scholarly journals Detection of bifenthrin, bifenazate, etoxazole resistance in Tetranychus urticae collected from mint fields and hop yards using targeted sequencing and TaqMan approaches

2022 ◽  
Author(s):  
Silas Shumate ◽  
Maggie Haylett ◽  
Brenda Nelson ◽  
Nicole Young ◽  
Kurt Lamour ◽  
...  
Keyword(s):  
Integrated Pest Management ◽  
Pest Management ◽  
Pacific Northwest ◽  
Tetranychus Urticae ◽  
The United States ◽  
Targeted Sequencing ◽  
Nucleotide Polymorphisms ◽  
Agricultural Commodities ◽  
The Pacific ◽  
Taqman Qpcr

Tetranychus urticae (Koch) is an economically important pest of many agricultural commodities in the Pacific Northwest. Multiple miticides are currently registered for control including abamectin, bifenazate, bifenthrin, and extoxazole. However, populations of Tetranychus urticae have developed miticide resistance through multiple mechanisms, in many different growing regions. Producers of agricultural commodities where Tetranychus urticae infestations are problematic rely on integrated pest management tools to determine optimal control methods. Within this species multiple single nucleotide polymorphisms have been documented in different genes which are associated with miticide resistance phenotypes. The detection of these mutations through TaqMan qPCR has been suggested as a practical, quick, and reliable tool to inform agricultural producers of miticide resistance phenotypes present within their fields and have potential utility for making appropriate miticide application and integrated pest management decisions. Within this investigation we examined the use of a TaqMan qPCR-based approach to determine miticide resistance genotypes in field-collected populations of Tetranychus urticae from mint fields and hop yards in the Pacific Northwest of the United States and confirmed the results with a multiplex targeted sequencing. The results suggest the TaqMan approach accurately genotypes Tetranychus urticae populations collected from agricultural fields. The interpretation of the results, however, provide additional challenges for integrated pest management practitioners, including making miticide application recommendations where populations of Tetranychus urticae are a mix of resistant and wildtype individuals.

scholarly journals Sclerotinia Wilt of Hop (Humulus lupulus) Caused by Sclerotinia sclerotiorum in the Pacific Northwest United States

Plant Disease ◽  
2012 ◽  
Vol 96 (4) ◽  
pp. 583-583
Author(s):  
S. M. Kropf ◽  
M. L. Putnam ◽  
M. Serdani ◽  
M. C. Twomey ◽  
J. L. Woods ◽  
...  
Keyword(s):  
United States ◽  
Pacific Northwest ◽  
Sclerotinia Sclerotiorum ◽  
Pcr Amplification ◽  
Soil Surface ◽  
The United States ◽  
Morphological Characters ◽  
Nucleotide Polymorphisms ◽  
Foliar Symptoms ◽  
The Pacific

In June 2009, wilted hop bines were observed in a yard in Marion County, OR. The wilt was associated with a stem rot that occurred ~1 m from the ground near the point where bines are tied together for horticultural purposes. Samples of affected stems were submitted to the Oregon State University Plant Clinic. White hyphae and large, black sclerotia were present on the stems, with a clear delineation between healthy and diseased tissue. The pathogen was identified as Sclerotinia sclerotiorum based on morphological characters. In June 2011, bine wilting was observed on the same farm but in a different hop yard (cv. Nugget) ~10 km from the 2009 occurrence. Affected plants had upward curled leaves with necrotic margins or wilted bines that were severed at the soil line. Wilted bines tended to have smaller diameters than bines with foliar symptoms only. Of 100 plants examined, 75% displayed some foliar symptoms and 66% had at least one bine that was wilted. Yield loss was estimated at 10 to 20% due to bine wilting before cone development. Unlike the 2009 occurrence, wilted bines did not display aerial signs of S. sclerotiorum. Rather, water-soaked lesions covered in white, cottony mycelium were apparent on affected stems 2.5 to 5 cm below the soil surface, some bearing large, irregularly shaped sclerotia. Isolations made onto potato dextrose agar yielded isolates with rapid growth rates and morphological characters consistent with S. sclerotiorum (1). DNA was extracted (2) and pathogen identity was confirmed by PCR amplification and sequencing of the internal transcribed spacer regions from isolates SS001 and SS002 as described before (4). The amplicons were sequenced bidirectionally and consensus sequences were 100% similar to S. sclerotiorum (GenBank No. AAGT01000678.1). Two nucleotide polymorphisms were present that differentiated the sequences from those of 12 S. trifoliorum accessions in GenBank that could be aligned (2). Greenhouse assays utilizing a toothpick inoculation procedure (3) were conducted to fulfill Koch's postulates. Stems of five 4-week-old hop plants of cv. Agate were pierced with a toothpick colonized with S. sclerotiorum. Five control plants were similarly inoculated with toothpicks without the fungus. Inoculated plants developed symptoms similar to those observed in the field within 11 days; four of five plants inoculated with isolate SS001 and two of five plants inoculated with isolate SS002 completely wilted. S. sclerotiorum was reisolated from all inoculated plants but not the control plants. To our knowledge, this is the first report of Sclerotinia wilt on hop in Oregon or the Pacific Northwest (1), where nearly all commercial hop production occurs in the United States. The disease appears to be localized to a limited number of yards, although given the widespread distribution and host range of S. sclerotiorum, it is plausible that the disease may occur in other yards. Recurrent outbreaks and spread of the disease among yards on the affected farm suggests that Sclerotinia wilt has the potential to become a perennial problem on hop and efforts to limit the introduction of S. sclerotiorum into other yards are warranted. References: (1) D. H. Gent. Page 32 in: Compendium of Hop Diseases and Pests. The American Phytopathological Society, St. Paul, MN, 2009. (2) E. N. Njambere et al. Plant Dis. 92:917, 2008. (3) M. L. Putnam. Plant Pathol. 53:252, 2004. (4) T. J. White et al. PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.

scholarly journals First record of Euxestonotus error (Hymenoptera: Platygastridae) in the Pacific Northwest, United States of America

2016 ◽  
Vol 148 (5) ◽  
pp. 616-618 ◽  
Author(s):  
E.R. Echegaray ◽  
R.N. Stougaard ◽  
B. Bohannon
Keyword(s):  
United States ◽  
New York ◽  
Pacific Northwest ◽  
United States Of America ◽  
First Record ◽  
The United States ◽  
Sitodiplosis Mosellana ◽  
Wheat Midge ◽  
The Pacific ◽  
Northwestern Montana

AbstractEuxestonotus error (Fitch) (Hymenoptera: Platygastridae) is considered part of the natural enemy complex of the wheat midge Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae). Although previously reported in the United States of America, there is no record for this species outside the state of New York since 1865. A survey conducted in the summer of 2015 revealed that E. error is present in northwestern Montana and is likely playing a role in the suppression of wheat midge populations.

scholarly journals Erysiphe trifolii Causing Powdery Mildew of Lentil (Lens culinaris)

Plant Disease ◽  
2009 ◽  
Vol 93 (8) ◽  
pp. 797-803 ◽  
Author(s):  
Renuka N. Attanayake ◽  
Dean A. Glawe ◽  
Frank M. Dugan ◽  
Weidong Chen
Keyword(s):  
Powdery Mildew ◽  
Pacific Northwest ◽  
The United States ◽  
Morphological Characters ◽  
Grain Legumes ◽  
Its Sequences ◽  
Powdery Mildew Fungus ◽  
The Pacific ◽  
Soybean Genotypes ◽  
Erysiphe Trifolii

The taxonomy of the powdery mildew fungus infecting lentil in the Pacific Northwest (PNW) of the United States was investigated on the basis of morphology and rDNA internal transcribed spacer (ITS) sequences. Anamorphic characters were in close agreement with descriptions of Erysiphe trifolii. However, teleomorphs formed chasmothecial appendages with highly branched apices, whereas E. trifolii has been described as producing flexuous or sometimes loosely branched appendages. Branched appendages have been described in Erysiphe diffusa, a fungus reported from species of Lens, Glycine, and Sophora, raising the possibility that the PNW fungus could be E. diffusa. Examination of morphological characters of an authentic specimen of E. trifolii from Austria determined that it included chasmothecial appendages resembling those seen in PNW specimens. Furthermore, ITS sequences from five powdery mildew samples collected from lentils in PNW greenhouses and fields from 2006 to 2008 were identical to one another, and exhibited higher similarity to sequences of E. trifolii (99%) than to those of any other Erysiphe spp. available in GenBank. Parsimony analysis grouped the lentil powdery mildew into a clade with Erysiphe baeumleri, E. trifolii, and E. trifolii–like Oidium sp., but indicated a more distant relationship to E. diffusa. In greenhouse inoculation studies, the lentil powdery mildew fungus did not infect soybean genotypes known to be susceptible to E. diffusa. The pathogenicity of E. trifolii on lentil was confirmed using modified Koch's postulates. This is the first report of E. trifolii infecting lentil. E. diffusa and E. trifolii have different host ranges, so the discovery of E. trifolii on lentil has implications both for determining species of powdery mildews on cool-season grain legumes, and in disease management.

Evaluation of the phytotoxicity of 2,4-diacetylphloroglucinol and Pseudomonas brassicacearum Q8r1-96 on different wheat cultivars

2021 ◽  
Author(s):  
Mingming Yang ◽  
Linda S Thomashow ◽  
David M Weller
Keyword(s):  
Seed Germination ◽  
Pacific Northwest ◽  
Root Colonization ◽  
The United States ◽  
Wheat Cultivars ◽  
The Pacific ◽  
Root Necrosis ◽  
Growth Promoting ◽  
Pseudomonas Brassicacearum

Pseudomonas brassicacearum Q8r1-96 and other 2,4-diacetylphloroglucinol (DAPG)-producing pseudomonads of the Pseudomonas fluorescens complex possess both biocontrol and growth-promoting properties and play an important role in suppression of take-all of wheat in the Pacific Northwest (PNW) of the United States. However, P. brassicacearum can also reduce seed germination and cause root necrosis on some wheat cultivars. We evaluated the effect of Q8r1-96 and DAPG on the germination of 69 wheat cultivars that have been or currently are grown in the PNW. Cultivars varied widely in their ability to tolerate P. brassicacearum or DAPG. The frequency of germination of the cultivars ranged from 0 to 0.87 and from 0.47 to 0.90 when treated with Q8r1-96 and DAPG, respectively. There was a significant positive correlation between the frequency of germination of cultivars treated with Q8r1-96 in assays conducted in vitro and in the greenhouse. The correlation was greater for spring than for winter cultivars. In contrast, the effect of Q8r1-96 on seed germination was not correlated with that of DAPG alone, suggesting that DAPG is not the only factor responsible for the phytotoxicity of Q8r1-96. Three wheat cultivars with the greatest tolerance and three cultivars with the least tolerance to Q8r1-96 were tested for their ability to support root colonization by strain Q8r1-96. Cultivars with the greatest tolerance supported significantly greater populations of strain Q8r1-96 than those with the least tolerance to the bacteria. Our results show that wheat cultivars differ widely in their interaction with P. brassicacearum and the biocontrol antibiotic DAPG.

scholarly journals If You Provide, Will They Ride? Motivators and Deterrents to Shared Micro-Mobility

2020 ◽  
pp. 26-38
Author(s):  
Ronald W. Pimentel, Ph.D. ◽  
Michael B. Lowry, Ph.D. ◽  
David Pimentel, J.D. ◽  
Amanda K. Glazer ◽  
Timothy W. Koglin ◽  
...  
Keyword(s):  
Pacific Northwest ◽  
Reasoned Action ◽  
Secondary Data ◽  
The United States ◽  
The Pacific ◽  
Micro Mobility ◽  
On Line ◽  
Optimal System Design ◽  
Depth Interviews ◽  
Bike Share

Bike share, e-bike share, and e-scooter systems (shared micro-mobility) are gaining popularity throughout the United States and internationally, but the optimal system design has not been determined. This study investigated motivators and deterrents to the use of such systems in the Pacific Northwest with secondary data, participant observations, depth interviews, and an on-line survey to users and non-users. The survey was administered in all cities in Washington, Oregon, and Idaho that have shared micro-mobility systems. The strongest motivators reported were exercise and enjoyment. The strongest deterrents were weather, danger from automobile traffic, and insufficient bike lanes and paths. The latter two deterrents might be alleviated through continued improvements to infrastructure; however, the weather cannot be changed, and neither can hills. Data were fitted to the Theory of Reasoned Action and the resulting recommendation is to promote popular motivators of exercise and enjoyment and emphasize personal benefits more than social appearances.

“How Can We Catch Fire?”

2021 ◽  
pp. 119-143
Author(s):  
Melanie C. Ross
Keyword(s):  
United States ◽  
African American ◽  
Pacific Northwest ◽  
Holy Spirit ◽  
The United States ◽  
Kingdom Of God ◽  
The Pacific ◽  
The Holy Spirit ◽  
Influence At Work ◽  
Vineyard Movement

Chapter 5 explores the Vineyard movement, one of the fastest-growing church movements in the United States, which is committed to holding together the “already” and “not yet” of the Kingdom of God in worship. In addition to looking for a dramatic, miraculous inbreaking of the Holy Spirit, there is a less dramatic but equally formative influence at work in worship: the Quaker notion of “gospel order” and its accompanying understanding of ethics. These commitments are tested at “Koinonia Vineyard,” a congregation located in the Pacific Northwest, where one African American member wrestles with her vision of activism and her Caucasian pastor’s desire for the congregation to remain politically neutral during a time of national racial unrest.

Understanding Decision Making by Families About Youth Football Participation Postconcussion

2020 ◽  
pp. 152483992094525
Author(s):  
Emily Kroshus ◽  
Deborah Bowen ◽  
Douglas Opel ◽  
Sara P. D. Chrisman ◽  
Frederick P. Rivara
Keyword(s):  
Pacific Northwest ◽  
Decision Aid ◽  
Sports Participation ◽  
The United States ◽  
Football Players ◽  
Contact Sport ◽  
Sample Mean ◽  
Youth Football ◽  
The Pacific ◽  
Northwest Region

Many families are concerned about their child’s risk of concussion, and some seek counsel from clinicians about whether or not to return to contact sports participation postinjury. The present study sought to identify factors that parents weight most heavily in forming their preferences regarding whether their child should return to contact sport after recovering from a concussion. Survey data were collected from 568 parents of youth football players (aged 7–14 years) in the Pacific Northwest region of the United States (73% response rate). Approximately two thirds (63%) of parents preferred that their child retire from football after one or two concussions. Multivariable linear regression indicated parents above the sample mean in terms of how strongly they valued football participation preferred their child stop after more concussions than parents below the sample mean (β = .44, standard error [SE] = 0.06, p < .001). Factors endorsed by the most parents as making them “much more likely” to want their child to stop playing football included the belief that their child will experience cognitive issues later in life as a result of concussions (65.0%) and that their child will get another concussion while playing football (43.5%). Within the context of a clinical visit postconcussion, physicians may need to help families clarify their values related to football participation and provide information about the potential outcomes of returning to contact sport. A formalized shared decision aid could help support consistent implementation of this potentially challenging conversation.

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