Hybridoma Screening by Antigen Capture: Immunoprecipitation
Keyword(s):
Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after sodium dodecyl sulfate (SDS)–polyacrylamide electrophoresis and thus it is simple to discriminate between true and false positives. Furthermore, the assay provides information regarding the molecular weight of the antigen.
1976 ◽
Vol 59
(12)
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pp. 2122-2125
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2003 ◽
Vol 295
(4)
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pp. 169-174
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1974 ◽
Vol 38
(10)
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pp. 1947-1953