scholarly journals Rapid antifouling nanocomposite coating enables highly sensitive multiplexed electrochemical detection of myocardial infarction and concussion markers

2021 ◽  
Author(s):  
Sanjay Sharma Timilsina ◽  
Nolan Durr ◽  
Mohamed Yafia ◽  
Hani Sallum ◽  
Pawan Jolly ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Nanocomposite Coating ◽  
Excellent Correlation ◽  
Single Digit ◽  
Nanocomposite Layer ◽  
Sensor Platform ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
Diagnostic Applications ◽  
Detection Of Biomarkers

Here we describe an ultra-fast (< 1 min) method for coating electrochemical (EC) sensors with an anti-fouling nanocomposite layer that can be stored at room temperature for months, which provides unprecedented sensitivity and selectivity for diagnostic applications. We leveraged this method to develop a multiplexed diagnostic platform for detection of biomarkers that could potentially be used to triage patients with myocardial infarction and traumatic brain injury using only 15 microliters of blood. Single-digit pg/mL sensitivity was obtained within minutes for all the biomarkers tested in unprocessed human plasma samples and whole blood, which is much faster and at least 50 times more sensitive than traditional ELISA methods, and the signal was stable enough to be measured after one week of storage. The multiplexed EC sensor platform was validated by analyzing 22 patient samples, which demonstrated excellent correlation with reported clinical values.

Combining vancomycin-modified gold nanorod arrays and colloidal nanoparticles as the sandwich model for discrimination of gram-positive bacteria and their detection via surface-enhanced Raman spectroscopy (SERS)

The Analyst ◽  
2021 ◽  
Author(s):  
Araz Norouz Dizaji ◽  
Nihal Simsek Ozek ◽  
Ferhunde Aysin ◽  
Ayfer Calis ◽  
Asli Yilmaz ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Colloidal Nanoparticles ◽  
Nanorod Arrays ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Sensor Platform ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

This study reports the development of a highly sensitive antibiotic-based discrimination and sensor platform for the detection of gram-positive bacteria through surface-enhanced Raman spectroscopy (SERS). Herein, the combination of gold...

scholarly journals Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 85
Author(s):  
Anton Popov ◽  
Benediktas Brasiunas ◽  
Asta Kausaite-Minkstimiene ◽  
Almira Ramanaviciene
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Metal Nanoparticle ◽  
Biomarker Detection ◽  
Analytical Instruments ◽  
Electrochemical Immunosensors ◽  
Highly Sensitive ◽  
Recent Developments ◽  
Electrochemical Signal ◽  
Detection Of Biomarkers

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.

scholarly journals Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Kwabena Sarpong ◽  
Bhaskar Datta
Keyword(s):  
Nucleic Acid ◽  
High Sensitivity ◽  
Covalent Modification ◽  
Fluorescence Signal ◽  
Nucleic Acid Binding ◽  
Sensitivity And Selectivity ◽  
Target Molecules ◽  
Diagnostic Applications ◽  
The Stability ◽  
Simple Format

The binding affinity and specificity of nucleic acid aptamers have made them valuable candidates for use as sensors in diagnostic applications. In particular, chromophore-functionalized aptamers offer a relatively simple format for detection and quantification of target molecules. We describe the use of nucleic-acid-staining reagents as an effective tool for detecting and signaling aptamer-target interactions. Aptamers varying in size and structure and targeting a range of molecules have been used in conjunction with commercially available chromophores to indicate and quantify the presence of cognate targets with high sensitivity and selectivity. Our assay precludes the covalent modification of nucleic acids and relies on the differential fluorescence signal of chromophores when complexed with aptamers with or without their cognate target. We also evaluate factors that are critical for the stability of the complex between the aptamer and chromophore in presence or absence of target molecules. Our results indicate the possibility of controlling those factors to enhance the sensitivity of target detection by the aptamers used in such assays.

Application progress of magnetic molecularly imprinted polymer chemical sensors in the detection of biomarkers

The Analyst ◽  
2022 ◽  
Author(s):  
Ying Wang ◽  
Xiaomin Yang ◽  
Lin Pang ◽  
Pengfei Geng ◽  
Fang Mi ◽  
...  
Keyword(s):  
Chemical Sensors ◽  
Molecularly Imprinted Polymers ◽  
Essential Role ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Magnetic Molecularly Imprinted Polymer ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Highly Sensitive ◽  
Detection Of Biomarkers ◽  
Highly Sensitive Detection

Specific recognition and highly sensitive detection of biomarkers play an essential role in identifying, early diagnosis and prevention of many diseases. Magnetic molecularly imprinted polymers (MMIP) have been widely used...

High-sensitivity troponin assays: analytical and clinical aspects

2016 ◽  
Vol 51 (4) ◽  
pp. 315-320
Author(s):  
Magdalena Krintus
Keyword(s):  
Myocardial Infarction ◽  
Clinical Symptoms ◽  
High Sensitivity ◽  
Reference Population ◽  
Clinical Aspects ◽  
Analytical Sensitivity ◽  
Reference Limit ◽  
Highly Sensitive ◽  
Time Required ◽  
Highly Sensitive Troponin

Cardiac troponins are considered the most sensitive and specific biomarkers for the diagnosis of acute coronary syndromes (ACS). According to the Third Universal Definition of Acute Myocardial Infarction, the diagnosis requires a rise / or fall of troponin concentration with at least one value exceeding the 99th percentile upper reference limit (URL) in a reference population with the coexistence of clinical symptoms of ischemia. The introduction of highly sensitive assays has resulted in lower detection limits for the concentration of troponin, allowing for early diagnosis of, as well as the detection of quantifiable concentrations of this biomarker in healthy subjects. According to current guidelines, the use of high-sensitivity tests can shorten the time required to make clinical decisions from the current 3-6 hours to 1-2 hours. The use of highly sensitive troponin assays also carries other potential benefits associated with their predictive value, as well as challenges that include reduced specificity for myocardial infarction, lack of standardization or the presence of biological variability. Given the increasing availability of new, highly sensitive troponin assays we should be aware that their increased analytical sensitivity and precision is accompanied by accurate clinical assessment of the patient, and takes into account other non-cardiac causes of their increased concentrations.

A highly sensitive turn-on ratiometric luminescent probe based on postsynthetic modification of Tb3+@Cu-MOF for H2S detection

2017 ◽  
Vol 5 (38) ◽  
pp. 9943-9951 ◽  
Author(s):  
Xubin Zheng ◽  
Ruiqing Fan ◽  
Yang Song ◽  
Ani Wang ◽  
Kai Xing ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Luminescent Probe ◽  
Turn On ◽  
Postsynthetic Modification ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
H2s Detection

A novel probe, designed and prepared through PSM of Cu-MOFs and Tb3+ions, shows high sensitivity and selectivity for H2S.

scholarly journals Cardiac Troponins: Analytical Characteristics and Diagnostic Capabilities of Modern (High-sensitive) Determination Methods

2021 ◽  
Author(s):  
Aleksey Michailovich Chaulin ◽  
Duplyakov V Dmitry
Keyword(s):  
Myocardial Infarction ◽  
Physiological State ◽  
Economic Damage ◽  
Routine Practice ◽  
Coronary Syndrome ◽  
Highly Sensitive ◽  
Sensitive Determination ◽  
Determination Methods ◽  
Cardiac Troponins

Cardiovascular diseases have a leading role in terms of morbidity, mortality, and disability of the population, causing significant socio-economic damage to all countries of the world. This circumstance requires researchers to constantly seek for new biomarkers and improve methods for determining existing biomarkers, and search for new therapeutic targets to improve diagnostic and treatment strategies. Recently, there have been some important changes in laboratory diagnostics of patients with acute coronary syndrome, due to the introduction into the routine practice of new high and ultrasensitive methods for the determination of biomarkers of injury, specific to cardiac muscle tissue, namely cardiac troponins. A key advantage of highly sensitive immunochemical assays is the ability to detect cardiac troponins in the early stages of myocardial infarction. This allows making the optimal decision on the early choice and conduct of reperfusion therapy, which significantly improves the further prognosis of patients. Among the most significant generally recognised disadvantages of highly sensitive determination methods are low specificity and a huge variety of troponin immunoassays. The decrease in specificity is reflected in the fact that cardiac troponins are no longer considered the “gold standard” of diagnosis related to Acute Myocardial Infarction (AMI) (irreversible ischaemic damage to cardiomyocytes). As a result, any damage to the myocardium, even insignificant and reversible under physiological state (physical activity, stress) and several pathological conditions, can lead to an increase in serum levels of cardiac troponins and affect the accuracy of the diagnosis. Each method for the determination of cardiac troponins, among the existing wide variety of troponin immunoassays, possesses different analytical characteristics, and detects different concentrations of troponins in the same patient. This article provides a view of current data on the biology of cardiac troponins, and defines the analytical characteristics of new high-sensitive methods for the determination of cardiac troponins.

A metal-organic framework featuring highly sensitive fluorescence sensing for Al3+ ion

CrystEngComm ◽  
2021 ◽  
Author(s):  
Yang Qiao ◽  
Zeqi Li ◽  
Mei-Hui Yu ◽  
Ze Chang ◽  
Xian-He Bu
Keyword(s):  
Metal Ions ◽  
Human Health ◽  
Metal Organic Framework ◽  
High Sensitivity ◽  
Fluorescence Sensing ◽  
Sensing Material ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
Metal Organic ◽  
Organic Framework

High sensitivity and selectivity for detection of metal ions are very important to protect human health. Fluorescent metal-organic framework (MOF) as a new sensing material has attracted more and more...

scholarly journals Development of micropillar array electrodes for highly sensitive detection of biomarkers

RSC Advances ◽  
2020 ◽  
Vol 10 (67) ◽  
pp. 41110-41119
Author(s):  
Chaozhan Chen ◽  
Bin Ran ◽  
Zhenxing Wang ◽  
Hongli Zhao ◽  
Minbo Lan ◽  
...  
Keyword(s):  
Surface Modification ◽  
Surface Area ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Detection Of Biomarkers ◽  
Highly Sensitive Detection ◽  
Micropillar Array

PDMS-based micropillar array electrodes with increased surface area and surface modification were developed to detect biomarkers with high sensitivity.

A highly sensitive and selective fluorescent turn-on chemosensor bearing a 7-diethylaminocoumarin moiety for the detection of cyanide in organic and aqueous solutions

2020 ◽  
Vol 44 (44) ◽  
pp. 19155-19165
Author(s):  
Burcu Aydıner ◽  
Ömer Şahin ◽  
Deniz Çakmaz ◽  
Gökhan Kaplan ◽  
Kerem Kaya ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Emission Intensity ◽  
High Sensitivity ◽  
Turn On ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity

Novel 7-diethylaminocoumarin based dyes showed high sensitivity and selectivity to cyanide anions in both organic and aqueous solutions, which was observed by a drastic increment in the emission intensity.

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