scholarly journals High-resolution screening for marine prokaryotic and eukaryotic taxa with selective preference for PE and PET surfaces

2021 ◽  
Author(s):  
Katherine Marsay ◽  
Yuri Koucherov ◽  
Keren Davidov ◽  
Evgenia Iankelevich Kounio ◽  
Sheli Itzahri ◽  
...  

Marine plastic debris serve as substrates for the colonization of a variety of prokaryote and eukaryote organisms. Of particular interest are the microorganisms that have adapted to thrive on plastic as they may contain genes, enzymes or pathways involved in the colonization or metabolism of plastics. We implemented DNA metabarcoding with nanopore MinION sequencing to compare the one-month-old biomes of hydrolysable (polyethylene terephthalate) and non-hydrolysable (polyethylene) plastics surfaces vs. those of glass and the surrounding water in a Mediterranean Sea marina. We sequenced longer 16S rRNA, 18S rRNA and ITS barcode loci for a more comprehensive taxonomic profiling of the bacterial, protist and fungal communities respectively. Long read sequencing enabled high-resolution mapping to genera and species. Using differential abundance screening we identified 32 bacteria and five eukaryotes, that were significantly differentially abundant on PE or PET compared to glass. This approach may be used in the future to characterize the plastisphere communities and to screen for microorganisms with a plastic-metabolism potential.

MethodsX ◽  
2019 ◽  
Vol 6 ◽  
pp. 2499-2503 ◽  
Author(s):  
Judy F.C. Chow ◽  
Heidi H.Y. Cheng ◽  
Estella Y.L. Lau ◽  
William S.B. Yeung ◽  
Ernest H.Y. Ng

1982 ◽  
Vol 97 ◽  
pp. 53-54
Author(s):  
G. G. Pooley

This is a brief report on some of the work in progress at Cambridge, with emphasis on studies of extragalactic radio sources using the One-mile and 5-km telescopes; together with the 6C survey and a new instrument now being commisioned at 151 MHz, we have a frequency range of 100:1 available for high-resolution mapping. The 6C survey has resulted in the discovery of a number of giant radio galaxies, most notably NGC 6251. At 151 MHz, the beautiful jet is not prominent; we have mapped it with the One-mile and 5-km instruments and a paper (by Saunders et al.) will shortly appear in Monthly Notices. Dr Willis will speak later on the structure of this source.


2018 ◽  
Author(s):  
Paul M. Schenk ◽  
◽  
Britney E. Schmidt ◽  
Hanna G. Sizemore ◽  
Carle M. Pieters ◽  
...  

2019 ◽  
Vol 5 (7) ◽  
pp. 351-353 ◽  
Author(s):  
Kathryn Lauren Hong ◽  
Damien Redfearn ◽  
Sanoj Chacko ◽  
Jason Baley ◽  
Adrian Baranchuk ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document