Allozyme heterozygosity and growth in the tiger salamander, Ambystoma tigrinum

1982 ◽  
Vol 73 (4) ◽  
pp. 250-253 ◽  
Author(s):  
Benjamin A. Pierce ◽  
Jeffry B. Mitton
Keyword(s):  
Ambystoma Tigrinum ◽  
Tiger Salamander

scholarly journals Short-term storage of tiger salamander (Ambystoma tigrinum) spermatozoa: The effect of collection type, temperature and time

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245047
Author(s):  
Amanda B. Gillis ◽  
Emmet L. Guy ◽  
Andrew J. Kouba ◽  
Peter J. Allen ◽  
Ruth M. Marcec-Greaves ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Hormone Treatment ◽  
Effect Of Temperature ◽  
Initial Assessment ◽  
Ambystoma Tigrinum ◽  
Tiger Salamander ◽  
Storage Duration ◽  
Spermatozoa Motility ◽  
Over Time

The aims of this project were to characterize tiger salamander (Ambystoma tigrinum) spermatozoa motility over time, when excreted as either milt or spermic urine prior to packaging into a spermatophore, and to determine the effect of temperature on sperm motility. A split-plot design was utilized to assess the motility of the two pre-spermatophore sample types at two temperatures, 0°C and 20°C (n = 10 for each treatment). Spermiation was induced through exogenous hormone treatment of luteinizing hormone releasing hormone analog in order to collect both milt and spermic urine, which were evaluated for motility, divided into two separate aliquots, and subsequently stored in either an ice-bath (0°C) or on the benchtop (20°C). The decay rate of sperm motility was assessed by reevaluating subsamples at 0.5, 1, 2, 3, 5, 7, and 24 hours following the initial assessment. Results showed that sperm stored at 0°C had significantly higher progressive, non-progressive, and total motility for both sperm collection types over time. An interaction was found between collection type and time, with milt exhibiting lower initial motility that was more sustainable over time, compared to spermic urine. For both milt and spermic urine, motility decreased rapidly with storage duration, indicating samples should be used as soon as possible to maximize motility for in-vitro fertilization and cryopreservation. This is the first study to describe the differences in sperm motility between milt and spermic urine from an internally fertilizing caudate and demonstrates the benefits of near freezing temperatures on sperm longevity.

Microsatellite mutation rates in the eastern tiger salamander (Ambystoma tigrinum tigrinum) differ 10-fold across loci

Genetica ◽  
2008 ◽  
Vol 136 (3) ◽  
pp. 501-504 ◽  
Author(s):  
Zafer Bulut ◽  
Cory R. McCormick ◽  
David Gopurenko ◽  
Rod N. Williams ◽  
David H. Bos ◽  
...  
Keyword(s):  
Mutation Rates ◽  
Ambystoma Tigrinum ◽  
Tiger Salamander ◽  
Microsatellite Mutation ◽  
Ambystoma Tigrinum Tigrinum

Insights into the mating habits of the tiger salamander (Ambystoma tigrinum tigrinum) as revealed by genetic parentage analyses

2006 ◽  
Vol 15 (7) ◽  
pp. 1917-1928 ◽  
Author(s):  
DAVID GOPURENKO ◽  
ROD N. WILLIAMS ◽  
CORY R. McCORMICK ◽  
J. ANDREW DeWOODY
Keyword(s):  
Ambystoma Tigrinum ◽  
Tiger Salamander ◽  
Ambystoma Tigrinum Tigrinum
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