Paper Industry Wastes as Carbon Sources for Aspergillus Species Cultivation and Production of an Enzymatic Cocktail for Biotechnological Applications

2020 ◽  
Vol 16 (2) ◽  
pp. 56-60
Author(s):  
Thiago M. Pasin ◽  
Ana Sílvia A. Scarcella ◽  
Tássio B. de Oliveira ◽  
Rosymar C. Lucas ◽  
Mariana Cereia ◽  
...  
Keyword(s):  
Paper Industry ◽  
Carbon Sources ◽  
Aspergillus Species ◽  
Biotechnological Applications

Screening for xylanase and β-xylosidase production from wood-inhabiting Penicillium strains for potential use in biotechnological applications

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Jaejung Lee ◽  
Yeongseon Jang ◽  
Hanbyul Lee ◽  
Sangjoon Lee ◽  
Gyu-Hyeok Kim ◽  
...  
Keyword(s):  
Paper Industry ◽  
Carbon Sources ◽  
Xylanase Activity ◽  
Cellulase Activity ◽  
Pulp And Paper Industry ◽  
Biotechnological Applications ◽  
Birchwood Xylan ◽  
Beechwood Xylan ◽  
Filter Paper Assay ◽  
Potential Use

Abstract Experiments were performed to find potential sources for enzyme production for the pulp and paper industry and for biological ethanol production by screening the cellulase, xylanase and β-xylosidase activities of 36 species of Penicillium isolated from various wood materials in Korea. Rice straw powder (RiceP), birchwood xylan (BirchX), and beechwood xylan (BeechX) were supplied as individual carbon sources for the Penicillium species. All Penicillium species tested in this study showed little cellulase activity, but some species exhibited remarkably high xylanase and β-xylosidase activities, as determined by a filter paper assay. P. oxalicum showed the greatest xylanase activity on RiceP (158.70 U ml-1). On the other hand, P. brevicompactum produced the highest active β-xylosidase on BirchX (6.25 U ml-1).

scholarly journals Classification of lipolytic enzymes and their biotechnological applications in the pulping industry

2017 ◽  
Vol 63 (3) ◽  
pp. 179-192 ◽  
Author(s):  
L. Ramnath ◽  
B. Sithole ◽  
R. Govinden
Keyword(s):  
Fatty Acids ◽  
Paper Industry ◽  
Pulp And Paper Industry ◽  
Pulp And Paper ◽  
Biotechnological Applications ◽  
Lipolytic Enzymes ◽  
Prevention And Treatment ◽  
Potential Applications ◽  
Microbial Sources

In the pulp and paper industry, during the manufacturing process, the agglomeration of pitch particles (composed of triglycerides, fatty acids, and esters) leads to the formation of black pitch deposits in the pulp and on machinery, which impacts on the process and pulp quality. Traditional methods of pitch prevention and treatment are no longer feasible due to environmental impact and cost. Consequently, there is a need for more efficient and environmentally friendly approaches. The application of lipolytic enzymes, such as lipases and esterases, could be the sustainable solution to this problem. Therefore, an understanding of their structure, mechanism, and sources are essential. In this report, we review the microbial sources for the different groups of lipolytic enzymes, the differences between lipases and esterases, and their potential applications in the pulping industry.

scholarly journals A Review on Bio Prospective Potential of Corynebacterium glutamicum

2019 ◽  
Vol 7 (4) ◽  
pp. 7-11
Author(s):  
Asfaw Tora Kacho B.S.R. Pattnaik
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Large Scale ◽  
Carbon Sources ◽  
Genetic Alterations ◽  
Industrial Processes ◽  
Biotechnological Applications ◽  
Gram Positive ◽  
Pubmed Database ◽  
Wide Range

Corynebacterium glutamicumis a nonpathogenic, aerobic, gram-positive soil bacterium used for the large-scale biotechnological production of several biomolecules. C. glutamicum is an industrial microbe traditionally used for the production of amino acids. It is a well known microbe as the workhorse in fermentation industry used since its first discovery in Japan in 1950s for production of monosodium glutamate (MSG)and L-lysine with a market size of 3.1 and 2.2 million. However, for the fermentative production of diverse products through genetic–metabolic engineering. Due to its industrial importance, several clones of C. glutamicum have been sequenced by both industry and academic groups. Furthermore it has also been exploited for the synthesis of a variety of other fuels and chemicals. Especially, the facultative anaerobic lifestyle of this Gram-positive bacterium formed the basis to engineer C. glutamicum for the production of reduced molecules such as organic acids (e.g., lactate, succinate) and alcohols (e.g., ethanol, isobutanol) under zero-growth anaerobic conditions. The present work is a review of various uses of C. glutamicum for which the researchers explored several resources including the PubMed database, Journals and online archives upto March 2019. The review in its analysis signifies the vital utility of C. glutamicum in various prospective. Most articles were on occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Through genetic alterations, this microbe showed its efficacy for the simultaneous utilization of hexose and pentose sugars of biomass hydrolysate for making value-added products, such as amino acids and polyamines, and thus making it a strain of choice to work in a bio-refinery concept. This soil microbe also showed its efficacy in bioremediation purposes, such as arsenic removal. Recently its extensive research has focused on engineering beyond the scope of amino acids for utilization of alternative carbon sources, (e.g. coming from wastes and unprocessed sources), and construction of C. glutamicum strains for production of new products such as diamines, organic acids and alcohols. Meanwhile, a variety of corynebacterial strains allows access to alternative carbon sources that allows production of a wide range of industrially relevant compounds. Some of these efforts set new standards in terms of titers and productivities achieved whereas others represent a proof-of-principle. These achievements manifest the position of C. glutamicum as an important industrial microorganism with capabilities far beyond the traditional amino acid production. According to the studies cited in different literatures C. glutamicum reported as the most effective and playing important roles as dominant bacteria in biotechnological applications as well as industrial processes and products. And, with recent advances in biotechnology, the economic contributions that these organisms can make in biotechnological applications and industrial processes can be exploited further for large scale benefit of mankind.

Thermophilic Bacterial Exopolysaccharides

2022 ◽  
pp. 334-361
Author(s):  
Rakesh Goswami ◽  
Bidyut Bandyopadhyay ◽  
Sanjoy Sadhukhan
Keyword(s):  
Hydrothermal Vents ◽  
Extracellular Polymeric Substances ◽  
Hot Spring ◽  
Carbon Sources ◽  
Extreme Environments ◽  
Value Added ◽  
Low Ph ◽  
Extreme Conditions ◽  
Biotechnological Applications ◽  
Bacterial Exopolysaccharides

Bacterial exopolysaccharides have enormous diversity with valuable characteristics, synthesized by various pathways in extreme conditions like salinity, geothermal springs, or hydrothermal vents. Due to extreme environments, these microorganisms have various adaption principles (e.g., low pH, high temperature, high saltation, and high radiation). Exopolysaccharide is an organic compound produced by most bacteria during fermentation using various carbon sources, resulting in a jelly-like or mass network structure outside the cell wall. This biopolymer has an adherent cohesive layer throughout the cell layer. Hot spring bacterial polysaccharides contain diverse extracellular polymeric substances. With a gain in popularity in applications of thermophilic microbial polysaccharides and its demand in diverse value-added industrial products, this chapter aims to provide valuable information on the physicochemical function and biotechnological applications in the field of food, medical imaging, nano-drugs, bioremediation, cancer, anti-bacterial, tissue engineering, etc.

scholarly journals The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation

2007 ◽  
Vol 73 (16) ◽  
pp. 5308-5319 ◽  
Author(s):  
Jesús Caballero-Mellado ◽  
Janette Onofre-Lemus ◽  
Paulina Estrada-de los Santos ◽  
Lourdes Martínez-Aguilar
Keyword(s):  
Aromatic Compounds ◽  
Burkholderia Cepacia ◽  
Growth Promotion ◽  
Carbon Sources ◽  
Acc Deaminase ◽  
Biotechnological Applications ◽  
Environmental Distribution ◽  
Organic Acid Production ◽  
High Level

ABSTRACT Burkholderia strains are promising candidates for biotechnological applications. Unfortunately, most of these strains belong to species of the Burkholderia cepacia complex (Bcc) involved in human infections, hampering potential applications. Novel diazotrophic Burkholderia species, phylogenetically distant from the Bcc species, have been discovered recently, but their environmental distribution and relevant features for agro-biotechnological applications are little known. In this work, the occurrence of N2-fixing Burkholderia species in the rhizospheres and rhizoplanes of tomato plants field grown in Mexico was assessed. The results revealed a high level of diversity of diazotrophic Burkholderia species, including B. unamae, B. xenovorans, B. tropica, and two other unknown species, one of them phylogenetically closely related to B. kururiensis. These N2-fixing Burkholderia species exhibited activities involved in bioremediation, plant growth promotion, or biological control in vitro. Remarkably, B. unamae and B. kururiensis grew with aromatic compounds (phenol and benzene) as carbon sources, and the presence of aromatic oxygenase genes was confirmed in both species. The rhizospheric and endophyte nature of B. unamae and its ability to degrade aromatic compounds suggest that it could be used in rhizoremediation and for improvement of phytoremediation. B. kururiensis and other Burkholderia sp. strains grew with toluene. B. unamae and B. xenovorans exhibited ACC (1-aminocyclopropane-1-carboxylic acid) deaminase activity, and the occurrence of acdS genes encoding ACC deaminase was confirmed. Mineral phosphate solubilization through organic acid production appears to be the mechanism used by most diazotrophic Burkholderia species, but in B. tropica, there presumably exists an additional unknown mechanism. Most of the diazotrophic Burkholderia species produced hydroxamate-type siderophores. Certainly, the N2-fixing Burkholderia species associated with plants have great potential for agro-biotechnological applications.

Polyhydroxyalkanoates (PHA) production using wastewater as carbon source and activated sludge as microorganisms

2006 ◽  
Vol 53 (6) ◽  
pp. 175-180 ◽  
Author(s):  
S. Yan ◽  
R.D. Tyagi ◽  
R.Y. Surampalli
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Volatile Fatty Acids ◽  
Paper Industry ◽  
Carbon Sources ◽  
Dry Weight ◽  
Pulp And Paper Industry ◽  
Pulp And Paper ◽  
Wastewater Sludge ◽  
High Concentration

Activated sludge from different full-scale wastewater treatment plants (municipal, pulp and paper industry, starch manufacturing and cheese manufacturing wastewaters) was used as a source of microorganisms to produce biodegradable plastics in shake flask experiments. Acetate, glucose and different wastewaters were used as carbon sources. Pulp and paper wastewater sludge was found to accumulate maximum concentration (43% of dry weight of suspended solids) of polyhydroxy alkanoates (PHA) with acetate as carbon source. Among the different wastewaters tested as a source of carbon, pulp and paper industry and starch industry wastewaters were found to be the best source of carbon while employing pulp and paper activated sludge for maximum accumulation of PHA. High concentration of volatile fatty acids in these wastewaters was the probable reason.

scholarly journals A Genome-Scale Metabolic Model of 2,3-Butanediol Production by Thermophilic Bacteria Geobacillus icigianus

2020 ◽  
Vol 8 (7) ◽  
pp. 1002
Author(s):  
Mikhail Kulyashov ◽  
Sergey E. Peltek ◽  
Ilya R. Akberdin
Keyword(s):  
Citrate Synthase ◽  
Thermophilic Bacteria ◽  
Carbon Sources ◽  
Metabolic Model ◽  
Biofuel Production ◽  
Biotechnological Applications ◽  
Metabolic Potential ◽  
A Genome ◽  
Wide Range ◽  
Genome Scale

The thermophilic strain of the genus Geobacillus, Geobacillus icigianus is a promising bacterial chassis for a wide range of biotechnological applications. In this study, we explored the metabolic potential of Geobacillus icigianus for the production of 2,3-butanediol (2,3-BTD), one of the cost-effective commodity chemicals. Here we present a genome-scale metabolic model iMK1321 for Geobacillus icigianus constructed using an auto-generating pipeline with consequent thorough manual curation. The model contains 1321 genes and includes 1676 reactions and 1589 metabolites, representing the most-complete and publicly available model of the genus Geobacillus. The developed model provides new insights into thermophilic bacterial metabolism and highlights new strategies for biotechnological applications of the strain. Our analysis suggests that Geobacillus icigianus has a potential for 2,3-butanediol production from a variety of utilized carbon sources, including glycerine, a common byproduct of biofuel production. We identified a set of solutions for enhancing 2,3-BTD production, including cultivation under anaerobic or microaerophilic conditions and decreasing the TCA flux to succinate via reducing citrate synthase activity. Both in silico predicted metabolic alternatives have been previously experimentally verified for closely related strains including the genus Bacillus.

scholarly journals Biotechnological Potential of Agro-Industrial Wastes as a Carbon Source to Thermostable Polygalacturonase Production in Aspergillus niveus

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Alexandre Maller ◽  
André Ricardo Lima Damásio ◽  
Tony Marcio da Silva ◽  
João Atílio Jorge ◽  
Héctor Francisco Terenzi ◽  
...  
Keyword(s):  
Microbial Growth ◽  
Carbon Sources ◽  
Orange Peel ◽  
Industrial Wastes ◽  
Biotechnological Applications ◽  
Biotechnological Potential ◽  
Lemon Peel ◽  
Aspergillus Niveus ◽  
Solid Media ◽  
Polygalacturonase Production

Agro-industrial wastes are mainly composed of complex polysaccharides that might serve as nutrients for microbial growth and production of enzymes. The aim of this work was to study polygalacturonase (PG) production by Aspergillus niveus cultured on liquid or solid media supplemented with agro-industrial wastes. Submerged fermentation (SbmF) was tested using Czapeck media supplemented with 28 different carbon sources. Among these, orange peel was the best PG inducer. On the other hand, for solid state fermentation (SSF), lemon peel was the best inducer. By comparing SbmF with SSF, both supplemented with lemon peel, it was observed that PG levels were 4.4-fold higher under SSF. Maximum PG activity was observed at 55∘C and pH 4.0. The enzyme was stable at 60∘C for 90 min and at pH 3.0–5.0. The properties of this enzyme, produced on inexpensive fermentation substrates, were interesting and suggested several biotechnological applications.

scholarly journals Optimization and Characterization of Biocellulose Production from Bacteria Isolated from Passion Fruits

2021 ◽  
Vol 12 (1S) ◽  
pp. 21-30
Author(s):  
Muhammad Haikal Hizani ◽  
Nadiawati Alias ◽  
Syamil Choo Shaiful Bahri ◽  
Khairul Anuar Apendi
Keyword(s):  
Paper Industry ◽  
Carbon Sources ◽  
Endophytic Bacterium ◽  
Organic Polymer ◽  
Bending Vibration ◽  
Initial Ph ◽  
The Media ◽  
Biomedical Industry ◽  
Intramolecular Hydrogen

Biocellulose is a strong polymer consisting of nanofibrillar structures that produce a large surface area and a microporous structure. This organic polymer is greatly in demand in various industries, such as the paper industry, biomedical industry and cosmetics industry.  In this study, biocellulose production from two bacteria known as endophytic bacterium SV845 (M02) and Pantoea ananatis IADCAMBID (M03) isolated from passion fruit was selected. Three different media formulations (Media 1, Media 2, and Media 3) were used in order to optimize the biocellulose production where each media contained a different percentage of carbon sources (glucose and fructose). The highest biocellulose production (1.544 mg/mL) was demonstrated by M02 bacteria strain in Media 1 containing glucose alone which fermented at 30 ºC while at 37 ºC, the highest biocellulose (BC) production was demonstrated by M03 bacteria strain at 2.078 mg/mL in media containing glucose alone (Media 1). Data on pH changes during biocellulose fermentation in all the media were set at an initial pH of 6. The final pH values were observed in the range of 5.34 to 6.08 for M02 strain and 5.95 to 8.53 for M03 strain, respectively. Characterizations of biocellulose were compared to starch using Fourier Transform Infrared (FTIR) spectroscopy. FTIR analysis indicated that the absorption peaks at 3200 cm-1 and 1630 cm-1 were derived from the association of intermolecular and intramolecular hydrogen bonds and H–O–H bending vibration of the absorbed water molecules in cellulose.

scholarly journals Isolation Of Pectobacterium Carotovorum, Identification With 16s Rrna, Phytase Activity And Characterization Of The Bacteria

2019 ◽  
Author(s):  
Kağan Tolga CİNİSLİ ◽  
Safa Mustafa KILIÇ ◽  
Sevda Uçar ◽  
Emre CANCA ◽  
Neslihan dikbaş
Keyword(s):  
16S Rrna ◽  
Food Industry ◽  
Inositol Phosphates ◽  
Paper Industry ◽  
Pectobacterium Carotovorum ◽  
Biotechnological Applications ◽  
Commercial Use ◽  
Effective Technology ◽  
First Time

Abstract Background: Phytases can be produced by animals, plants and microorganisms. However, the most promising ones for commercial use and biotechnological applications are those of microbial origin. Phytases are also used in the preparation of myo-inositol phosphates in the food industry, soil remediation and in the paper industry. Biotechnology, along with the increased use of phytase enzymes, is a highly effective technology that is used today and will be used in the future to produce these enzymes and improve their properties. Results: The aim of this study was to conduct the molecular identification of Pectobacterium carotovorum strains isolated from lettuce to produce phytase from a new microbial source and the characterization of the enzyme. The activity and characterization of the phytase obtained from the bacterium was carried out. Isolation of strains was carried out following incubation at 26 ° C for 48 hours using Nutrient agar (Oxoid). The identification was performed using the 16S rRNA method. The phytase produced from Pectobacterium carotovorum showed the best activity at pH 8.0. The optimum temperature of the phytase obtained from Pectobacterium carotovorum was 60 ° C. Conclusions: In this study, enzymatic activity of phytase was investigated in Pectobacterium carotovorum for the first time. The results showed that it can be used in the industry due to the characteristics of the enzyme produced by Pectobacterium carotovorum. Keywords: Pectobacterium carotovorum, 16S rRNA, Phytase, Characterization

Sign in / Sign up

Export Citation Format

Share Document