservation using a combination of intracellular (1,2-Propadiol) and extracellular (milk) cryoprotectants, and two frozen treatments for 24 h. Mature spermatozoa had a triangular-like head shape measuring 4.10 ± 0.69 μm(mean ± SD) and long flagellum (43.24 ± 7.99 μm). Fresh sperm remained viable and mobile for more than five hours after being released from the gamete bundles. After cryopreservation, all post-thaw sperm components assessed (morphology, motility and viability) showed no difference in contrast to fresh sperm. This study is the first report of cryopreservation of O. faveolata sperm, however further research is needed to increase the success of the cryopreservation protocol for broad-scale application.

scholarly journals Assessment of semen quality, sperm cryopreservation and heterologous IVF in the critically endangered Iberian lynx (Lynx pardinus)

2009 ◽  
Vol 21 (7) ◽  
pp. 848 ◽  
Author(s):  
Natalia Gañán ◽  
Raquel González ◽  
J. Julián Garde ◽  
Fernando Martínez ◽  
Astrid Vargas ◽  
...  
Keyword(s):  
Semen Quality ◽  
Statistical Significance ◽  
Egg Yolk ◽  
Domestic Cat ◽  
Critically Endangered ◽  
Lynx Lynx ◽  
Sperm Cryopreservation ◽  
Iberian Lynx ◽  
Lynx Pardinus

Semen traits and factors affecting sperm cryopreservation were assessed in the Iberian lynx (Lynx pardinus), a species regarded as the most endangered felid in the world. For cryopreservation, semen was washed, resuspended in a Tes–Tris-based diluent (TEST) or a Tris-based diluent (Biladyl), both with 20% egg yolk and 4% glycerol, loaded into straws, cooled to 5°C using an automated programmable system and frozen on nitrogen vapour. Heterologous IVF of in vitro-matured domestic cat oocytes was used to test the fertilising ability of cryopreserved spermatozoa. Electroejaculates from five males were obtained. Characterisation of the electroejaculates revealed mean (± s.e.m.) values of 3.3 ± 0.6 × 106 total spermatozoa, 73.6 ± 4.6% motile spermatozoa, 23.7 ± 4.0% morphologically normal spermatozoa and 40.7 ± 2.3% spermatozoa with intact acrosomes. After thawing a higher percentage of motile spermatozoa was seen in TEST than in Biladyl (34.0 ± 6.2% v. 7.5 ± 4.8%, respectively; P < 0.05); however, there were no differences in the percentage of intact acrosomes between the two diluents. Iberian lynx spermatozoa fertilised domestic cat oocytes in vitro, with higher fertilisation rates observed for spermatozoa cryopreserved in TEST than in Biladyl, although the difference did not reach statistical significance (20.5 ± 4.5% v. 11.5 ± 6.8%, respectively). There were positive significant relations between the fertilisation rates and both the percentage of normal spermatozoa and the percentage of spermatozoa with an intact acrosome before cryopreservation (P = 0.04). This first report of the collection and cryopreservation of Iberian lynx semen and analysis of fertilising ability is an important step in the development of assisted reproductive techniques for this critically endangered felid species.

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