scholarly journals Impact of Nosema ceranae invasion on sucrose solution consumption, midgut epithelial cell structure, and lifespan of Apis cerana cerana workers

2022 ◽  
Author(s):  
Qi Long ◽  
Minghui Sun ◽  
Xiaoxue Fan ◽  
Wende Zhang ◽  
Dingding Zhou ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Sucrose Solution ◽  
Cell Structure ◽  
Apis Cerana ◽  
Nosema Ceranae ◽  
Apis Cerana Cerana ◽  
Midgut Epithelial Cell ◽  
Asian Honeybee ◽  
Spore Load

Nosema ceranae is an intracellular fungal parasite for honeybees, leading to chronic disease named bee nosemosis with worldwide distribution. Asian honeybee (Apis cerana) is the original host for N. ceranae, but the impact of N. ceranae infection on A. cerana physiology is largely unknown. In this current work, workers of Apis cerana cerana, a subspecies of Asian honeybee, were artificially inoculated with N. ceranae spores and reared under lab conditions, followed by detection of fungal spore load as well as host sucrose solution consumption, midgut epithelial cell structure, and lifespan. The result of spore counting suggested that the spore load in the host midgut decreased significantly during 1 dpi-2 dpi, whereas that displayed an elevated trend among 2 dpi-13 dpi. The sucrose solution consumption of workers in N. ceranae-inoculated groups among 1 dpi-20 dpi was always higher than that of workers in un-inoculated groups; additionally, the difference of sucrose solution consumption between these two groups at 4 dpi, 5 dpi, and 13 dpi was of significance. Based on microscopic observation of paraffin sections, darkly stained parasites were clearly detected in the midgut epithelial cells of N. ceranae-inoculated workers at 7 dpi-10 dpi, whereas no parasite was observed in those of un-inoculated workers. In addition, the boundaries of un-inoculated host epithelial cells were intact and the darkly stained nucleus were clear, while the boundaries of midgut epithelial cells of N. ceranae-inoculated workers were blurred, the nucleus were almost disappeared, and the nucleic acid substances were diffused. Moreover, the survival rates of workers in both N. ceranae-inoculated groups and un-inoculated groups at 1 dpi-5 dpi were pretty high and then started to decrease at 5 dpi; the survival rate of workers in N. ceranae-inoculated groups was always lower than that in un-inoculated groups, with significant difference between these two groups during 11 dpi-20 dpi. These results together indicate that the quantity of fungal spores continuously elevated with the microsporidian multiplication, causing energetic stress for workers and host cell structure damage, which further negatively affected the host lifespan. Our findings offer a solid basis not only for exploring the molecular mechanism underlying N. ceranae infection but also for investigating the interaction between N. ceranae and eastern honeybee.

scholarly journals Dynamics of Nosema apis and Nosema ceranae Co-Infection Seasonally in Honey Bee (Apis mellifera L.) Colonies

2019 ◽  
Vol 63 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Asli Özkırım ◽  
Aygün Schiesser ◽  
Nevіn Keskin
Keyword(s):  
Apis Mellifera ◽  
Apis Cerana ◽  
Winter Season ◽  
Nosema Ceranae ◽  
Molecular Techniques ◽  
Winter Period ◽  
Nosema Apis ◽  
Infection Level ◽  
Seasonal Infection ◽  
Asian Honeybee

AbstractNosema apis is a pathogen spesific for the European honeybee, Apis mellifera L., while Nosema ceranae is specific for the Asian honeybee, Apis cerana. Turkey provides different environmental and host conditions for both Nosema species. The aim of the study is to determine the dynamic of N. cerenae and N. apis seasonal infection. A number of samples were collected from different apiaries between 2009-2016 years. The samples were kept at −20°C in the laboratory. Light microscopy was used for spore counting and molecular techniques were used to identify the Nosema species. The results showed that winter season had an impact on the type of Nosema as well as on infection rates. The number of N. ceranae spores decreases significantly at low temperatures (≤ 5°C). The winter period was found to be the main factor affecting nosema infection level and dominancy of Nosema ceranae. Furthermore, co-infection of both species is an indicator of the dynamics of N. apis and N. ceranae. This study suggests, that there is a dynamic prevalence among the Nosema species depending of the average winter temperature and not a replacement of N. apis by N. ceranae.

scholarly journals MicroRNA dataset of normal and Nosema ceranae-infected midguts of Apis cerana cerana workers

Data in Brief ◽  
2019 ◽  
Vol 26 ◽  
pp. 104518 ◽  
Author(s):  
Yu Du ◽  
Dingding Zhou ◽  
Huazhi Chen ◽  
Cuiling Xiong ◽  
Yanzhen Zheng ◽  
...  
Keyword(s):  
Apis Cerana ◽  
Nosema Ceranae ◽  
Apis Cerana Cerana

scholarly journals Identification and Expression Analysis of a Putative Fatty Acidbinding Protein Gene in the Asian Honeybee,Apis cerana cerana

2013 ◽  
Vol 13 (101) ◽  
pp. 1-14 ◽  
Author(s):  
Xiaoli Yu ◽  
Mingjiang Kang ◽  
Li Liu ◽  
Xingqi Guo ◽  
Baohua Xu
Keyword(s):  
Expression Analysis ◽  
Protein Gene ◽  
Apis Cerana ◽  
Apis Cerana Cerana ◽  
Asian Honeybee

scholarly journals Molecular Identification and Expressive Characterization of an Olfactory Co-Receptor Gene in the Asian Honeybee,Apis cerana cerana

2013 ◽  
Vol 13 (80) ◽  
pp. 1-14 ◽  
Author(s):  
Huiting Zhao ◽  
Pengfei Gao ◽  
Chunxiang Zhang ◽  
Weihua Ma ◽  
Yusuo Jiang
Keyword(s):  
Molecular Identification ◽  
Receptor Gene ◽  
Apis Cerana ◽  
Apis Cerana Cerana ◽  
Asian Honeybee

scholarly journals Comparative identification of microRNAs in Apis cerana cerana workers’ midguts responding to Nosema ceranae invasion

2019 ◽  
Author(s):  
Dafu Chen ◽  
Yu Du ◽  
Huazhi Chen ◽  
Haipeng Wang ◽  
Cuiling Xiong ◽  
...  
Keyword(s):  
Immune System ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Expression Profiles ◽  
Apis Cerana ◽  
Mapk Signaling ◽  
Nosema Ceranae ◽  
Apis Cerana Cerana ◽  
Small Rna Sequencing ◽  
Biological Processes

AbstractMicroRNAs (miRNAs) are endogenous small noncoding RNAs that post transcriptionally regulate gene expression and are involved in many biological processes including host-pathogen interactions. However, the potential role of miRNAs in the responses of eastern honeybees to Nosema ceranae invasion is completely unknown. Here, the expression profiles and differentially expressed miRNAs (DEmiRNAs) in the midguts of Apis cerana cerana workers 7 and 10 days post infection (dpi) with N. ceranae were investigated via small RNA sequencing and bioinformatics. In total, 529 miRNAs highly conserved between various species and 25 novel miRNAs with varied expressions were identified for the first time. In addition, stem-loop RT-PCR confirmed the expression of 16 predicted miRNAs, validating their existence. Eight up-regulated miRNAs and six down-regulated miRNAs were detected in midguts at 7 dpi, while nine and three miRNAs were significantly up-regulated and down-regulated, respectively, in midguts at 10 dpi. In addition, Venn analysis showed that five DEmiRNAs were shared, while nine and seven DEmiRNAs were specifically expressed in midguts at 7 and 10 dpi, respectively. Gene ontology analysis suggested that a portion of the DEmiRNAs and corresponding target genes were involved in various biological processes, cellular components, and molecular functions including immune system processes and response to stimulus and signaling. Moreover, KEGG pathway analysis shed light on the potential functions of some DEmiRNAs in the regulation of target genes engaged in material and energy metabolism, cellular immunity such as endocytosis and phagosome, and the humoral immune system, including the Jak-STAT and MAPK signaling pathways. Further investigation demonstrated a complex regulation network between DEmiRNAs and their target mRNAs, with miR-598-y, miR-252-y, miR-92-x and miR-3654-y at the center of the network, implying their key parts in host responses. This comprehensive miRNA transcriptome analysis demonstrated that N. ceranae invasion influenced the expression of miRNAs in the midguts of A. c. ceranae workers; the results can not only facilitate future exploration of the regulatory roles and mechanisms of miRNAs in hosts’ responses, especially their immune responses to N. ceranae, but also provide potential candidates for further investigation of the molecular mechanisms underlying eastern honeybee-microsporidian interactions.

Sequence and expression characterization of an OBP1 gene in the Asian honeybee, Apis cerana cerana (Hymenoptera: Apidae)

2013 ◽  
Vol 49 (1) ◽  
pp. 189-196 ◽  
Author(s):  
Huiting Zhao ◽  
Wenming Zhao ◽  
Pengfei Gao ◽  
Guixian Zhang ◽  
Yusuo Jiang
Keyword(s):  
Apis Cerana ◽  
Apis Cerana Cerana ◽  
Expression Characterization ◽  
Asian Honeybee

Electron Microscopic Study of the Epithelium of the Seminal Vesicle of Aging Rats

1974 ◽  
Vol 32 ◽  
pp. 138-139
Author(s):  
V. F. Allison ◽  
G. C. Fink ◽  
G. W. Cearley
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Seminal Vesicles ◽  
Epithelial Layer ◽  
Epithelial Hyperplasia ◽  
Electron Microscopic ◽  
Aging Rats ◽  
Pseudostratified Epithelium

It is well known that epithelial hyperplasia (benign hypertrophy) is common in the aging prostate of dogs and man. In contrast, little evidence is available for abnormal epithelial cell growth in seminal vesicles of aging animals. Recently, enlarged seminal vesicles were reported in senescent mice, however, that enlargement resulted from increased storage of secretion in the lumen and occurred concomitant to epithelial hypoplasia in that species.The present study is concerned with electron microscopic observations of changes occurring in the pseudostratified epithelium of the seminal vescles of aging rats. Special attention is given to certain non-epithelial cells which have entered the epithelial layer.

Intranuclear Crystals in Regenerating Canine Kidneys

1973 ◽  
Vol 31 ◽  
pp. 412-413
Author(s):  
D.G. Osborne ◽  
L.J. McCormack ◽  
M.O. Magnusson ◽  
W.S. Kiser
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Tubular Epithelial Cell ◽  
Tubular Epithelial Cells ◽  
Cell Nuclei ◽  
Crystalline Structures ◽  
Small Crystals ◽  
Membrane Bound

During a project in which regenerative changes were studied in autotransplanted canine kidneys, intranuclear crystals were seen in a small number of tubular epithelial cells. These crystalline structures were seen in the control specimens and also in regenerating specimens; the main differences being in size and number of them. The control specimens showed a few tubular epithelial cell nuclei almost completely occupied by large crystals that were not membrane bound. Subsequent follow-up biopsies of the same kidneys contained similar intranuclear crystals but of a much smaller size. Some of these nuclei contained several small crystals. The small crystals occurred at one week following transplantation and were seen even four weeks following transplantation. As time passed, the small crystals appeared to fuse to form larger crystals.

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