Gelatin methacrylate hydrogel scaffold carrying resveratrol-loaded solid lipid nanoparticles for enhancement of osteogenic differentiation of BMSCs and effective bone regeneration

2021 ◽  
Bangguo Wei ◽  
Wenrui Wang ◽  
Xiangyu Liu ◽  
Chenxi Xu ◽  
Yanan Wang ◽  

Abstract Critical-sized bone defects caused by traumatic fractures, tumour resection, and congenital malformation are unlikely to heal spontaneously. Bone tissue engineering is a promising strategy aimed at developing in vitro replacements for bone transplantation and overcoming the limitations of natural bone grafts. In this study, we developed an innovative bone engineering scaffold based on gelatin methacrylate (GelMA) hydrogel, obtained via a two-step procedure: first, solid lipid nanoparticles (SLNs) were loaded with resveratrol (Res), a drug that can promote osteogenic differentiation and bone formation; these particles were then encapsulated at different concentrations (0.01%, 0.02%, 0.04%, and 0.08%) in GelMA to obtain the final Res-SLNs/GelMA scaffolds. The effects of these scaffolds on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and bone regeneration in rat cranial defects were evaluated using various characterization assays. Our in vitro and in vivo investigations demonstrated that the different Res-SLNs/GelMA scaffolds improved the osteogenic differentiation of BMSCs, with the ideally slow and steady release of Res; the optimal scaffold was 0.02 Res-SLNs/GelMA. Therefore, the 0.02 Res-SLNs/GelMA hydrogel is an appropriate release system for Res with good biocompatibility, osteoconduction, and osteoinduction, thereby showing potential for application in bone tissue engineering.

2019 ◽  
Vol 7 (4) ◽  
pp. 1565-1573 ◽  
Xiao-Yuan Peng ◽  
Min Hu ◽  
Fang Liao ◽  
Fan Yang ◽  
Qin-Fei Ke ◽  

La-MCS/CTS scaffolds promoted the proliferation and osteogenic differentiation of rBMSCs in vitro and bone regeneration in vivo.

2020 ◽  
Vol 70 (1) ◽  
pp. 1-15 ◽  
Barbara Dariš ◽  
Željko Knez

AbstractPoly(3-hydroxybutyrate) is a natural polymer, produced by different bacteria, with good biocompatibility and biodegradability. Cardiovascular patches, scaffolds in tissue engineering and drug carriers are some of the possible biomedical applications of poly(3-hydroxybutyrate). In the past decade, many researchers examined the different physico-chemical modifications of poly(3-hydroxybutyrate) in order to improve its properties for use in the field of bone tissue engineering. Poly(3-hydroxybutyrate) composites with hydroxyapatite and bioglass are intensively tested with animal and human osteoblasts in vitro to provide information about their biocompatibility, biodegradability and osteoinductivity. Good bone regeneration was proven when poly(3-hydroxy-butyrate) patches were implanted in vivo in bone tissue of cats, minipigs and rats. This review summarizes the recent reports of in vitro and in vivo studies of pure poly(3-hydroxy-butyrate) and poly(3-hydroxybutyrate) composites with the emphasis on their bioactivity and biocompatibility with bone cells.

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
He Zhao ◽  
Yali Ma ◽  
Dahui Sun ◽  
Wendi Ma ◽  
Jihang Yao ◽  

DEX and rhBMP2-loaded core-shell nanofiber membranes were synthesized by electrospinning method in one step. Zein/PLLA, Zein-DEX/PLLA, Zein/PLLA-rhBMP2, and Zein-DEX/PLLA-rhBMP2 were fabricated; and morphology, hydrophilicity, mechanics properties, in vitro drug release behavior, cell proliferation, and osteogenic differentiation were investigated. The results showed that the dual-release system containing rhBMP2 and DEX prepared by electrospinning had rough surface, constant drug release behavior, and could also significantly promote cell proliferation and osteogenic differentiation of RMSCs, indicating that the scaffolds we fabricated might be suitable for bone tissue engineering.

2020 ◽  
Vol 21 (21) ◽  
pp. 8352 ◽  
Teresa Marques-Almeida ◽  
Vanessa F. Cardoso ◽  
Miguel Gama ◽  
Senentxu Lanceros-Mendez ◽  
Clarisse Ribeiro

The morphological clues of scaffolds can determine cell behavior and, therefore, the patterning of electroactive polymers can be a suitable strategy for bone tissue engineering. In this way, this work reports on the influence of poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) electroactive micropatterned scaffolds on the proliferation and differentiation of bone cells. For that, micropatterned P(VDF-TrFE) scaffolds were produced by lithography in the form of arrays of lines and hexagons and then tested for cell proliferation and differentiation of pre-osteoblast cell line. Results show that more anisotropic surface microstructures promote bone differentiation without the need of further biochemical stimulation. Thus, the combination of specific patterns with the inherent electroactivity of materials provides a promising platform for bone regeneration.

2010 ◽  
Vol 93-94 ◽  
pp. 121-124
Nuttapon Vachiraroj ◽  
Siriporn Damrongsakkul ◽  
Sorada Kanokpanont

In this work, we developed a 3-dimensional bone tissue engineering scaffold from type B gelatin and hydroxyapatite. Two types of scaffolds, pure gelatin (pI~5) (Gel) and gelatin/hydroxyapatite (30/70 wt./wt.) (Gel/HA), were prepared from concentrated solutions (5% wt./wt.) using foaming/freeze drying method. The results SEM revealed the interconnected-homogeneous pores of Gel and Gel/HA were 121  119 and 148  83m, respectively. Hydroxyapatite improved mechanical property of the gelatin scaffolds, especially at dry state. Compressive modulus of Gel and Gel/HA scaffolds were at 118±21.68 and 510±109.08 kPa, respectively. The results on in vitro cells culture showed that Gel/HA scaffolds promoted attachment of rat’s mesenchymal stem cells (MSC) to a 1.23 folds higher than the Gel scaffolds. Population doubling time (PDT) of MSC on Gel and Gel/HA scaffolds were 51.16 and 54.89 hours, respectively. In term of osteogenic differentiation, Gel/HA scaffolds tended to enhance ALP activity and calcium content of MSC better than those of the Gel scaffold. Therefore the Gel/HA scaffolds had a potential to be applied in bone tissue engineering.

2018 ◽  
pp. 461-475 ◽  
Ozan Karaman

The limitation of orthopedic fractures and large bone defects treatments has brought the focus on fabricating bone grafts that could enhance ostegenesis and vascularization in-vitro. Developing biomimetic materials such as mineralized nanofibers that can provide three-dimensional templates of the natural bone extracellular-matrix is one of the most promising alternative for bone regeneration. Understanding the interactions between the structure of the scaffolds and cells and therefore the control cellular pathways are critical for developing functional bone grafts. In order to enhance bone regeneration, the engineered scaffold needs to mimic the characteristics of composite bone ECM. This chapter reviews the fabrication of and fabrication techniques for fabricating biomimetic bone tissue engineering scaffolds. In addition, the chapter covers design criteria for developing the scaffolds and examples of enhanced osteogenic differentiation outcomes by fabricating biomimetic scaffolds.

2018 ◽  
Vol 6 (5) ◽  
pp. 1147-1158 ◽  
Xiaowei Wu ◽  
Shang Zheng ◽  
Yuanzhou Ye ◽  
Yuchen Wu ◽  
Kaili Lin ◽  

The reconstruction of bone defects by guiding autologous bone tissue regeneration with graphene-based biomaterials is a potential strategy in the area of bone tissue engineering.

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Xiongfeng Tang ◽  
Yanguo Qin ◽  
Xinyu Xu ◽  
Deming Guo ◽  
Wenli Ye ◽  

For bone tissue engineering, the porous scaffold should provide a biocompatible environment for cell adhesion, proliferation, and differentiation and match the mechanical properties of native bone tissue. In this work, we fabricated porous polyetherimide (PEI) scaffolds using a three-dimensional (3D) printing system, and the pore size was set as 800 μm. The morphology of 3D PEI scaffolds was characterized by the scanning electron microscope. To investigate the mechanical properties of the 3D PEI scaffold, the compressive mechanical test was performed via an electronic universal testing system. For the in vitro cell experiment, bone marrow stromal cells (BMSCs) were cultured on the surface of the 3D PEI scaffold and PEI slice, and cytotoxicity, cell adhesion, and cell proliferation were detected to verify their biocompatibility. Besides, the alkaline phosphatase staining and Alizarin Red staining were performed on the BMSCs of different samples to evaluate the osteogenic differentiation. Through these studies, we found that the 3D PEI scaffold showed an interconnected porous structure, which was consistent with the design. The elastic modulus of the 3D PEI scaffold (941.33 ± 65.26 MPa) falls in the range of modulus for the native cancellous bone. Moreover, the cell proliferation and morphology on the 3D PEI scaffold were better than those on the PEI slice, which revealed that the porous scaffold has good biocompatibility and that no toxic substances were produced during the progress of high-temperature 3D printing. The osteogenic differentiation level of the 3D PEI scaffold and PEI slice was equal and ordinary. All of these results suggest the 3D printed PEI scaffold would be a potential strategy for bone tissue engineering.

2020 ◽  
Vol 10 (9) ◽  
pp. 1524-1530
Jing-Bo Xu ◽  
Fei Peng ◽  
Youlu Che ◽  
Wei Zhang ◽  
Changyun Quan

Biomimetic peptide has attracted extensive attention in bone tissue repairing owing to its excellent biocompatibility and stability. Hydroxyapatite ceramics (HAP) possess both excellent mechanical properties and good biocompatibility. To study the effects of bionic peptide D9KIPKAS(pSer)VPTELSAISRGDS on the interfacial activity and biological properties of hydroxyapatite ceramics, porous HAP ceramics were prepared using ammonium carbonate as a pore-forming agent. To explore the influence of surface modification on the interfacial activity of porous HAP ceramics when applying different methods, surface modification was carried out using physical adsorption (HAP-p-PP2) and a chemically grafted polypeptide (HAP-c-PP2). X-ray diffraction was used to characterize the crystal morphology of the porous HAP ceramics before and after sintering. The results of FTIR and XPS showed that bionic peptides were successfully grafted onto the surface of a porous HAP ceramic. An SEM graph shows the adhesion and spread of BMSCs on the materials. Meanwhile, the results of in vitro cell experiments showed that HAP-c-PP2 can better promote BMSC proliferation. In conclusion, bionic peptide D9KIPKAS(pSer)VPTELSAISRGDS with multifunctional functional groups is more conducive to the adhesion, proliferation and differentiation of BMSCs which can make it play an effective role in osteoinduction in bone tissue engineering.

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