Antioxidant potential, anti-nutritional factors, volatile compounds and phenolic composition of microwave heat-treated plum (Prunus domestica. L.) kernels: an analytical approach

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mohd Aaqib Sheikh ◽  
Charanjiv Singh Saini ◽  
Harish Kumar Sharma

PurposeThe study was aimed to explore the potential impact of microwave heating (450 W for 2, 4, 6 and 8 min) on antioxidant activity, anti-nutritional factors, volatile and phenolic compounds of the plum kernels.Design/methodology/approachPlum kernels are rich in proteins, lipids and bioactive compounds that are mostly underused and undervalued.FindingsThe results showed that microwave heating at 450 W for 6 min significantly (p < 0.05) increased the antioxidant activity, total phenolic and flavonoid content, while the longer treatment time (450 W for 8 min) adversely affected the phenolic compounds. Most importantly, the anti-nutritional factors like amygdalin, hydrocyanic acid, phytic acid and tannin content were reduced up to 87.1, 84.7, 20.9 and 46.2%, respectively at 450 W for 6 min treatment conditions, which was confirmed from the larger shifts observed in FT-IR spectra near 1,157 cm−1. Microwave heating at 450 W for 6 min also proved beneficial in improving the bioavailability of volatile and phenolic compounds including chlorogenic acid, gallic acid, syringic acid, (+)-catechin, caffeic acid, ß-carotene, trans-ferulic acid, rutin trihydrate, 3,4-dihydroxybenzoic acid, tannic acid and quercetin by liberating them from the plant matrix.Originality/valueThe results thus indicate that controlled microwave heating could be an effective approach for the reduction of anti-nutritional factors besides leading to an overall improvement in antioxidant potential and volatile and phenolic compounds. This novel technological approach can proliferate the use of plum kernels in different diversified food formulations.

2018 ◽  
Vol 48 (3) ◽  
pp. 386-396 ◽  
Author(s):  
Vania Silva Carvalho ◽  
Ana Carolina Conti-Silva

Purpose This paper aims to examine the bioactive compounds and texture properties of cereal bars formulated with banana peel flour during storage. Design/methodology/approach Seven cereal bars were produced and stored during 11 months, under vacuum and protected from the light. The total phenolic compounds, the activity antioxidant by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] method, the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) method expressed as EC50 (concentration required to reduce the original amount of free radicals by 50 per cent) and texture properties were evaluated over the storage period. Findings In general, total phenolic compounds decreased during storage (from 4.19 to 1.11 mg GAE. g-1f.w.). Although the total antioxidant activity (ABTS method) increased during the fifth month, it reduced during storage (from 3.41 to 0.30 µmol TE.g-1f.w.); and the EC50 was not modified in many formulations, though it decreased in other formulations during storage period (from 3913 to 19221 g fruit.g-1 DPPH). The force of rupture began to increase in the fourth month (reaching 62.4 N), and hardness began to increase in the ninth month (reaching 444 N). The formulation and time factors influenced the total phenolic compounds, total antioxidant activity (ABTS method), force of rupture and hardness, while EC50 was only influenced by the formulation (p-value = 0.001). A principal component analysis showed that time had little effect on the most important characteristics considered in description of the cereal bars. Originality/value Cereal bars can be consumed up to the third month of storage, considering the texture of the products. Moreover, the presence of bioactive compounds in cereal bars depends on the addition of banana peel flour, which it contributes to the insertion of total phenolic compounds and total antioxidant activity in cereal bars, aggregating functional properties in these products.


Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3082 ◽  
Author(s):  
Milena Polumackanycz ◽  
Tomasz Sledzinski ◽  
Elzbieta Goyke ◽  
Marek Wesolowski ◽  
Agnieszka Viapiana

Morus alba L. (white mulberry) has been commonly used as a functional food and for medicinal purposes. Hence, the aim of the study was to compare the phenolic profile of white mulberry commercial samples in relation to their antioxidant potential and acetylcholinesterase (AChE) inhibitory activity. It is of interest to determine whether herbal products originating from different commercial sources differ in their phenolic profiles. For this purpose, a simple and rapid high-performance liquid chromatography (HPLC) method was used for the separation and determination of ten major phenolic compounds. Total phenolic (TPC), total flavonoid (TFC), and total phenolic acid contents (TPAC), as well as l(+)-ascorbic acid (ASA) contents, were determined. The antioxidant potential was assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl radical) scavenging activity and ferric-reducing/antioxidant power (FRAP) assay, while the AChE inhibitory activity was determined by the Ellman assay for water extracts. The study revealed that excluding two herbal products containing fruits and a sample containing leaves of white mulberry, yerba mate and lemon, the remaining samples were generally consistent in terms of phenolic composition as well as antioxidant potential and AChE inhibitory activity. This reflects the health-promoting properties of the samples under study. Moreover, the results showed that the water extracts of white mulberry were richer in phenolic compounds and presented higher antioxidant activity than the hydromethanolic extracts. However, the water extracts showed low inhibitory activity against AChE. Moreover, the correlation analysis indicated a high positive relationship between phenolic composition and antioxidant activity in extracts of white mulberry. Overall, the obtained results may be useful in the evaluation of new dietary supplements and food products. The water extracts of white mulberry could be used for antioxidant purposes, while the hydromethanolic extracts could be incorporated in antioxidant formulations.


Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 243 ◽  
Author(s):  
Bimal Kumar Ghimire ◽  
Erik J. Sacks ◽  
Seung Hyun Kim ◽  
Chang Yeon Yu ◽  
Ill Min Chung

Miscanthus sacchariflorus is a potential source of sustainable biofuel and other bioactive compounds. The high adaptive range of M. sacchariflorus may cause variation in its morphological traits and phytochemical composition. Although some metabolites have been reported from M. sacchariflorus, little is known about its phenolic compound composition and antioxidant or oxidant properties. This study evaluated the morphological traits, antioxidant properties, and phenolic compound profile of M. sacchariflorus collected from various regions of China, Korea, Japan, and Russia. The antioxidant potential of the leaf extracts of various accessions of M. sacchariflorus was estimated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate (ABTS). An extensive study of the antioxidant activity and phenolic compounds of M. sacchariflorus obtained from different locations in four different countries could provide a comprehensive catalogue of the phytoconstituents and antioxidant properties of M. sacchariflorus accessions to consumers and nutraceutical industries. A total of 22 phenolic compounds were identified and quantified, among which p-coumaric acid, ferulic acid, p-hydroxybenzoic acid, chlorogenic acid, vitexin, and luteolin were the most dominant phytochemical compounds detected in the majority of the accessions. The antioxidant potential (DPPH) of the leaf extracts of all of the accessions ranged from 28.85 ± 1.53 µg mL−1 in MS-447 to 99.25 ± 1.63 µg mL−1 in MS-190. The antioxidant properties (ABTS) of the leaf extracts of all accessions ranged from 25.65 ± 2.06 in MS-258 to 83.62 ± 2.02 in MS-271. Pearson’s correlation analysis showed a significant and positive correlation between antioxidant activity and total phenolic content, and total flavonoid content varied widely among M. sacchariflorus accessions from the four geographical study regions. A strong and positive association was observed between DPPH with total phenolic content and total flavonoid content. Moderately positive correlations were observed between DPPH scavenging activity with gentisic acid, p-hydroxybenzoic acid, chlorogenic acid, p-coumaric acid, rutin, and quercetin (r = 0.385, r = 0.379, r = 0.362, r = 0.353, r = 0.490, and r = 0.372, respectively), suggesting that phenolic compounds are major contributors to the antioxidant potential of M. sacchariflorus. Thirty-two accessions collected from four different countries (China, Korea, Japan, and Russia) were characterized for 17 quantitative morphological traits. A wide range of diversity was observed in the morphological traits, with plant height ranging from 18.00 ± 1.00 cm to 163.20 ± 4.00 cm. Plant height was significantly correlated with biomass yield (fresh weight; r = 0.439, p < 0.05) and also had moderately positive correlations with culm length (r = 0.356, p < 0.05). Culm length was moderately correlated with the biomass yield fresh weight (r = 0.419*, p < 0.05) and the biomass yield dry weight (r = 0.425*, p < 0.05); however, it exhibited weak and negative correlations with compressed plant circumference (CCirc) (r = −0.374, p < 0.05) and total culm node number (TCmN) (r = −0.440, p < 0.05). Principal components analysis was performed to assess the variation in 17 morphological traits in 32 accessions of M. sacchariflorus. The first two principal components explained 51.24% of the morphological variations. A dendrogram generated from unweighted pair group method with arithmetic mean (UPGMA) clustering based on morphological characters was not found to be consistent with another dendrogram based on phytochemicals. In both cases, the number of studied accessions collected from different geographical regions grouped into two major groups. However, no clear correlation between these two different approaches was found. The substantial variation in the morphological traits, bioactive properties, and phenolic compounds among the accessions may provide useful information for breeding programs attempting to obtain M. sacchariflorus varieties with improved phenolic compounds traits and improved bioactive properties.


Author(s):  
Rodrigo Zunta Raia ◽  
Ana Paula De Souza ◽  
Beatriz Cervejeira Bolanho Barros

The effect of three different drying temperatures (60, 70, 80 ºC) was evaluated in effective diffusivity, colorimetric parameters, antioxidant activity, and betalain content of pulp (P) and mesocarp (M) of pitaya (Hylocereus costaricensis). The increase in drying temperatures had a direct influence on drying constants, showing the drying rate is faster when drying temperature of 80 ºC was used. Modified Page model described the drying behaviour of pitaya pulp and mesocarp. In the colorimetric parameters, the higher drying temperatures decreased the luminosity and the reddish tonality and increased the color saturation. The drying promoted a concentration of antioxidants compounds in relation to in natura samples. The betalain content decreased in the pulp and it was maintained in the mescocarp samples, while the total phenolic compounds and the antioxidant activity increased in both pitaya parts when higher temperatures were used in the drying process. For these reasons the temperature of 60 ºC was indicating the most appropriate temperature for temperature for pitaya pulp end mesocarp drying process.


2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Tainara Leal de Sousa ◽  
Jessyca Pinheiro da Silva ◽  
Ariadne Ribeiro Lodete ◽  
Daniele Silva Lima ◽  
Abner Alves Mesquita ◽  
...  

Purpose This study aims to analyze the levels of vitamin C, phenolic compounds and antioxidants in baby food. Providing nutritious food to the infants is essential for their growth and development. Baby foods are foods prepared from any suitable nutritive material and intended for feeding children after six months of age. The search for foods that meet the nutritional needs of children and that also promote aspects of health has increased on the part of parents. Design/methodology/approach A total of 17 different types of baby foods (2 brands) were purchased and the vitamin C content, total phenolic compounds (TPC) and antioxidant activity were determined. The authors also assessed the adequacy of labeling in accordance with regulatory parameters. Data was submitted to analysis of variance, and the means were compared by the Tukey test at 5% probability. Principal component analysis was performed to evaluate the results (phenolic compounds, vitamin C and antioxidant activity) from the multidimensional point of view. Findings In general, the labeling of baby foods proved to be within the legislation, but with confusing information for the consumer. BF7-AAPO (apple pulp and juice, papaya and orange juice), BF-BAO (banana, apple juice and oatmeal) and BF-AA (apple pulp and juice) showed the highest vitamin C content (20–28 mg/100 g; p < 0.001). BF-A (apple), BF-B (banana) and BF-AA showed the highest TPC (7–8.2 mg/100 g; p < 0.001). BF-CP (cassava-parsley), BF-PCBCP (potato, carrot, beef and cassava-parsley) and BF-PCBB (potato, carrot, beef and broccoli) showed the highest antioxidant activity (44–48%; p < 0.001). High value of TPC was related to high value of antioxidant activity in baby foods. In conclusion, vitamin C content in the baby foods was different from those reported on the labels and there was a direct relationship between the content of TPC and the antioxidant capability. Originality/value The objective of this work was to evaluate bioactive compounds and antioxidant activity in baby foods. So far, few papers have reported on the subject as the authors seek to know about essential nutrients. However, this knowledge is important because these compounds can decrease and/or prevent inflammatory processes in the body.


Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1301
Author(s):  
Eva P. Pérez-Álvarez ◽  
Diego S. Intrigliolo ◽  
María Pilar Almajano ◽  
Pilar Rubio-Bretón ◽  
Teresa Garde-Cerdán

The high phenolic compound content of grapes makes them an important source of natural antioxidants, among other beneficial health properties. Vineyard irrigation might affect berry composition and quality. Regulated deficit irrigation (RDI) is a widely used strategy to reduce the possible negative impact of irrigation on grapes, improving grape composition and resulting in water savings. Monastrell grapevines (Vitis vinifera L.) grown in eastern Spain were subjected to two water regime strategies: rainfed (non-irrigation) and RDI. The content of anthocyanins, flavonols, flavanols, hydroxybenzoic and hydroxycinnamic acids, and stilbenes was determined by HPLC and was related with total phenolic content and three antioxidant activity methods (ABTS, DPPH, and ORAC). The study aimed to evaluate and compare the phenolic composition and antioxidant potential of Monastrell grapes. The rainfed regime concentrated grapes in terms of phenolic compounds. Thus, total content of anthocyanins, flavonols, flavanols, hydroxybenzoic acids, and total phenols were higher in the rainfed grapes than in the RDI ones. Besides, the rainfed grapes doubled their antioxidant potential with respect to the RDI grapes with the ORAC method. Total phenolic content and antioxidant activity by ORAC assay positively correlated with most of the total phenolic compounds analyzed. This study demonstrates how field practices can modulate final grape composition in relation to their antioxidant activity.


2021 ◽  
Vol 42 (5) ◽  
pp. 1347-1354
Author(s):  
M.K. Berwal ◽  
◽  
S.M. Haldhar ◽  
C. Ram ◽  
P.L. Saroj ◽  
...  

Aim: The aim of the study was Identification and quantification of phenolic compounds, total flavonoides content and antioxidant potential of flower buds, foliages, bark and root of a perennial herb, Calligonum polygonoides (Phog) of Thar Desert. Methodology: The methanolic extracts of different plant parts of C. polygonoides were subjected to LC-MS/MS (Waters Acquity UPLC-PDA, TQD) analysis for phenolic identification and spectrophotometric assay of total phenolic and flavonoids content and total antioxidant activity was estimated. Results: A total of 15 phenolic compounds were identified and quantified, among which gallic acid content was abundant, followed by catechin. Besides these, the other major phenolic compounds detected in different plant parts were vanillic, chlorogenic acid, epicatechin, coumeric acid, catechol, vanillic acid, epicatechin and syringic acid. Extremely higher values were recorded for total phenolics, flavonoids and antioxidant capacity. The values for phenol, flavonoids and total antioxidant activity in bark extracts were higher than that of clove extract. Interpretation: The identified phenolic compounds possess high antioxidant potential. These results provide scientific evidence for use of C. polygonoides as safe natural antioxidant compounds in nutraceutical and pharmaceutical industry.


2020 ◽  
Vol 13 (9) ◽  
pp. 10
Author(s):  
S. M. P. C. Silva ◽  
W. C. Andrade ◽  
P. C. Santos ◽  
A. S. Nascimento ◽  
C. A. L. Carvalho

This work evaluated the bioactive compounds and the antioxidant potential of honey produced by Africanized bees (Apis mellifera L.) in the state of Piauí, Brazil. The analyses show that all honeys investigatedpresented features of the pollen type Mimosa caesalpiniifolia. The content of phenolic compounds ranged from 27.65 to 97.01 mg in GAE g-1 of honey, with an average content of 62.66 ± 20.46 mgEAG g-1 of honey. The total flavonoid contents expressed in mg of quercentin/g of honey ranged from 5.43 to 13.02 mg of QE g-1, with an average of 8.33 ± 1.86 mg of QE g-1 of honey. The best performance of antioxidant activity by reducing power was EC50 of 0.15 and the lowest performance of antioxidant activity with IC50 was 1.24. The highest antioxidant activity (DPPH) ranged from 66.11 ± 0.02mg EAQ 100g-1, while the lowest activities were 9.16 and 10.88 mg EAQ 100g-1. The results show that the contents of total phenolic compounds, total flavonoids, DPPH antioxidant activity, and the pollen-reducing power from Piauí were influenced by botanical origin. 


Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.


Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 234 ◽  
Author(s):  
Yili Hong ◽  
Zening Wang ◽  
Colin J. Barrow ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria

Stone fruits, including peach (Prunus persica L.), nectarine (Prunus nucipersica L.), plum (Prunus domestica L.) and apricot (Prunus armeniaca L.) are common commercial fruits in the market. However, a huge amount of stone fruits waste is produced throughout the food supply chain during picking, handling, processing, packaging, storage, transportation, retailing and final consumption. These stone fruits waste contain high phenolic content which are the main contributors to the antioxidant potential and associated health benefits. The antioxidant results showed that plum waste contained higher concentrations of total phenolic content (TPC) (0.94 ± 0.07 mg gallic acid equivalents (GAE)/g) and total flavonoid content (TFC) (0.34 ± 0.01 mg quercetin equivalents (QE)/g), while apricot waste contained a higher concentration of total tannin content (TTC) (0.19 ± 0.03 mg catechin equivalents (CE)/g) and DPPH activity (1.47 ± 0.12 mg ascorbic acid equivalents (AAE)/g). However, nectarine waste had higher antioxidant capacity in ferric reducing-antioxidant power (FRAP) (0.98 ± 0.02 mg AAE/g) and total antioxidant capacity (TAC) (0.91 ± 0.09 mg AAE/g) assays, while peach waste showed higher antioxidant capacity in 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay (0.43 ± 0.09 mg AAE/g) as compared to other stone fruits waste. Qualitative and quantitative phenolic analysis of Australian grown stone fruits waste were conducted by liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) and HPLC-photodiode array detection (PDA). The LC-ESI-QTOF-MS/MS result indicates that 59 phenolic compounds were tentatively characterized in peach (33 compounds), nectarine (28), plum (38) and apricot (23). The HPLC-PDA indicated that p-hydroxybenzoic acid (18.64 ± 1.30 mg/g) was detected to be the most dominant phenolic acid and quercetin (19.68 ± 1.38 mg/g) was the most significant flavonoid in stone fruits waste. Hence, it could be concluded that stone fruit waste contains various phenolic compounds and have antioxidant potential. The results could support the applications of these stone fruit wastes in other food, feed, nutraceutical and pharmaceutical industries.


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