Nanoparticle‐stabilized encapsulation of borneol and citral: Physicochemical characteristics, storage stability, and enhanced antibacterial activities

2021 ◽  
Vol 86 (10) ◽  
pp. 4554-4565
Author(s):  
Wen Wang ◽  
Zhihui Ren ◽  
Li Wang ◽  
Ying Cai ◽  
Hang Ma ◽  
...  
2020 ◽  
Vol 11 (3) ◽  
pp. 10703-10715

The objective of this study was to evaluate the impact of the addition of oligofructose or polydextrose (2 g/100 g) and Lacticaseibacillus casei (2 g/L) on the quality parameters of grape juices during storage (7 oC/28 days in polyethylene terephthalate flasks). The addition of probiotics or prebiotics did not alter the physicochemical characteristics, texture properties, and storage stability of the grape juices; however, they changed the color and/or turbidity. Oligofructose and polydextrose did not show a protective effect on the probiotic, but the products showed probiotic viability higher than 106 CFU/mL. The grape juice with oligofructose had similar acceptance to the control juice. The addition of polydextrose or reduced probiotic acceptance in most of the evaluated attributes. All grape juices presented scores higher than 6 on a 9-point hedonic scale and an acceptability index higher than 70%. It is possible to produce potentially synbiotic grape juices with suitable quality parameters, probiotic survival, and sensory acceptance.


Genome ◽  
2020 ◽  
Author(s):  
Yaru Gao ◽  
Xinyang Du ◽  
Huanhuan Li ◽  
Ying Wang

Due to the specific properties of the marine environment, marine microorganisms have exclusive physicochemical characteristics that are different from those of terrestrial microorganisms, which can produce various secondary metabolites (SMs) with considerable structural diversity and biological activity. In this study, three strains of coepiphytic Aspergillus with potential antibacterial activities, A7 (Aspergillus flavus), B27 (Aspergillus flavipes) and R12 (Aspergillus sydowii), were isolated from the South China Sea. Via the Illumina MiSeq sequencing platform, the genomes of the three strains were sequenced, and genome comparison showed the highest diversity of the biosynthetic gene clusters (BGCs) in A7. Meanwhile, a comparison of physiological and genomic characteristics between A7 and other Aspergillus flavus strains demonstrated the superior environmental adaptability of A7, which is apparently consistent with the genetic richness of BGCs. By assigning reads to known BGCs, putative BGCs were allocated in A7 that corresponded to various SMs, including naphthopyrone, pyranonigrin E, cyclopiazonic acids, etc. Based on gene homology analysis, we surmise that a region is involved in the biosynthesis of ustiloxin-like RiPPs, a less thoroughly studied SM in fungi. Our results provide genetic information for the investigation of marine Aspergillus sp., which may help to elucidate their chemical diversity and adaptive strategies.


2010 ◽  
Vol 97-101 ◽  
pp. 979-982 ◽  
Author(s):  
Lu Xia ◽  
You Shou Zhang ◽  
Jin Huang

Influences of additives on the physicochemical characteristics and technologies of binder are researched, and a new compound phosphate heat-cured foundry binder coded B8M15 is developed. The binder is made from environmental friendly inorganic materials such as phosphoric acid, aluminum hydroxide, B-ion additive and Mg-ion additive. Laboratory results indicate that combined effect of B-ion additive and Mg-ion additive can improve the storage stability of the binder and its sand mold or core, as well as they can increase the dry strength of its sand mold or core from 0.77MPa to 2.51MPa. This binder has low gas evolution and good collapsibility.


The emergence and global spread of multi antibiotic-resistant bacteria underscored the need to find new alternative antimicrobial candidates. Graphene quantum dots have received tremendous attention as promising new microbicidal agents owing to their ease of production, excellent physicochemical properties and high biosafety. In this chapter, the synthesis and physicochemical characteristics of graphene quantum dots are reviewed. A recent research progress on their antibacterial activities and the reaction mechanisms are also discussed. Lastly, an outlook on future development of effective graphene quantum dots was suggested with the goal of addressing current limitation and motivating further research on this promising area.


2020 ◽  
Vol 62 ◽  
pp. 96-107
Author(s):  
Zahra Faraji ◽  
Jahanshir Shakarami ◽  
Jaleh Varshosaz ◽  
Shahriar Jafari

This study attempted to evaluate the physicochemical characteristics of essential oil nanoliposomes from three plants by two preparation methods including thin film sonication and heating. Also, the durability of fumigation toxicity of the essential oil nanoliposome and pure essential oil of Mentha pulegium against Tribolium castaneum Herbst adults were compared. The smallest mean size of the nanoliposome was prepared by the thin film sonication method (P<0.05). The encapsulation efficiency of the nanoliposome was higher than 96%. Nanoliposmes prepared by using two methods showed high stability during storage. The release profile of essential oils at the heating method showed that the essential oil diffusion from the nanoliposome was in the following order: Mentha pulegium > Ferula gummosa > Zattaria multiflora. These findings indicate that both preparation methods had the same effect on the formation of nanoliposomes, their characteristics, and their storage stability. The durability of fumigation toxicity of the nanoliposome M. pulegium essential oil had a significant effect on mortality of T. castaneum adults in comparison with pure essential oil of this plant. The result showed that nanoliposome encapsulation of M. pulegium essential oil significantly increased fumigation toxicity against T. castaneum. Therefore, essential oil encapsulation into a nanoliposome carrier can potentially be used in pest management.


2021 ◽  
Vol 10 (1) ◽  
pp. 14-24
Author(s):  
Hannah O Akinrotohun ◽  
Ayobami O Oyedele ◽  
Oluwatoyin A Igbeneghu ◽  
Lara O Orafidiya

The aims of this study were to determine the emulsifying properties of Theobroma cacao pod husk ash (CPHA) methanolic extract combined with shea butter and explore the antibacterial activities and physicochemical characteristics of resulting emulsions toward the development of a topical antibacterial lotion formulation for shaving bumps treatment. The ash resulting from combustion of pod husks of freshly harvested ripe cocoa fruits was extracted with methanol and the extract evaporated to dryness. Shea butter was also extracted by traditional method from kernels from the shea tree. These natural-source materials were combined with pharmaceutical ingredients (buffer, viscosity enhancer, preservative) to develop fluid emulsion formulations. Stability characteristics (droplet size, viscosity, creaming, and pH) of the formulations were evaluated as well as their antibacterial activities against microorganisms isolated from after-shave bump swabs of adult male volunteers and against reference organisms; in order to select product(s) of best qualities suitable as shaving bumps medication. The prototype formulations exhibited suitable physicochemical properties and demonstrated inhibitory activities against several isolated shaving bump microbes and the reference organisms namely, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. Two formulations were finally selected as having physicochemical and antibacterial qualities most suitable for shaving bumps therapy, which contained shea butter (20%), citrate buffer (5%), and parabens (0.3%), prepared using 5% CPHA extract solution with and without methyl cellulose (2%), respectively. The novel shea butter-incorporated emulsion-lotion formulations of CPHA extract provide a useful therapeutic option of topical medication for the treatment of shaving bumps in men.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tamer M. Tamer ◽  
Maysa M. Sabet ◽  
Ahmed M. Omer ◽  
Eman Abbas ◽  
Alaa I. Eid ◽  
...  

AbstractHemorrhage is the major hindrance over the wound healing, which triggers microbial infections and might provoke traumatic death. Herein, new hemostatic and antibacterial PVA/Kaolin composite sponges were crosslinked using a freeze-thawing approach and boosted by penicillin–streptomycin (Pen-Strep). Physicochemical characteristics of developed membranes were analyzed adopting Fourier transformed infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), a thermal gravimetric analyzer (TGA), and differential scanning calorimetry (DSC). Furthermore, the impacts of kaolin concentrations on porosity, swelling behavior, gel fraction, and degradation of the membranes were investigated. SEM analyses revealed a spongy-like structure of hydrogels associated with high dispersion of kaolin inside PVA matrix. The thermal characteristics of PVA/Kaolin were significantly ameliorated compared to the prime PVA. Moreover, the results exhibited significant variations of swelling performance, surface roughness and pore capacity due to the alterations of kaolin contents. Besides, the adhesive strength ability was manifestly enhanced for PVA-K0.1 sponge. Biomedical evaluations including antibacterial activity, blood clotting index and thrombogenicity of the membranes were studied. The contact of PVA/Kaolin to blood revealed notable augmentation in blood clotting. Furthermore, the incorporation of kaolin into PVA presented mild diminution in antibacterial activities. Moreover, PVA/Kaolin composites illustrated no cellular toxicity towards fibroblast cells. These remarkable features substantiate that the PVA-K0.1 sponge could be applied as a multifunctional wound dressing.


Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3012
Author(s):  
Is Fatimah ◽  
Gani Purwiandono ◽  
Habibi Hidayat ◽  
Suresh Sagadevan ◽  
Sheikh Ahmad Izaddin Sheikh Mohd Ghazali ◽  
...  

The present study reported biofabrication of flower-like SnO2 nanoparticles using Pometia pinnata leaf extract. The study focused on the physicochemical characteristics of the prepared SnO2 nanoparticles and its activity as photocatalyst and antibacterial agent. The characterization was performed by XRD, SEM, TEM, UV-DRS and XPS analyses. Photocatalytic activity of the nanoparticles was examined on bromophenol blue photooxidation; meanwhile, the antibacterial activity was evaluated against Klebsiella pneumoniae, Escherichia coli Staphylococcus aureus and Streptococcus pyogenes. XRD and XPS analyses confirmed the single tetragonal SnO2 phase. The result from SEM analysis indicates the flower like morphology of SnO2 nanoparticles, and by TEM analysis, the nanoparticles were seen to be in uniform spherical shapes with a diameter ranging from 8 to 20 nm. SnO2 nanoparticles showed significant photocatalytic activity in photooxidation of bromophenol blue as the degradation efficiency reached 99.93%, and the photocatalyst exhibited the reusability as the degradation efficiency values were insignificantly changed until the fifth cycle. Antibacterial assay indicated that the synthesized SnO2 nanoparticles exhibit an inhibition of tested bacteria and showed a potential to be applied for further environmental and medical applications.


2020 ◽  
Author(s):  
Marco Drexelius ◽  
André Reinhardt ◽  
Joshua Grabeck ◽  
Tom Cronenberg ◽  
Frank Nitsche ◽  
...  

Multidrug resistant (MDR) bacteria have adapted to most clinical antibiotics and are a growing threat to human health. One promising type of candidates for the everlasting demand of new antibiotic compounds constitute antimicrobial peptides (AMPs). These peptides act against different types of microbes by permeabilizing pathogen cell membranes, whereas being harmless to mammalian cells. Contrarily, another class of membrane-active peptides, namely cell-penetrating peptides (CPPs), is known to translocate in eukaryotic cells without substantially affecting the cell membrane. Since CPPs and AMPs share several physicochemical characteristics, we hypothesized if we can rationally direct the activity of a CPP towards antimicrobial activity. Herein, we describe the screening of a synthetic library, based on the CPP sC18, including structure-based design to identify the active residues within a CPP sequence and to discover novel AMPs with high activity. Peptides with increased hydrophobicity were tested against various bacterial strains, and hits were further optimized leading to four generations of peptides, with the last also comprising fluorinated amino acid building blocks. Interestingly, beside strong antibacterial activities, we also detected activity in cancer cells, while non-cancerous cells remained unharmed. The results highlight our new candidates, particularly those from generation 4, as a valuable and promising source for the development of future therapeutics with antibacterial activity and beyond.


Sign in / Sign up

Export Citation Format

Share Document