Oxygen plasma modulates glucosinolate levels without affecting lipid contents and composition in Brassica napus seeds

2021 ◽  
Author(s):  
Akiko Maruyama-Nakashita ◽  
Yohei Ishibashi ◽  
Kyotaro Yamamoto ◽  
Zhang Liu ◽  
Tomomi Morikawa-Ichinose ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Plant Breeding ◽  
Lipid Content ◽  
Oxygen Plasma ◽  
Seed Viability ◽  
The South ◽  
Lipid Contents ◽  
Animal Feeding ◽  
Plasma Oxygen

Abstract Rapeseed contains high levels of glucosinolates (GSLs), playing pivotal roles in defense against herbivores and pests. As their presence in rapeseed reduces the value of the meal for animal feeding, intensive efforts to reduce them produced low-seed GSL cultivars. However, there is no such variety suitable for the south part of Japan. Here, we tested the effects of cold oxygen plasma (oxygen CP) on seed germination and GSL and lipid content, in three rapeseed cultivars. According to the cultivars, oxygen CP slightly stimulated seed germination and modified the GSL levels; decreased GSL levels in Westar and Kizakinonatane but increased those in Nanashikibu. In contrast, it negligibly affected the lipid content and composition in the three cultivars. Thus, oxygen CP modulated seed GSL levels without affecting seed viability and lipid content. Future optimization of this technique may help optimize rapeseed GSL content without plant breeding.

Lipid content of honey bee-collected pollen from south-east Australia

2005 ◽  
Vol 45 (12) ◽  
pp. 1659 ◽  
Author(s):  
D. C. Somerville
Keyword(s):  
Brassica Napus ◽  
Solvent Extraction ◽  
Honey Bee ◽  
Lipid Content ◽  
Endemic Species ◽  
Honey Bees ◽  
Lipid Levels ◽  
Lipid Contents ◽  
The Mean ◽  
Rapistrum Rugosum

The use of solvent extraction or petroleum spirits to extract lipids (fats) from 172 samples of honey bee-collected pollens provided a range of lipid contents from 0% for Eucalyptus macrorhyncha to 11.2% for Hypochoeris radicata. The mean for all 172 samples, representing 61 species, was 2.52%. The mean from 31 endemic species was 1.78%, whereas the mean for 30 exotic species was 4.13%. When pollens from 1 species, Echium plantagineum, were extensively surveyed, the lipid levels could be predictably estimated, falling within a range of 0.6 to 2.46% and a mean of 1.6%. A number of pollens from particular species consistently showed high levels of lipids, which were observed to be highly favoured by foraging honey bees. The pollens noted to be particularly attractive to foraging honey bees included Brassica napus (mean 7.1%), Sisymbrium officinale (mean 5.8%), Rapistrum rugosum (mean 6%) and Hypochoeris radicata (mean 7.2%).

scholarly journals Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

2021 ◽  
Author(s):  
Filippo Guzzon ◽  
Maraeva Gianella ◽  
Jose Alejandro Velazquez Juarez ◽  
Cesar Sanchez Cano ◽  
Denise E Costich
Keyword(s):  
Seed Germination ◽  
Plant Genetic Resources ◽  
Seed Viability ◽  
Storage Conditions ◽  
Seed Longevity ◽  
Germplasm Bank ◽  
Significant Difference ◽  
Different Temperatures ◽  
Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

scholarly journals Using Microwave Soil Heating to Inhibit Invasive Species Seed Germination

2017 ◽  
Vol 10 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Mélissa De Wilde ◽  
Elise Buisson ◽  
Nicole Yavercovski ◽  
Loïc Willm ◽  
Livia Bieder ◽  
...  
Keyword(s):  
Invasive Species ◽  
Soil Moisture ◽  
Seed Germination ◽  
Invasive Plant ◽  
Organic Matter Content ◽  
Seed Viability ◽  
Germination Percentage ◽  
Matter Content ◽  
Burial Depth ◽  
Experimental Conditions

Successful invasive plant eradication is rare, because the methods used target the adult stage, not taking into account the development capacity of a large seedbank. Heating by microwave was considered, because it offers a means to quickly reach the temperature required for loss of seed viability and inhibition of germination. Previous results were not encouraging, because homogeneous and deep-wave penetration was not achieved, and the various parameters that can affect treatment effectiveness were incompletely addressed. This study aimed to determine, under experimental conditions, the best microwave treatment to inhibit invasive species seed germination in terms of power (2, 4, 6 kW) and duration (2, 4, 8 min) of treatments and depending on soil moisture (10%, 13%, 20%, 30%) and seed burial depth (2, 12 cm). Three invasive species were tested: Bohemian knotweed, giant goldenrod, and jimsonweed. The most effective treatments required relatively high power and duration (2kW8min, 4kW4min, 6kW2min, and 6kW4min; 4kW8min and 6kW8min were not tested for technical reasons), and their effectiveness diminished with increasing soil moisture with germination percentage between 0% and 2% for the lowest soil moisture, 0% and 56% for intermediate soil moisture, and 27% and 68% in control treatments. For the highest soil moisture, only 2kW8min and 4kW4min reduced germination percentage between 2% and 19%. Occasionally, germination of seeds located at the 12-cm depth was more strongly affected. Giant goldenrod seeds were the most sensitive, probably due to their small size. Results are promising and justify further experiments before developing a field microwave device to treat large volumes of soil infested by invasive seed efficiently and with reasonable energy requirements. Other types of soil, in terms of texture and organic matter content, should be tested in future experiments, because these factors influence soil water content and, consequently, microwave heating.

scholarly journals Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 490
Author(s):  
Saeng Geul Baek ◽  
Jin Hyun Im ◽  
Myeong Ja Kwak ◽  
Cho Hee Park ◽  
Mi Hyun Lee ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Seed Viability ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Viability Test ◽  
Physiological Dormancy ◽  
Different Temperatures ◽  
Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

scholarly journals Activation of rape (Brassica napus L.) embryo during seed germination. V. The first zones of ultrastructural changes and their expansion

2014 ◽  
Vol 56 (1) ◽  
pp. 77-91 ◽  
Author(s):  
Mieczysław Karaś
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Seed Coat ◽  
Basal Part ◽  
Ultrastructural Changes ◽  
Embryo Axis ◽  
Brassica Napus L ◽  
Root Cells ◽  
Basal Zone

In the germinating rape embryo the columella and basal part of hypocotyl undergo earliest activation. Its first ultrastructural symptom is the appearance of numerous ER vesicles after 3-6 h of seed swelling. Their number is the highest in the external layers of the columella and decreases in basipetal direction. Dermatogen cells in the basal zone of the hypocotyl contain the greatest amount of ER structures, whereas decreasing amounts are found in both directions along the embryo axis and centripetally. Further changes in the ER spread in a similar order. The vesicles merge and form a tubular and plate-like ER. Then, they disappear and are replaced by tubular and vesicular forms. The changes in the ER are gradually followed by ultrastructural symptoms of activation of mitochondria, plastids and dictyosomes. The highest number of ER structures and other organelles accumulate in root cells shortly before piercing of the seed coat. After germination their amount decreases and remains almost stable.

scholarly journals Flowering, Fecundity, Seed Germination, and Seed Viability of Viburnum opulus L. Cultivars

2009 ◽  
Vol 27 (1) ◽  
pp. 31-36
Author(s):  
Janine R. Conklin ◽  
James C. Sellmer
Keyword(s):  
Seed Germination ◽  
Seed Production ◽  
Seed Viability ◽  
Biological Traits ◽  
Limited Information ◽  
Viable Seed ◽  
Invasive Potential ◽  
Short Term ◽  
Viburnum Opulus ◽  
Tetrazolium Test

Abstract Mature specimens of Viburnum opulus and cultivars ‘Leonard's Dwarf’ and ‘Roseum’ were assessed over 2 years for flower and seed production, seed germination, and seed viability as determined by a tetrazolium test to understand their invasive potential. ‘Aureum’, ‘Compactum’, ‘Losely's Compact’, ‘Nanum’, and ‘Xanthocarpum’ were also tested for germination and viability of seeds. Cultivars differed in flower and seed production, seed germination, and seed viability. ‘Roseum’ prolifically produced highly viable seed that germinated at moderate rates under greenhouse conditions (8,354, 100%, and 73%, respectively). Viburnum opulus and ‘Leonard's Dwarf’ produced fewer viable seed which showed moderate to low germination rates (609, 100%, and 53%; 712, 100%, and 5%, respectively). ‘Aureum’ and ‘Xanthocarpum’ seeds germinated at moderate rates (55 and 25%, respectively) and were highly viable (100%). ‘Compactum’, ‘Losely's Compact’, and ‘Nanum’ germinated at low rates or failed to germinate (0, 0, and 5%, respectively), yet seeds were moderately viable (37, 65, and 55%, respectively). Seeds of all cultivars germinated at low rates or failed to germinate at both outdoor sites (0 to 5%) which suggests these plants may be weakly invasive. Short-term studies on biological traits such as these provide only limited information to assess the invasive potential of cultivars.

Indopiptadenia Oudhensis (Brandis) Brenan an Endangered Tree Species: Measures for the Coservation

2014 ◽  
Vol 37 (1) ◽  
pp. 69-72
Author(s):  
Giriraj Panwar ◽  
Kumar Ambrish ◽  
S. Srivastava
Keyword(s):  
Seed Germination ◽  
Tree Species ◽  
Seed Viability ◽  
Habitat Destruction ◽  
Anthropogenic Pressure ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Poor Seed ◽  
Over Exploitation

Indopiptadenia oudhensis (Brandis) Brenan is an endangered tree species of family Mimosaceae. Species is mainly distributed at Indo-Nepal border and facing threats such as anthropogenic pressure, habitat destruction, over exploitation, low seed viability and poor seed germination.

scholarly journals Seed germination at different stratification temperatures and development of Phytelephas macrocarpa Ruiz & Pavón seedlings

2017 ◽  
Vol 39 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Sidney Alberto do Nascimento Ferreira ◽  
Daniel Felipe de Oliveira Gentil
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seed Viability ◽  
Seedling Development ◽  
Development Stages ◽  
Plastic Bags ◽  
Alternating Temperature

Abstract: Phytelephas macrocarpa (ivory palm) is an Amazonian palm vulnerable to exploitation pressure, as its seeds are widely used in regional handicrafts. The aims of this study were to evaluate the effectiveness of different stratification temperatures in overcoming seed dormancy and to analyze the seedling development stages of this species. In germination under stratification, the seeds were placed in plastic bags containing moistened vermiculite, and maintained at the constant temperatures of 25, 30, 35 and 40 °C, and an alternating temperature from 26 to 40 °C. In the study about the development of seedlings, seeds were sown in vermiculite under plastic cover (growing house), and the evolution of the seedling was evaluated, from the formation of germinative button to the complete blade expansion of the first eophyll. Stratification at alternating temperatures (26 to 40 °C) helped overcoming seed dormancy. Stratification at 25 °C kept the seed viability for nine months. Germination, characterized by the formation of the germinative button took an average of 114 ± 24 days, and the seedling development until the first extended eophyll lasted 244 ± 57 days.

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