scholarly journals Optimization of capture protocols across species targeting up to 32000 genes and their extension to pooled DNA

2022 ◽  
Author(s):  
Cedric Mariac ◽  
Kevin Bethune ◽  
Sinara Oliveira de Aquino ◽  
Mohamed Abdelrahman ◽  
Adeline Barnaud ◽  
...  

In-solution based capture is becoming a method of choice for sequencing targeted sequence. We assessed and optimized a capture protocol in 20 different species from 6 different plant genus using kits from 20,000 to 200,000 baits targeting from 300 to 32,000 genes. We evaluated both the effectiveness of the capture protocol and the fold enrichment in targeted sequences. We proposed a protocol with multiplexing up to 96 samples in a single hybridization and showed it was an efficient and cost-effective strategy. We also extended the use of capture to pools of 100 samples and proved the efficiency of the method to assess allele frequency. Using a set of various organisms with different genome sizes, we demonstrated a correlation between the percentage of on-target reads vs. the relative size of the targeted sequences. Altogether, we proposed methods, strategies, cost-efficient protocols and statistics to better evaluate and more effectively use hybridization capture.

2021 ◽  
Vol 55 (2) ◽  
pp. 565-583
Author(s):  
Ke Zhan ◽  
Quanxiong Lu ◽  
Sengwei Xia ◽  
Congnan Guo ◽  
Sisi Zhao ◽  
...  

1995 ◽  
Vol 108 (4) ◽  
pp. A18 ◽  
Author(s):  
I Hussain ◽  
WH Farrar ◽  
EJ Sofian ◽  
TF Bader ◽  
JD Strom ◽  
...  

2021 ◽  
Author(s):  
◽  

A growing body of evidence suggests that recognition of the collective tenure rights of Indigenous Peoples, local communities, and Afro-descendants is a powerful and cost-effective strategy for addressing the climate and biodiversity crises. In spite of this, international funding for rights recognition pales in comparison to donor mobilization around alternative solutions to these crises.


Sign in / Sign up

Export Citation Format

Share Document