Identification of Malassezia Species in Seborrheic Dermatitis Patients by CHROM Agar-Medium

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mohamed Abd Alnaeem Sallam ◽  
Mohamed Taha Mahmoud El-sayed ◽  
Ahmed Abd Elfattah Afify ◽  
Mustafa Nooruldeen Abdulqader Mustafa

Abstract Background Seborrheic dermatitis (SD) is a subacute or chronic superficial inflammatory skin condition, characterized by pruritic, erythematous plaques with greasy, yellow-gray scales, which appear on areas rich in sebaceous glands such as the face, scalp, upper chest, and back. Aim of the Work To identify Malassezia species that are involved in the development of SD in different body sites (face, scalp and trunk) and in different age groups, by using Chrom agar. Patients and Methods Forty nine patients with seborrheic dermatitis attending dermatology outpatient clinic in Ain shams university hospital the samples collected from December 2018 to June 2019. Results In this study, the most affected areas were the scalp and other sites as face and ears, which is concordant with the majority of studies worldwide. The role of sex in propensity to the development of Malassezia spp. Infection in SD is still unclear. Conclusion The distribution of Malassezia spp. on healthy and diseased skin shows significant variation and differences, although our knowledge of Malassezia yeasts has increased tremendously during the last two decades, their pathological roles remain ambiguous, and there is currently no conclusive evidence that any given species is responsible for a specific disease.

2021 ◽  
Vol 108 (Supplement_6) ◽  
Author(s):  
L Coady ◽  
A Charturvedi ◽  
N Fearon ◽  
H Heneghan ◽  
E Carrington

Abstract Background COVID-19 has profoundly impacted the healthcare education environment and telemedicine has been used to augment face-to-face learning and support social-distancing. There is little evidence which examines how patients feel about participating in teaching with this technology. Method A prospective survey of 229 surgical inpatients/outpatients was conducted at a university hospital between August–September 2020. The survey consisted of 31 questions (7-point Likert scales, quantitative items, and free-text answers) to explore: general attitudes towards teaching, attitudes towards COVID-19, perceptions of face-to-face teaching and perceptions of telemedicine teaching. Relationships between demographics and areas of interest were analysed qualitatively and quantitatively. Results Patients reported a positive attitude towards both face-to-face (mean 5.5 [1.1SD]) and telemedicine teaching (5.4 [1.2]) however there was a preference for the face-to-face approach (mean difference [MD]=.16, p = 0.14). Face-to-face teaching was found to be less bothersome (MD=.32, p=.002) and more rewarding (MD.33, p<.001). Older patients were more likely to report both teaching techniques as embarrassing (face-to-face r=.16, p=.048; telemedicine r=.15, p=.036) or as an infringement on their privacy (face-to-face r=.13, p=.048; telemedicine r=.17, p=.017). Qualitative analyses revealed four themes of the patient experience: apprentice role of students, communication skills, transient need for telemedicine during COVID-19 and privacy concerns. Conclusions Despite reporting a favourable impression of telemedicine teaching, patients prefer traditional face-to-face styles as they find telemedicine more bothersome and less rewarding. Educators should take this into consideration during planning of teaching during the pandemic.


Morphologia ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 35-41
Author(s):  
O. I. Makarchuk ◽  
Yu. V. Silkina ◽  
I. V. Tverdokhlib

Background. Improvement of the technical aspects of surgery in aesthetic surgery led to an increase in the number of transactions and significant increase contingent of women who are turning to specialized clinics. But it also significantly increased the proportion of patients with a variety of concomitant diseases requiring new approaches to operational technologies and special attention in the postoperative period. In this group of patients is high risk of intraoperative and postoperative complications significantly limits the range of indications. So to prevent these complications is an important question when planning operations in aesthetic surgery of the face. Objective. Determine structural and functional changes of temporal area skin in women of different age groups with an additional internal pathology. Methods. Intraoperative biopsy material of skin of 104 women at the age from 19 to 73 years, that was taken during standard surgery instrumentations for different defects of face skin correction, was investigated. Results and conclusion. It was determined, that involutive dynamic of microvessel condition in papillary layer of derma coincides with grade reduction of relative volume of microvessels bed. Microcirculation age changes include structural disorders of intrapapillary capillary loops, disorganization of arterioles in papillary and reticular layers of derma, disorders of venules because of the changes in microenvironmental fibrillar network. It is typical at the patients with nicotinic dependence, ischemic heart disease, hypertonic disease, a diabetes, and also adiposity of a different degree essential infringement of microvessels bed structure criteria of skin condition that gives the basis for allocation of the given contingent of patients as group of high intraoperative and postoperative risk at carrying out of frontlift.


2019 ◽  
Vol 63 (4) ◽  
pp. 54-59
Author(s):  
Z. Malinovská ◽  
E. Čonková ◽  
P. Váczi ◽  
M. Harčárová ◽  
E. Böhmová

Abstract The genus Malassezia currently includes seventeen species that have been isolated from healthy and diseased human and other animal skin. Malassezia are implicated in a range of cutaneous diseases in humans: pityriasis versicolor, atopic or seborrheic dermatitis, dandruff, folliculitis and psoriasis. The outbreak of the disease depends on the interaction between the host immune system and Malassezia species. Malassezia stimulates both the cellular and humoral immune response in humans. Although Malassezia species have been associated with various dermatological diseases in people, the detailed pathological role of Malassezia remains obscured. Malassezia yeasts require lipids for their growth and therefore to a greater extent they colonize the sites with more sebaceous glands. The ecosystem on skin is complex and its balance depends on several factors. The aim of this study was to determine the presence of Malassezia yeasts in clinically normal skin of 42 healthy, randomly selected individuals of different ages. In the group of people examined, up to 30 persons (71.4 %) represented by children, adults and the elderly were positive to Malassezia yeasts. It has been shown that the back is an area with a higher incidence (66.7 %) of observed yeast compared to the head (40.5 %).


Author(s):  
SURAJKUMAR ARUN BANGAR

Background: Psoriasis is a non-infectious chronic inflammatory disease of skin characterized by well-defined erythematous plaques with silvery scales having chronic fluctuatory course affecting both sex and different age groups. In India, its prevalence varies from 0.84% - 6%. Psoriasis can be correlated to different varieties of (Skin diseases) like or due to the resemblances of signs and symptoms. The etiological factors explained for can be classified as and. Each plays an important role in the manifestation of the disease. As Ayurveda emphasizes as the first line of treatment to treat a disease, so it is essential to prevent the manifestation of the disease and further to check the progression of the disease through and which the unique contributions of Ayurvedic science are. Aim & Obective: The objective of this review is to assess dietary factors which play a role in psoriasis. Discussion: The risk of dietary factors & their interactions in psoriasis is discussed. Conclusion: In this review, dietary factors which play a role in psoriasis are assessed and their potential benefit is evaluated. Keyword:  Psoriasis, Kushtha, Pathya, Apathya, Ahara Vihara, Sadvritta.


2016 ◽  
Vol 6 (2) ◽  
pp. 96-111
Author(s):  
Maija Butters

In my ethnographic research on death and dying in contemporary Finland, I explore how Finns facing end of life due to a long-term illness or other terminal condition seek to orient themselves and make meaning with cultural tools such as imagery, language, and metaphysical thinking. My primary research material is based on extensive fieldwork at Terhokoti Hospice and in the cancer clinic of Helsinki University Hospital, where I have had numerous conversations with terminally ill patients. This paper seeks to explore the way in which metaphysical aesthetics is assuming the role that religious thinking has traditionally played. When the role of institutional religion is diminishing, it becomes important to understand how emotional and spiritual resolution can be arrived at by means of aesthetics.


Author(s):  
Elaheh Mahmoudi ◽  
Jahangir Rezaie

Background and Purpose: Seborrheic dermatitis (SD) is characterized by erythematous inflammatory patches that mostly appear in the sebaceous gland-rich skin areas. In addition to the key role of Malassezia species in SD, its contribution to other fungal microbiota has been recently addressed in the literature. Regarding this, the present study was conducted to identify and determine the fungal species associated with the incidence of SD. Materials and Methods: For the purpose of the study, fungal microbiome in scaling samples were collected from SD lesions and then analyzed based on the DNA sequencing of ITS regions. Results: In addition to Malassezia, several fungal species were detected in the samples collected from the SD lesions. According to the results, 15.5%, 13.3%, and 6.7% of the isolates were identified as Candida parapsilosis, Cryptococcus albidus var. albidus/ Rhodotorula mucilaginosa, and Penicillium polonicum, respectively. Conclusion: Based on the obtained results, C. parapsilosis was the most prevalent non-Malassezia species isolated from SD lesions. Our results provided basic information about a specific fungal population accounting for the incidence of SD.


2021 ◽  
Vol 97 (3) ◽  
pp. 73-79
Author(s):  
Anna L. Evseeva ◽  
Vera V. Ryabova ◽  
Sergei V. Koshkin

The article presents a clinical case of papulopustular rosacea from our own practice. First of all, the patient's anamnesis is extremely interesting: family history (his brother has episodes of reddening of the face), the onset of rosacea against the background of a stressful situation, the presence of gastric diseases, the connection with nutritional factors. One of the first mentions of rosacea belongs to Avicenna, who in his writings described the clinical picture of rhinophyma ("badschenan", later renamed "abedsamen"). The pineal nose, dotted with "serpentine" vessels, has traditionally been associated with excessive drinking. Currently, the role of alcohol as an etiological factor is not paramount, but its aggravating effect has been proven. Despite the typical picture of rosacea, our patient has been receiving therapy for seborrheic dermatitis and other diseases several times over the course of several years. Probably, the specialists had doubts about the possible diagnosis of rosacea in a male patient under the age of 30.


2019 ◽  
Vol 7 (3) ◽  
pp. 369-372
Author(s):  
Svetlan Dermendzhiev ◽  
Atanaska Petrova ◽  
Tihomir Dermendzhiev

BACKGROUND: Angioneurotic oedema (AE) is an unpredictable and dangerous disease directly threatening the patient's life due to a sudden onset of upper respiratory tract obstruction. The disease is associated with various causes and triggering factors, but little is known about the conditions that accompany AE. AIM: The study aims to determine the age-specificities and the spectrum of concomitant diseases in patients with AE. MATERIAL AND METHODS: The subjects of observation were 88 patients (53 women and 35 men) with angioneurotic oedema who underwent diagnostics and treatment in the Department of Occupational Diseases and Clinical Allergology of University hospital “Saint George”-Plovdiv. RESULTS: The highest level of disease prevalence was found in the age group over 50 years, both in males (45.71%) and females (54.72%). We found that the most often concomitant diseases in our patients with AE are cardiovascular (33%). On second place are the patients with other accompanying conditions outside of the target groups (27.3%). Patients with AE and autoimmune thyroiditis were 14.8%, and those with AE and skeletal-muscle disorders-10.2%. Given the role of hereditary factors in this disease, the profession of the patients is considered insignificant. CONCLUSION: Angioedema occurs in all age groups, but half of the cases are in people over 50 years of age. The most common concomitant diseases in angioedema are cardiovascular diseases.


2021 ◽  
pp. 29-32
Author(s):  
E. M. Markelova

Seborrheic dermatitis (SD) is a chronic recurrent inflammatory skin disease caused by changes of quantitative and qualitative sebum characteristics. SD comes out by appearing of erythematous plaques with sense of itch and peeling skin in areas with high concentration of sebaceous glands: on the scalp, face, upper part of the body and in the folds of skin. Due to the fact that the development of the SD is promoted by the colonization of the skin with a lipophilic yeast fungus Malassezia spp., in the treatment of uncomplicated simple forms of the disease can be applied external antifungal medicines, topical glucocorticosteroids and anti-inflammatory drugs of non-steroidal origin. Systemic therapy is also used in severe course of seborrheic dermatitis and in the treatment of forms, resistant to external therapy. In the treatment of persistent and long-term forms of SD, systemic antifungal agents and systemic retinoids are used, which allow for a long time to achieve regression of skin rashes and significantly improve the quality of life of the patient.


2019 ◽  
Vol 22 (2) ◽  
pp. 146-152
Author(s):  
Kohinur Begum ◽  
Farjahan Nur ◽  
Mashmum Shababa Shahid

Dandruff is a perennial skin condition manifesting as inflammation and physiologic scaling on the scalp. Malassezia species are the part of the skin microflora of humans and the etiological agents of dandruff. Malassezia comprises of lipophilic and non-lipophilic yeasts. At present topical azoles are used for the treatment of dandruff. This study was conducted to isolate and identify the Malassezia spp. from dandruff samples and to determine their responsiveness towards antifungal agents such as fluconazole, ketoconazole, miconazole and nystatin. Malassezia spp. was isolated using selective media and identified by biochemical tests and microscopic examination. Antifungal activities were assessed by the Kirby Bauer method using well diffusion technique. Total fifteen Malassezia spp. including M. furfur, M. pachydermatis, M. sympodialis and M. globosa were identified from twenty five dandruff samples. Among them, M. furfur was most prevalent (66.67%) followed by M. pachydermatis (13.33%), M. sympodialis (13.33%) and M. globosa (6.67%). All strains were sensitive to the antifungal agents but to different extent. Fluconazole and ketoconazole proved to be the most effective agents against these microorganisms. Bangladesh Pharmaceutical Journal 22(2): 146-152, 2019


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