DETECTION OF HLA IgG ANTIBODIES BY TWO ENZYME-LINKED IMMUNOASSAYS, SOLUBILIZED HLA CLASS I AND PRA-STAT

1995 ◽  
Vol 60 (12) ◽  
pp. 1588-1594 ◽  
Author(s):  
Christopher F. Bryan ◽  
Karen A. Baier ◽  
Gloria Flora-Ginter ◽  
Charles F. Shield ◽  
Bradley A. Warady ◽  
...  
Keyword(s):  
Class I ◽  
1995 ◽  
pp. 213-220
Author(s):  
I. Mercier ◽  
L. Glanville ◽  
L. Ellingson ◽  
L. Igoudin ◽  
N. Vanpouille ◽  
...  
Keyword(s):  
Class I ◽  

2005 ◽  
Vol 12 (4) ◽  
pp. 243-248 ◽  
Author(s):  
Hélène Odent-Malaure ◽  
Fabienne Quainon ◽  
Pascale Ruyer-Dumontier ◽  
Soizick Ducroz ◽  
Philippe Verdier ◽  
...  

TRALI is considered a serious hazard among immune complications of blood transfusion and its occurrence is admitted to be globally underestimated. Each type of blood product is likely to cause TRALI. We report here on two consecutive observations of TRALI caused by red blood cell concentrates, in which anti-HLA class I and class II antibodies resulting from post-gravitational allo-immunization were evidenced in donors. HLA class I and II antigenic community between recipients and donors' husbands were found and strong reacting IgG antibodies directed at several of those common antigens were detected in the donors' serum. Both donors had more than 3 pregnancies, raising the issue of blood donor selection or of plasma reduction for cellular products.


Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 39-39
Author(s):  
Robert S. Nickel ◽  
Jeanne E. Hendrickson ◽  
Leslie S. Kean ◽  
Marianne E. McPherson Yee ◽  
Robert A. Bray ◽  
...  

Abstract Introduction Patients with sickle cell disease (SCD) are at high risk for graft rejection after hematopoietic stem cell transplant (HSCT) especially when using reduced intensity conditioning (RIC). Alloimmunization from red blood cell (RBC) transfusions, critical to the management of SCD, may predispose these patients to HSCT rejection. Alloimmunization to major or minor histocompatibility antigens (mHA) may occur after RBC transfusion due to residual white blood cells or platelets present in RBC units even after leukoreduction. In mice, despite extensive leukoreduction, RBC transfusions caused alloimmunization and subsequent HSCT rejection after RIC. We conducted a cross-sectional study to determine if RBC transfusions are associated with alloantibody formation to human leukocyte antigens (HLA) and mHA, the target of the host versus graft rejection in HLA-identical transplantation. For mHA, we assessed immunity to H-Y antigens, mHA encoded on the Y chromosome. We have previously demonstrated the importance of H-Y antibodies in a variety of settings including chronic graft versus host disease (in male recipients of female grafts) and rejection of renal transplants (in female recipients of male grafts). Methods We enrolled 114 nulliparous pediatric SCD (SS or Sβ0) patients: 58 females on chronic transfusion, 32 males on chronic transfusion, and 24 females who had never been transfused. Serum was evaluated for HLA antibodies using the FlowPRA® (panel reactive antibody) screening test which detects IgG antibodies to the majority of HLA class I and II antigens. Serum was also tested for IgG antibodies against 5 H-Y antigens (DDX3Y [DBY], EIF1AY, RPS4Y1, UTY, ZFY) using protein microarray technology with each H-Y-seropositive threshold determined as a microarray mean fluorescence intensity (MFI) > the median MFI + 2.5 quartiles measured in 60 adult healthy males. Results Chronically transfused patients had a higher prevalence of HLA class I antibodies than never transfused patients (33.3% vs. 12.5%, p=0.074). This increased prevalence of HLA class I antibodies in the chronic transfusion group was significant when examining the number of patients who tested positive to more than 25% of the panel (17.8% vs. 0%, p=0.022). Few patients had detectable HLA class II antibodies with no significant differences between chronically transfused and non-transfused patients. Few SCD patients had detectable H-Y alloantibodies with no significant differences in chronically transfused females, chronically transfused males, and non-transfused females. Conclusions Leukocyte-reduced RBC transfusions appear to engender immunization to class I HLA but not class II HLA or H-Y antigens in pediatric SCD patients. Since hematopoietic progenitor cells express class I HLA, transfusion-related immunity to these antigens could pose a barrier to engraftment in HLA-disparate transplants. Since these antigens are also expressed on platelets, class I HLA antibodies in SCD could have important implications for platelet transfusion support during HSCT. While RBC transfusion does not appear to be an adequate stimulus for H-Y alloantibody formation, it is possible that exposure to mHA during transfusion could cause a cellular rather than a humoral immune response that may contribute to future HSCT graft rejection. Our result that few (6%) nulliparous female children with SCD had detectable H-Y alloantibodies is also a significant finding considering that 19-41% of healthy adult females had antibodies to at least one H-Y antigen in previous studies. Finally our finding that 25% of never transfused children with SCD had a positive HLA PRA is unexpected for an “unsensitized” group; its significance is being further investigated. Disclosures: No relevant conflicts of interest to declare.


1994 ◽  
Vol 39 (2) ◽  
pp. 142
Author(s):  
I. Mercier ◽  
L. Glanville ◽  
L. Igoudin ◽  
N. Vanpouille ◽  
P. Pouletty ◽  
...  
Keyword(s):  
Class I ◽  

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