Improved detection of Little cherry virus-2 using a hydrolysis probe to manage the Pacific Northwest little cherry disease epidemic.

Plant Disease ◽  
2022 ◽  
Author(s):  
Madalyn Shires ◽  
Alice Wright ◽  
Scott Harper
Keyword(s):  
Pacific Northwest ◽  
Quantitative Polymerase Chain Reaction ◽  
Leaf Tissue ◽  
Washington State ◽  
Viral Pathogen ◽  
Disease Occurrence ◽  
The Pacific ◽  
Field Samples ◽  
Potential Inhibitors ◽  
Root Tissues

Little cherry virus-2 (LChV-2) is a viral pathogen that is reaching epidemic levels in Washington state. This virus is insect-vectored and has significant impacts on sweet cherry production. To aid growers in making informed management decisions we sought to develop a diagnostic assay to better detect isolates of LChV-2 currently found in Washington, allowing for more accurate estimations of disease occurrence. This study showed that there were two distinct genotypes of LChV-2 present in Washington state. This information was used to develop an up-to-date reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) assay, which was then optimized, validated, and compared to four previously published assays using a panel of field samples. This comparison demonstrated that the newly developed assay provided greater sensitivity, accurately detecting less than 10 copies per reaction and could detect both LChV-2 genotypes. Finally, we examined the effect of potential inhibitors in various tissue types from cherry, finding that young leaf tissue affected sensitivity of detection less than root tissues.

scholarly journals Identification of a Kulshan caldera correlative tephra in the Palouse loess of Washington State, northwest USA

2016 ◽  
Vol 86 (2) ◽  
pp. 232-241 ◽  
Author(s):  
Georgina E. King ◽  
Nicholas J.G. Pearce ◽  
Helen M. Roberts ◽  
Victoria C. Smith ◽  
John A. Westgate ◽  
...  
Keyword(s):  
Trace Element ◽  
Pacific Northwest ◽  
Washington State ◽  
Fractional Crystallisation ◽  
Early Pleistocene ◽  
East Central ◽  
Trace Element Chemistry ◽  
The Pacific ◽  
Eruptive Volume ◽  
Reversed Polarity

AbstractThe Kulshan caldera formed at ∼1.15 Ma on the present-day site of Mt. Baker, Washington State, northwest USA and erupted a compositionally zoned (dacite-rhyolite) magma and a correlative eruptive, the Lake Tapps tephra. This tephra has previously been described, but only from the Puget Lowland of NW Washington. Here an occurrence of a Kulshan caldera correlative tephra is described from the Quaternary Palouse loess at the Washtucna site (WA-3). Site WA-3 is located in east-central Washington, ∼340 km southeast of the Kulshan caldera and ∼300 km east-southeast of the Lake Tapps occurrence in the Puget Lowland. Major- and trace element chemistry and location of the deposit at Washtucna within reversed polarity sediments indicates that it is not correlative with the Mesa Falls, Rockland, Bishop Ash, Lava Creek B or Huckleberry Ridge tephras. Instead the Washtucna deposit is related to the Lake Tapps tephra by fractional crystallisation, but is chemically distinct, a consequence of its eruption from a compositionally zoned magma chamber. The correlation of the Washtucna occurrence to the Kulshan caldera-forming eruption indicates that it had an eruptive volume exceeding 100 km3, and that its tephra could provide a valuable early-Pleistocene chronostratigraphic marker in the Pacific Northwest.

scholarly journals Effects of projected climate change on energy supply and demand in the Pacific Northwest and Washington State

2010 ◽  
Vol 102 (1-2) ◽  
pp. 103-128 ◽  
Author(s):  
Alan F. Hamlet ◽  
Se-Yeun Lee ◽  
Kristian E. B. Mickelson ◽  
Marketa M. Elsner
Keyword(s):  
Climate Change ◽  
Pacific Northwest ◽  
Energy Supply ◽  
Supply And Demand ◽  
Washington State ◽  
The Pacific

First Report of Downy Mildew of Chives Caused by Peronospora destructor in the Pacific Northwest

2003 ◽  
Vol 4 (1) ◽  
pp. 38
Author(s):  
Dean A. Glawe
Keyword(s):  
Downy Mildew ◽  
Pacific Northwest ◽  
Puget Sound ◽  
Washington State ◽  
King County ◽  
Specialty Crops ◽  
First Report ◽  
Peronospora Destructor ◽  
The Pacific ◽  
Allium Schoenoprasum

Chive (Allium schoenoprasum L.) is one of the specialty crops grown by farmers in the Puget Sound region of Washington State. In September, 2002, downy mildew symptoms were observed in a 0.2 hectare field planting of chive near Fall City, King County, WA. Downy mildew had not been reported previously on chive in the Pacific Northwest. Accepted for publication 15 April 2003. Published 12 May 2003.

First Report of Powdery Mildew of Myosotis sylvatica (Wood Forget-me-not) Caused by Golovinomyces cynoglossi (Erysiphe cynoglossi) in Washington State

2004 ◽  
Vol 5 (1) ◽  
pp. 18
Author(s):  
Dean A. Glawe
Keyword(s):  
Powdery Mildew ◽  
Pacific Northwest ◽  
Washington State ◽  
Causal Organism ◽  
First Report ◽  
The Pacific ◽  
Western Washington ◽  
Symptoms And Signs

Wood forget-me-not cultivars are popular ornamentals in the Pacific Northwest. In western Washington, this species frequently displays symptoms and signs of powdery mildew after anthesis. This report records the disease from King, Pierce, Snohomish, and Skagit counties and describes and illustrates symptoms of the disease as well as taxonomically important features of the causal organism. Accepted for publication 29 October 2004. Published 24 November 2004.

A Personal Journey toward Teaching Success

1994 ◽  
Vol 44 (2) ◽  
pp. 69-72
Author(s):  
Margaret Pressley ◽  
Rebecca Henry
Keyword(s):  
Music Education ◽  
Pacific Northwest ◽  
Music Teachers ◽  
Washington State ◽  
Indiana University ◽  
Violin Pedagogy ◽  
Music Festival ◽  
The Pacific ◽  
The University ◽  
Washington University

Margaret Pressley is well known in the Pacific Northwest as a teacher of gifted pre-college violinists and as an enabler of conservatory-level music education in Seattle. Attending the University of Washington, with a major in violin performance, she chose a career in violin pedagogy, which has spanned 30 years. Pressley has built a highly successful class of continuously prize-winning students, who are eagerly sought by conservatories. She is the founder and director of the Pressley Conservatory of Music in Seattle. Pressley is a lecturer at Western Washington University and is also on the faculty of the Indiana University Summer String Academy, a member of the advisory board of the Seattle Young Artist Music Festival and the National Music Teachers Association Competition String Repertoire Committee, and string chair for the Washington State Music Teachers Association. She was named ASTA's 1994 Washington State Studio Teacher of the Year.

Post-earthquake Prioritization of Bridge Inspections

2007 ◽  
Vol 23 (1) ◽  
pp. 131-146 ◽  
Author(s):  
R. T. Ranf ◽  
M. O. Eberhard ◽  
S. Malone
Keyword(s):  
Ground Motion ◽  
Pacific Northwest ◽  
Epicentral Distance ◽  
Fragility Curves ◽  
Washington State ◽  
Motion Processing ◽  
The Pacific ◽  
Bridge Damage ◽  
Washington State Department ◽  
Prioritization Strategy

Bridge damage reports from the 2001 Nisqually earthquake were correlated with estimates of ground-motion intensity at each bridge site (obtained from ShakeMaps) and with bridge properties listed in the Washington State Bridge Inventory. Of the ground-motion parameters considered, the percentage of bridges damaged correlated best with the spectral acceleration at a period of 0.3 s. Bridges constructed before the 1940s, movable bridges, and older trusses were particularly vulnerable. These bridge types were underestimated by the HAZUS procedure, which categorizes movable bridges and older trusses as “other” bridges. An inspection prioritization strategy was developed that combines ShakeMaps, the bridge inventory and newly developed fragility curves. For the Nisqually earthquake, this prioritization strategy would have made it possible to identify 80% of the moderately damaged bridges by inspecting only 481 (14%) of the 3,407 bridges within the boundaries of the ShakeMap. To identify these bridges using a prioritization strategy based solely on epicentral distance, it would have been necessary to inspect 1,447 (42%) bridges. To help the Washington State Department of Transportation (WSDOT) rapidly identify damaged bridges, the prioritization procedure has been incorporated within the Pacific Northwest Seismic Network (PNSN) ground-motion processing and notification software.

THE SPECIES OF THE NORTH AMERICAN LEAFHOPPERSCERATAGALLIAKIRKALDY ANDACERATAGALLIAKIRKALDY (RHYNCHOTA: HOMOPTERA: CICADELLIDAE)

1998 ◽  
Vol 130 (4) ◽  
pp. 427-490 ◽  
Author(s):  
K.G.A. Hamilton
Keyword(s):  
New Species ◽  
Pacific Northwest ◽  
North American ◽  
Rocky Mountains ◽  
Pacific Coast ◽  
Washington State ◽  
New Combinations ◽  
The North ◽  
The Pacific ◽  
Two New Species

AbstractThe North American genusCeratagalliaKirkaldy, 1907 is redefined to include subgenusAceratagalliaKirkaldy, 1907 (=IoniaBall, 1933, syn.nov.) with 78 species in two subgenera. Two additional new species are unplaced to subgenus:C. aceratafrom Oregon, andC. emarginatafrom Mexico. The typical subgenusCeratagalliahas 30 species, includingC. gillettei(Osborn & Ball, comb.nov.),C. sordida(Oman, comb.nov.), and two new speciesC. anafrom Mexico andC. viperafrom Washington state. SubgenusAceratagalliahas 46 species, all new combinations underCeratagallia. The economic "species" formerly known as "sanguinolenta" is divided into the Canadian clover leafhopperC. humilis(Oman) and the American clover leafhopperC. agricolasp.nov. Other new taxa in subgenusAceratagalliainclude 18 new species and seven new subspecies:alaskana(ssp. ofsiccifolia)from Alaska;omanion the Pacific coast from Oregon to British Columbia;clinoandlophiafrom the Oregon interior;compressa(ssp. ofsiccifolia),gallus,modesta,okanagana, andzacki(ssp. ofnanella) from intermontane valleys of the Pacific northwest and southwestern mountains;interior(ssp. ofhumilis) androssifrom the Sonoran subregion;australis(ssp. ofnanella),coma,ebena,entoma,falcata,oionus, andvenosafrom Mexico and Texas;alvarana(ssp. ofhumilis),cerea,cristula,harrisi, semiarida, andviatorwidespread between the Appalachian and Rocky Mountains; andwhitcombi(ssp. ofrobusta) from Florida to Arizona. Four former species are reduced to subspecies:compactaOman andpoudrisOman inC. robusta(Oman),helveolaOman inC. cinerea(Osborn & Ball), andtruncataOman inC. humilis. The taxa are keyed and illustrated, and their phylogeny is discussed.

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