Assessment of the anti‐rheumatoid arthritis activity of Gastrodia elata (tian‐ma) and Radix aconitic lateralis preparata (fu‐zi) via network pharmacology and untargeted metabolomics analyses

2021 ◽  
Author(s):  
Jie Yang ◽  
Yu Zhang ◽  
Wei‐Hong Li ◽  
Bu‐Fa Guo ◽  
Qi‐Lun Peng ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Untargeted Metabolomics ◽  
Network Pharmacology ◽  
Gastrodia Elata ◽  
Rheumatoid Arthritis Activity

scholarly journals The Anti-RA Activities of a Couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata, Explored by Untargeted Metabolomics and Network Pharmacology.

2020 ◽  
Author(s):  
Jie Yang ◽  
Xiang-Chun SHEN ◽  
Wei-Hong LI ◽  
Guang-Zhong FAN ◽  
Bu-Fa GUO ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Endothelial Function ◽  
Unsaturated Fatty Acids ◽  
Untargeted Metabolomics ◽  
Network Pharmacology ◽  
Gastrodia Elata ◽  
Fundamental Process ◽  
Key Genes ◽  
Combined Strategy ◽  
Anti Inflammation

Abstract Background The couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata (GERA), is an established formula extensively used in Chinese medicine for treating rheumatoid arthritis (RA). However, the anti-RA mechanisms of GERA are still unclear. This paper aims to explore the anti-RA mechanisms of GERA by a combined strategy of untargeted metabolomics and network pharmacology. Methods Three water extracts were prepared as propotions: Gastrodia Elata compared with Radix Aconitic Lateralis (w/w): 1:1, 3:2, or 2:3. The untargeted metabolomics were executed with UPLC-MS. The metabolites were annotated and identified by Human metabolome database (HMDB) and Lipid maps database. Finally, key genes and pathways related to anti-RA activities of GERA were mined by network pharmacology. Results The untargeted metabolomics profile displayed that four differentially expressed metabolites were involved in isoflavonoid biosynthesis and biosynthesis of unsaturated fatty acids (p < 0.05). Among these differential metabolites, the essential ingredients of GERA were linoleic acid, daidzein, and daidzin. The principal targets of anti-RA activities of GERA were IL-6, TNF, VEGFA, TP53, CASP3 and PTGS2. Thirty anti-RA targets of GERA were majorly belonged to pathways response for anti-inflammation, endothelial function and apoptosis, suggesting the fundamental process of RA treatment. Conclusion The anti-RA activities of GERA were based on the inhibition of inflammation and regulation of endothelial function and apoptosis.

Reliability of Seromucoid as an Indicator of Rheumatoid Arthritis Activity

1972 ◽  
Vol 1 (1) ◽  
pp. 2-4 ◽  
Author(s):  
Irena Zimmermann-górska ◽  
Marja Routavaara ◽  
Jukka Martio ◽  
Zimmermann-górska
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Arthritis Activity

scholarly journals AB0110 DETERMINING THE AMOUNT OF COPPER–CONTAINING PROTEIN, ITS BIOCHEMICAL AND IMMUNOLOGIC ACTIVITY IN RHEUMATOID ARTHRITIS PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1083.2-1084
Author(s):  
O. Rusanova ◽  
A. Trofimenko ◽  
N. Emelyanov ◽  
O. Emelyanova
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Enzyme Immunoassay ◽  
Oxidase Activity ◽  
Mean Value ◽  
Hospital Treatment ◽  
Diagnostic Agent ◽  
Immunologic Activity ◽  
Rheumatoid Arthritis Activity

Background:Production of antibodies to ceruloplasmin (CP) in rheumatoid arthritis is an issue that has not been studied well enough. It was not by chance that this copper–containing alpha 2-glycorpoteid of blood plasma showing multienzymatic properties was chosen as an object of investigation. Data on the content and activity of CP in the blood of rheumatoid arthritis patients are contradictory, which has to do with different approaches to selection of patients and different measuring methods.Objectives:Improving diagnosis of rheumatoid arthritis by determination of antibodies to CP as well as its amount and enzymatic activity.Methods:We studied the serum from 30 apparently healthy individuals, and 108 rheumatoid arthritis patients. Antibodies to CP were determined by enzyme immunoassay using immobilized granulated antigen preparations (modification by Gontar et al, 2002). The amount of CP was determined by enzyme immunoassay according to the method of I.S. Kuzmina et al (1991) using commercial diagnostic agent manufactured by Mechnikov Research Institute for Vaccines and Sera.Results:Enzyme immunoassay showed a mean level of CP antibodies in donor sera of 0,020±0,006 optical density units. The level of normal values of specific antibodies determined as M±2σ included an extinction value in the range 0 – 0,086. The mean value of oxidase activity and the amount of CP in healthy people was 716±26,3 and 921±32 ng/ml, correspondingly. In the process of study we revealed a reliable increase in CP antibody count, the activity and amount of CP in patients with rheumatoid arthritis while in all cases the parameters under study correlated with the degree of disease activity (p<0,05): at activity degree I CP antibodies were 0,098±0,011; CP activity was 954±48,1; CP amount was 1292±73,4. At activity degree II CP antibodies were 0,138±0,007; CP activity was 1163±39,6; CP amount was 1763±69,3. At activity degree III, CP antibodies were 0,182±0,015; CP activity was 1368±89,5; CP amount was 1794±102,8. After a course of hospital treatment was completed, we noted a reliable decrease in the activity and amount of CP (at degree I of rheumatoid arthritis activity p<0,001, at degree II of rheumatoid arthritis activity p<0,01for both parameters; at degree III, p<0,05) compared with baseline findings. A decrease in CP antibodies shows decelerated dynamics, especially in patients with pronounced disease activity, which indicates severe disorders in the immunity that cannot be cured completely within 30 – 40 days of hospital treatment course.Conclusion:Determination of CP antibodies, as well as quantitative content of CP and its oxidase activity can serve as indicators of the activity of rheumatoid arthritis, as well as an accessory criterion of the effectiveness of administered therapy.Disclosure of Interests:None declared

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