Bioinformatics analysis of miRNAs identifies enrichment of axon guidance pathway genes in ovarian cancer stem cells

Author(s):  
Shurui Cai ◽  
Renata Fu ◽  
Kevin Wang ◽  
Na Li ◽  
Haowen Chen ◽  
...  
2020 ◽  
Vol 10 ◽  
Author(s):  
Nastassja Terraneo ◽  
Francis Jacob ◽  
Anna Dubrovska ◽  
Jürgen Grünberg

2019 ◽  
Vol 39 (3) ◽  
Author(s):  
Wenxiang Wang ◽  
Yuxia Gao ◽  
Jing Hai ◽  
Jing Yang ◽  
Shufeng Duan

Abstract Increasing evidence shows that cancer stem cells are responsible for drug resistance and relapse of tumors. In breast cancer, human epidermal growth factor receptor 2 (HER2) induces Herceptin resistance by inducing cancer stem cells. In the present study, we explored the effect of HER2 on cancer stem cells induction and drug sensitivity of ovarian cancer cell lines. First, we found that HER2 overexpression (HER2 OE) induced, while HER2 knockdown (HER2 KD) decreased CD44+/CD24− population. Consistently, HER2 expression was closely correlated with the sphere formation efficiency (SFE) of ovarian cancer cells. Second, we found that NFκB inhibition by specific inhibitor JSH23 or siRNA targetting subunit p65 dramatically impaired the induction of ovarian cancer stem cells by HER2, indicating that NFκB mediated HER2-induced ovarian cancer stem cells. Third, we found that HER2 KD significantly attenuated the tumorigenicity of ovarian cancer cells. Further, we found that HER2 inhibition increased drastically the sensitivity of ovarian cancer cells to doxorubicin (DOX) or paclitaxel (PTX). Finally, we examined the correlation between HER2 status and stem cell-related genes expression in human ovarian tumor tissues, and found that expressions of OCT4, COX2, and Nanog were higher in HER2 positive tumors than in HER2 negative tumors. Consistently, the 5-year tumor-free survival rate of HER2 positive patients was dramatically lower than HER2 negative patients. Taken together, our data indicate that HER2 decreases drug sensitivity of ovarian cancer cells via inducing stem cell-like property.


Author(s):  
Tao Yang ◽  
Jianan Cheng ◽  
Junhao You ◽  
Bing Yan ◽  
Hui Liu ◽  
...  

Stem Cells ◽  
2016 ◽  
Vol 34 (3) ◽  
pp. 551-564 ◽  
Author(s):  
Eun Jin Seo ◽  
Yang Woo Kwon ◽  
Il Ho Jang ◽  
Dae Kyoung Kim ◽  
Soo In Lee ◽  
...  

2019 ◽  
Vol 55 (43) ◽  
pp. 6106-6109 ◽  
Author(s):  
Morgan Stilgenbauer ◽  
Amarasooriya M. D. S. Jayawardhana ◽  
Payel Datta ◽  
Zhizhou Yue ◽  
Michael Gray ◽  
...  

A spermine-conjugated lipophilic Pt(iv) prodrug is designed to induce mitochondrial damage and eliminate ovarian cancer stem cells.


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