A Study on Preserving Endometrial Glandular Architecture during Preparation using BD SurePath™ Liquid based cytology reagents: Cellular Fixation with Preservative Fluid Requires at least 18 Hours

Cytopathology ◽  
2021 ◽  
Author(s):  
Takeshi Nishikawa ◽  
Hisae Suzuki ◽  
Mao Takeuchi ◽  
Shigenobu Tatsumi ◽  
Yuma Tachibana ◽  
...  
Keyword(s):  
Liquid Based Cytology

scholarly journals Profiles of Human Papillomavirus Detection of the Multinucleated Cells in Cervical Smears

2021 ◽  
Vol 9 (8) ◽  
pp. 1575
Author(s):  
Kaori Okayama ◽  
Toshiyuki Sasagawa ◽  
Koji Teruya ◽  
Mizue Oda ◽  
Masahiko Fujii ◽  
...  
Keyword(s):  
Pap Smear ◽  
Morphological Changes ◽  
Human Papillomaviruses ◽  
Multinucleated Cells ◽  
Hpv Genotypes ◽  
Atypical Cells ◽  
Intraepithelial Lesion ◽  
Liquid Based Cytology ◽  
Cervical Cells ◽  
High Risk Hpv

Many genotypes of human papillomaviruses (HPVs) may lead to morphological changes in cells, resulting in various atypical cells, such as multinucleated cells (MNCs) and koilocytes, in the cervix. However, the relationships between the profiles of HPV genotypes and MNCs are not exactly known. Thus, this study comprehensively profiles the HPV genotypes in MNCs using a microdissection method. HPV genotypes and MNCs were detected in 651 cases with an abnormal Pap smear by liquid-based cytology. Specific HPV genotypes were also detected, including HPV16, 34, and 56, which might be associated with MNCs. This result suggests that the high-risk HPV genotypes, such as HPV16 and 56, are associated with the atypical changes in MNC morphology from normal cervical cells. The results also show that MNCs may be a predictor of squamous intraepithelial lesion.

Rapid Cell Transfer by Means of Nylon Mesh to Improve Cellular Diagnosis: The Role of Immunocytochemistry

2021 ◽  
pp. 1-6
Author(s):  
Satoshi Morito ◽  
Takao Nitanda ◽  
Ryuko Tsukamoto ◽  
Shingo Kamoshida ◽  
Hiroshi Yasui ◽  
...  
Keyword(s):  
Needle Aspiration ◽  
Turnaround Time ◽  
Body Cavity ◽  
Cell Transfer ◽  
Special Equipment ◽  
Nylon Mesh ◽  
Cytological Smear ◽  
Liquid Based Cytology ◽  
Transfer Method

Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3–40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.

scholarly journals Performance of the DNA-Citoliq liquid-based cytology system compared with conventional smears

Cytopathology ◽  
2006 ◽  
Vol 17 (2) ◽  
pp. 86-93 ◽  
Author(s):  
V. A. F. Alves ◽  
A. Castelo ◽  
A. L. Filho ◽  
M. R. Vianna ◽  
E. Taromaru ◽  
...  
Keyword(s):  
Liquid Based Cytology

Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

2014 ◽  
Vol 58 (6) ◽  
pp. 522-532 ◽  
Author(s):  
Shuyue Ren ◽  
Charalambos Solomides ◽  
Rossitza Draganova-Tacheva ◽  
Marluce Bibbo
Keyword(s):  
Liquid Based Cytology

Endometrial Adenocarcinoma in SurePath™ Cervical Samples: Cytological Features Revisited

2016 ◽  
Vol 60 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Nalini Gupta ◽  
John Crossley ◽  
Nick Dudding ◽  
John H.F. Smith
Keyword(s):  
Phase 1 ◽  
Endometrial Adenocarcinoma ◽  
Nuclear Chromatin ◽  
Phase 2 ◽  
Cervical Lesions ◽  
Two Phase ◽  
Endometrial Cells ◽  
Phase Study ◽  
Cytological Features ◽  
Liquid Based Cytology

Objective: The cytomorphological criteria of malignant endometrial lesions in cervical samples are less well described than those of cervical lesions. We wished to investigate if there were features in SurePath™ liquid-based cytology samples that would facilitate more accurate differentiation between benign and malignant endometrial cells. Study Design: This was a two-phase study, with a review of all SurePath™ samples reported as endometrial adenocarcinoma (n = 42) evaluating 12 cytological features in the first phase. In phase 2 (test set), all initial cases plus an additional 83 cases were reviewed using these 12 cytological features to predict the outcome. Results: Out of 12 cytological features evaluated in phase 1 (training set), nuclear chromatin pattern, apoptotic bodies and tingible body macrophages were found to be the most significant features determining malignant histological outcome. These 12 cytological features were re-evaluated in phase 2 (n = 125). Of 125 cases, 54 had a benign and 71 had a malignant or premalignant histological outcome, with a positive predictive value of 56.8%. Conclusion: Granular nuclear chromatin, tingible body macrophages and apoptosis in the background are the most significant factors in determining whether endometrial cells present in cervical samples represent malignancy or are benign. Using these features, relatively accurate predictions of endometrial pathology can be made.

Performance of self-sampled HPV test in comparison with liquid based cytology

2014 ◽  
Vol 177 ◽  
pp. 72-76 ◽  
Author(s):  
Sarah Igidbashian ◽  
Sara Boveri ◽  
Davide Radice ◽  
Chiara Casadio ◽  
Noemi Spolti ◽  
...  
Keyword(s):  
Hpv Test ◽  
Liquid Based Cytology
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