scholarly journals Modified use of real-time PCR detection of group B Streptococcus in pregnancy

2017 ◽  
Vol 66 (10) ◽  
pp. 1516-1520 ◽  
Author(s):  
Ali A. Rabaan ◽  
Justin V. Saunar ◽  
Ali M. Bazzi ◽  
Joan L. Soriano
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Group B Streptococcus ◽  
Pcr Detection ◽  
Group B ◽  
In Pregnancy

scholarly journals Evaluation of StrepBSelectChromogenic Medium and the Fast-Track Diagnostics Group B Streptococcus (GBS) Real-Time PCR Assay Compared to Routine Culture for Detection of GBS during Antepartum Screening

2017 ◽  
Vol 55 (7) ◽  
pp. 2137-2142 ◽  
Author(s):  
Deirdre L. Church ◽  
Heather Baxter ◽  
Tracie Lloyd ◽  
Oscar Larios ◽  
Daniel B. Gregson
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Fast Track ◽  
Group B Streptococcus ◽  
Culture Method ◽  
Predictive Values ◽  
Content Type ◽  
Standard Culture ◽  
Group B ◽  
The Cost

ABSTRACTLife-threatening infection in neonates due to group BStreptococcus(GBS) is preventable by screening of near-term pregnant women and treatment at delivery. A total of 295 vaginal-rectal swabs were collected from women attending antepartum clinics in Calgary, Alberta, Canada. GBS colonization was detected by the standard culture method (Strep B Carrot Broth subcultured to blood agar with a neomycin disk) and compared to recovery with Strep Group B Broth (Dalynn Biologicals) subcultured to StrepBSelectchromogenic medium (CM; Bio-Rad Laboratories) and the Fast-Track Diagnostics GBS real-time PCR (quantitative PCR [qPCR]) assay (Phoenix Airmid Biomedical Corp.) performed with broth-enriched samples and the Abbottm2000sp/m2000rt system. A total of 62/295 (21%) women were colonized with GBS; 58 (19.7%) cases were detected by standard culture, while CM and qPCR each found 61 (20.7%) cases. The qPCR and CM were similar in performance, with sensitivities, specificities, and positive and negative predictive values of 98.4 and 98.4%, 99.6 and 99.6%, 98.4 and 98.4%, and 99.6 and 99.6%, respectively, compared to routine culture. Both qPCR and CM would allow more rapid reporting of routine GBS screening results than standard culture. Although the cost per test was similar for standard culture and CM, the routine use of qPCR would cost approximately four times as much as culture-based detection. Laboratories worldwide should consider implementing one of the newer methods for primary GBS testing, depending on the cost limitations of different health care jurisdictions.

scholarly journals Real-Time PCR Assay Provides Reliable Assessment of Intrapartum Carriage of Group B Streptococcus

2010 ◽  
Vol 48 (9) ◽  
pp. 3095-3099 ◽  
Author(s):  
M. J. Alfa ◽  
S. Sepehri ◽  
P. De Gagne ◽  
M. Helawa ◽  
G. Sandhu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Group B Streptococcus ◽  
Pcr Assay ◽  
Reliable Assessment ◽  
Group B

Evaluation of Culture Method and Real-Time PCR for Detection of Vaginal Group B Streptococcus Colonization in Pregnant at the Last Trimester

2014 ◽  
Vol 56 (2) ◽  
pp. 80
Author(s):  
Samer ALWEDYAN ◽  
Ramazan GMRAL ◽  
mit GOKTOLGA ◽  
Fatih SAHINER ◽  
Orhan BEDIR ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Group B Streptococcus ◽  
Culture Method ◽  
Group B
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