scholarly journals Fast Acoustic Wave Sparsely Activated Localization Microscopy: Ultrasound Super-Resolution Using Plane-Wave Activation of Nanodroplets

2019 ◽  
Vol 66 (6) ◽  
pp. 1039-1046 ◽  
Author(s):  
Ge Zhang ◽  
Sevan Harput ◽  
Hanyu Hu ◽  
Kirsten Christensen-Jeffries ◽  
Jiaqi Zhu ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Plane Wave ◽  
Super Resolution ◽  
Localization Microscopy

scholarly journals Acoustic wave sparsely activated localization microscopy (AWSALM): Super-resolution ultrasound imaging using acoustic activation and deactivation of nanodroplets

2018 ◽  
Vol 113 (1) ◽  
pp. 014101 ◽  
Author(s):  
Ge Zhang ◽  
Sevan Harput ◽  
Shengtao Lin ◽  
Kirsten Christensen-Jeffries ◽  
Chee Hau Leow ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Ultrasound Imaging ◽  
Super Resolution ◽  
Localization Microscopy

scholarly journals Comparing Super-Resolution Microscopy Techniques to Analyze Chromosomes

2021 ◽  
Vol 22 (4) ◽  
pp. 1903
Author(s):  
Ivona Kubalová ◽  
Alžběta Němečková ◽  
Klaus Weisshart ◽  
Eva Hřibová ◽  
Veit Schubert
Keyword(s):  
Single Molecule ◽  
Imaging Techniques ◽  
Chromatin Condensation ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Wide Field ◽  
Structured Illumination Microscopy ◽  
Metaphase Chromosomes ◽  
Localization Microscopy ◽  
Super Resolution Microscopy

The importance of fluorescence light microscopy for understanding cellular and sub-cellular structures and functions is undeniable. However, the resolution is limited by light diffraction (~200–250 nm laterally, ~500–700 nm axially). Meanwhile, super-resolution microscopy, such as structured illumination microscopy (SIM), is being applied more and more to overcome this restriction. Instead, super-resolution by stimulated emission depletion (STED) microscopy achieving a resolution of ~50 nm laterally and ~130 nm axially has not yet frequently been applied in plant cell research due to the required specific sample preparation and stable dye staining. Single-molecule localization microscopy (SMLM) including photoactivated localization microscopy (PALM) has not yet been widely used, although this nanoscopic technique allows even the detection of single molecules. In this study, we compared protein imaging within metaphase chromosomes of barley via conventional wide-field and confocal microscopy, and the sub-diffraction methods SIM, STED, and SMLM. The chromosomes were labeled by DAPI (4′,6-diamidino-2-phenylindol), a DNA-specific dye, and with antibodies against topoisomerase IIα (Topo II), a protein important for correct chromatin condensation. Compared to the diffraction-limited methods, the combination of the three different super-resolution imaging techniques delivered tremendous additional insights into the plant chromosome architecture through the achieved increased resolution.

scholarly journals Camera-based localization microscopy optimized with calibrated structured illumination

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Martin Schmidt ◽  
Adam C. Hundahl ◽  
Henrik Flyvbjerg ◽  
Rodolphe Marie ◽  
Kim I. Mortensen
Keyword(s):  
Data Analysis ◽  
Super Resolution ◽  
Wide Field ◽  
Structured Illumination ◽  
Uniform Illumination ◽  
Localization Microscopy ◽  
Localization And Tracking ◽  
Field Imaging ◽  
Wide Field Imaging ◽  
Localization Information

AbstractUntil very recently, super-resolution localization and tracking of fluorescent particles used camera-based wide-field imaging with uniform illumination. Then it was demonstrated that structured illuminations encode additional localization information in images. The first demonstration of this uses scanning and hence suffers from limited throughput. This limitation was mitigated by fusing camera-based localization with wide-field structured illumination. Current implementations, however, use effectively only half the localization information that they encode in images. Here we demonstrate how all of this information may be exploited by careful calibration of the structured illumination. Our approach achieves maximal resolution for given structured illumination, has a simple data analysis, and applies to any structured illumination in principle. We demonstrate this with an only slightly modified wide-field microscope. Our protocol should boost the emerging field of high-precision localization with structured illumination.

scholarly journals Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158884 ◽  
Author(s):  
Leila Nahidiazar ◽  
Alexandra V. Agronskaia ◽  
Jorrit Broertjes ◽  
Bram van den Broek ◽  
Kees Jalink
Keyword(s):  
Super Resolution ◽  
Localization Microscopy ◽  
Imaging Conditions

scholarly journals Effects of Central Tube on Shape of Modal Duct of a Helicoid in a Simple Expansion Chamber

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Daniel Omondi Onyango ◽  
Robert Kinyua ◽  
Abel Nyakundi Mayaka
Keyword(s):  
Wave Propagation ◽  
Acoustic Wave ◽  
Plane Wave ◽  
Acoustic Pressure ◽  
Three Dimensional ◽  
The Other ◽  
Expansion Chamber ◽  
Central Tube ◽  
Simple Expansion ◽  
Plane Wave Propagation

The shape of the modal duct of an acoustic wave propagating in a muffling system varies with the internal geometry. This shape can be either as a result of plane wave propagation or three-dimensional wave propagation. These shapes depict the distribution of acoustic pressure that may be used in the design or modification of mufflers to create resonance at cut-off frequencies and hence achieve noise attenuation or special effects on the output of the noise. This research compares the shapes of acoustic duct modes of two sets of four pitch configurations of a helicoid in a simple expansion chamber with and without a central tube. Models are generated using Autodesk Inventor modeling software and imported into ANSYS 18.2, where a fluid volume from the complex computer-aided-design (CAD) geometry is extracted for three-dimensional (3D) analysis. Mesh is generated to capture the details of the fluid cavity for frequency range between 0 and 2000Hz. After defining acoustic properties, acoustic boundary conditions and loads were defined at inlet and outlet ports before computation. Postprocessed acoustic results of the modal shapes and transmission loss (TL) characteristics of the two configurations were obtained and compared for geometries of the same helical pitch. It was established that whereas plane wave propagation in a simple expansion chamber (SEC) resulted in a clearly defined acoustic pressure pattern across the propagation path, the distribution in the configurations with and without the central tube depicted three-dimensional acoustic wave propagation characteristics, with patterns scattering or consolidating to regions of either very low or very high acoustic pressure differentials. A difference of about 80 decibels between the highest and lowest acoustic pressure levels was observed for the modal duct of the geometry with four turns and with a central tube. On the other hand, the shape of the TL curve shifts from a sinusoidal-shaped profile with well-defined peaks and valleys in definite multiples of π for the simple expansion chamber, while that of the other two configurations depended on the variation in wavelength that affects the location of occurrence of cut-on or cut-off frequency. The geometry with four turns and a central tube had a maximum value of TL of about 90 decibels at approximately 1900Hz.

scholarly journals Super-resolution binding activated localization microscopy through reversible change of DNA conformation

Nucleus ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 182-189 ◽  
Author(s):  
Aleksander Szczurek ◽  
Udo Birk ◽  
Hans Knecht ◽  
Jurek Dobrucki ◽  
Sabine Mai ◽  
...  
Keyword(s):  
Super Resolution ◽  
Dna Conformation ◽  
Reversible Change ◽  
Localization Microscopy

Aberration Characterization and Correction in Super-Resolution Localization Microscopy

2017 ◽  
Vol 37 (3) ◽  
pp. 0318004
Author(s):  
赵泽宇 Zhao Zeyu ◽  
张肇宁 Zhang Zhaoning ◽  
黄振立 Huang Zhenli
Keyword(s):  
Super Resolution ◽  
Localization Microscopy

scholarly journals Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

2019 ◽  
Vol 16 (5) ◽  
pp. 387-395 ◽  
Author(s):  
Daniel Sage ◽  
Thanh-An Pham ◽  
Hazen Babcock ◽  
Tomas Lukes ◽  
Thomas Pengo ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Localization Microscopy ◽  
Fight Club ◽  
2D And 3D ◽  
Single Molecule Localization Microscopy
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