Capturing the start point of the virus-cell interaction with high-speed 3D single-virus tracking

The early stages of the virus-cell interaction have long evaded observation by existing microscopy methods due to the rapid diffusion of virions in the extracellular space and the large 3D cellular structures involved. Here we present an active-feedback single-virus tracking method with simultaneous volumetric imaging of the live cell environment to address this knowledge gap to present unprecedented detail to the extracellular phase of the infectious cycle. We report previously unobserved phenomena in the early stages of the virus-cell interaction, including skimming contact events at the millisecond timescale, orders of magnitude change in diffusion coefficient upon binding, and cylindrical and linear diffusion modes along filopodia. Finally, we demonstrate how this new method can move single-virus tracking from simple monolayer culture towards more tissue-like conditions by tracking single virions in tightly packed epithelial cells. This multi-resolution method presents new opportunities for capturing fast, 3D processes in biological systems.

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A 25 Gbps VCSEL driving ASIC: an attempt for ultra-high-speed front-end readout applications

Journal of Instrumentation ◽

10.1088/1748-0221/17/01/c01040 ◽

2022 ◽

Vol 17 (01) ◽

pp. C01040

Author(s):

C. Zhao ◽

D. Guo ◽

Q. Chen ◽

N. Fang ◽

Y. Gan ◽

...

Keyword(s):

High Speed ◽

Cmos Technology ◽

Test Results ◽

Eye Diagram ◽

Optical Links ◽

Optical Module ◽

Active Feedback ◽

High Bandwidth ◽

Output Driver ◽

Very High

Abstract This paper presents the design and the test results of a 25 Gbps VCSEL driving ASIC fabricated in a 55 nm CMOS technology as an attempt for the future very high-speed optical links. The VCSEL driving ASIC is composed of an input equalizer stage, a pre-driver stage and a novel output driver stage. To achieve high bandwidth, the pre-driver stage combines the inductor-shared peaking structure and the active-feedback technique. A novel output driver stage uses the pseudo differential CML driver structure and the adjustable FFE pre-emphasis technique to improve the bandwidth. This VCSEL driver has been integrated in a customized optical module with a VCSEL array. Both the electrical function and optical performance have been fully evaluated. The output optical eye diagram has passed the eye mask test at the data rate of 25 Gbps. The peak-to-peak jitter of 25 Gbps optical eye is 19.5 ps and the RMS jitter is 2.9 ps.

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High-speed volumetric imaging of neuronal activity in freely moving rodents

Nature Methods ◽

10.1038/s41592-018-0008-0 ◽

2018 ◽

Vol 15 (6) ◽

pp. 429-432 ◽

Cited By ~ 52

Author(s):

Oliver Skocek ◽

Tobias Nöbauer ◽

Lukas Weilguny ◽

Francisca Martínez Traub ◽

Chuying Naomi Xia ◽

...

Keyword(s):

Neuronal Activity ◽

High Speed ◽

Volumetric Imaging ◽

Freely Moving

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High-speed Fly-Scan Volumetric Imaging

Microscopy and Microanalysis ◽

10.1017/s1431927618002982 ◽

2018 ◽

Vol 24 (S1) ◽

pp. 498-499 ◽

Cited By ~ 1

Author(s):

Doga Gursoy

Keyword(s):

High Speed ◽

Volumetric Imaging

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A high-speed microscopic system for observation of bubble-cell interaction from a lateral direction

2017 IEEE International Ultrasonics Symposium (IUS) ◽

10.1109/ultsym.2017.8092661 ◽

2017 ◽

Author(s):

Akane Isono ◽

Nobuki Kudo

Keyword(s):

High Speed ◽

Cell Interaction ◽

Lateral Direction

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High speed UHR-OCT for in-vivo volumetric imaging of the palisades of Vogt and the cellular structure of the limbal crypts in the healthy and pathological human corneo-scleral limbus (Conference Presentation)

Ophthalmic Technologies XXVIII ◽

10.1117/12.2291291 ◽

2018 ◽

Author(s):

Kostadinka Bizheva ◽

Bingyao Tan ◽

Zohreh Hosseinaee ◽

Kirsten Carter ◽

Denise Hileeto ◽

...

Keyword(s):

Cellular Structure ◽

High Speed ◽

Volumetric Imaging

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In-vivo volumetric imaging of the cellular structure of healthy and pathological human cornea with high-speed UHR-OCT (Conference Presentation)

Ophthalmic Technologies XXVIII ◽

10.1117/12.2291216 ◽

2018 ◽

Author(s):

Zohreh Hosseinaee ◽

Bingyao Tan ◽

Kirsten Carter ◽

Denise Hileeto ◽

Luigina Sorbara ◽

...

Keyword(s):

Cellular Structure ◽

High Speed ◽

Human Cornea ◽

Volumetric Imaging

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Distal convoluted tubule sexual dimorphism revealed by advanced 3D imaging

AJP Renal Physiology ◽

10.1152/ajprenal.00441.2020 ◽

2020 ◽

Vol 319 (5) ◽

pp. F754-F764

Author(s):

Ebrahim Tahaei ◽

Richard Coleman ◽

Turgay Saritas ◽

David H. Ellison ◽

Paul A. Welling

Keyword(s):

Sexual Dimorphism ◽

High Speed ◽

Distal Convoluted Tubule ◽

Volumetric Imaging ◽

Female Mice ◽

Cellular Density ◽

The Face ◽

Antibody Labeling ◽

Male Subjects ◽

Insight Into

The thiazide-sensitive Na+-Cl− cotransporter (NCC) is more abundant in kidneys of female subjects than of male subjects. Because morphological remodeling of the distal convoluted tubule (DCT) is dependent on NCC activity, it has been generally assumed that there is a corresponding sexual dimorphism in the structure of the DCT, leading to a larger female DCT. Until now, this has never been directly examined. Here, optical clearing techniques were combined with antibody labeling of DCT segment markers, state-of-the-art high-speed volumetric imaging, and analysis tools to visualize and quantify DCT morphology in male and female mice and study the DCT remodeling response to furosemide. We found an unexpected sex difference in the structure of the DCT. Compared with the male mice, female mice had a shorter DCT, a higher cellular density of NCC, and a greater capacity to elongate in response to loop diuretics. Our study revealed a sexual dimorphism of the DCT. Female mice expressed a greater density of NCC transporters in a shorter structure to protect Na+ balance in the face of greater basal distal Na+ delivery yet have a larger reserve and structural remodeling capacity to adapt to unique physiological stresses. These observations provide insight into mechanisms that may drive sex differences in the therapeutic responses to diuretics.

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Three Dimensional Imaging of LIGA-Made Microcomponents

Journal of Manufacturing Science and Engineering ◽

10.1115/1.1812774 ◽

2004 ◽

Vol 126 (4) ◽

pp. 813-821 ◽

Cited By ~ 17

Author(s):

Douglas Chinn ◽

Peter Ostendorp ◽

Mike Haugh ◽

Russell Kershmann ◽

Thomas Kurfess ◽

...

Keyword(s):

Computer Aided Design ◽

High Speed ◽

Three Dimensional ◽

Point Clouds ◽

Three Dimensions ◽

Cad Model ◽

Volumetric Imaging ◽

Data Registration ◽

Liga Technology ◽

Coordinate Metrology

Nickel and nickel-alloy microparts sized on the order of 5–1000 microns have been imaged in three dimensions using a new microscopic technique, Digital Volumetric Imaging (DVI). The gears were fabricated using Sandia National Laboratories’ LIGA technology (lithography, molding, and electroplating). The images were taken on a microscope built by Resolution Sciences Corporation by slicing the gear into one-micron thin slices, photographing each slice, and then reconstructing the image with software. The images were matched to the original CAD (computer aided design) model, allowing LIGA designers, for the first time, to see visually how much deviation from the design is induced by the manufacturing process. Calibration was done by imaging brass ball bearings and matching them to the CAD model of a sphere. A major advantage of DVI over scanning techniques is that internal defects can be imaged to very high resolution. In order to perform the metrology operations on the microcomponents, high-speed and high-precision algorithms are developed for coordinate metrology. The algorithms are based on a least-squares approach to data registration the {X,Y,Z} point clouds generated from the component surface onto a target geometry defined in a CAD model. Both primitive geometric element analyses as well as an overall comparison of the part geometry are discussed. Initial results of the micromeasurements are presented in the paper.

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