Long-Term Protection Against Human Papillomavirus E7-Positive Tumor by a Single Vaccination of Adeno-Associated Virus Vectors Encoding a Fusion Protein of Inactivated E7 of Human Papillomavirus 16/18 and Heat Shock Protein 70

2010 ◽  
Vol 21 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Liqiao Zhou ◽  
Tong Zhu ◽  
Xiaojing Ye ◽  
Lin Yang ◽  
Bing Wang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Fusion Protein ◽  
Heat Shock Protein 70 ◽  
Adeno Associated Virus ◽  
Virus Vectors ◽  
Human Papillomavirus 16 ◽  
Single Vaccination

scholarly journals Differential MyD88/IRAK4 requirements for cross-priming and tumor rejection induced by heat shock protein 70-model antigen fusion protein

2012 ◽  
Vol 103 (5) ◽  
pp. 851-859 ◽  
Author(s):  
Shusaku Mizukami ◽  
Chiaki Kajiwara ◽  
Masato Tanaka ◽  
Tsuneyasu Kaisho ◽  
Heiichiro Udono
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Fusion Protein ◽  
Heat Shock Protein 70 ◽  
Tumor Rejection

scholarly journals Recombinant Adeno-Associated Virus Expressing Human Papillomavirus Type 16 E7 Peptide DNA Fused with Heat Shock Protein DNA as a Potential Vaccine for Cervical Cancer

2000 ◽  
Vol 74 (6) ◽  
pp. 2888-2894 ◽  
Author(s):  
Dai-Wei Liu ◽  
Yeou-Ping Tsao ◽  
John T. Kung ◽  
Yu-An Ding ◽  
Huey-Kang Sytwu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Tumor Vaccine ◽  
Peptide Vaccine ◽  
Chimeric Gene ◽  
Adeno Associated Virus ◽  
Potential Vaccine

ABSTRACT In this study, we explore a potential vaccine for human papillomavirus (HPV)-induced tumors, using heat shock protein as an adjuvant, a peptide vaccine for safety, and adeno-associated virus (AAV) as a gene delivery vector. The tumor vaccine was devised by constructing a chimeric gene which contained HPV type 16 E7 cytotoxic T-lymphocyte (CTL) epitope DNA (M. C. Feltkamp, H. L. Smits, M. P. Vierboom, R. P. Minnaar, B. M. de Jongh, J. W. Drijfhout, J. ter Schegget, C. J. Melief, and W. M. Kast, Eur. J. Immunol. 23:2242–2249, 1993) fused with the heat shock protein gene as a tumor vaccine delivered via AAV. Our results demonstrate that this vaccine can eliminate tumor cells in syngeneic animals and induce CD4- and CD8-dependent CTL activity in vitro. Moreover, studies with knockout mice with distinct T-cell deficiencies confirm that CTL-induced tumor protection is CD4 and CD8 dependent. Taken together, the evidence indicates that this chimeric gene delivered by AAV has potential as a cervical cancer vaccine.

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