Predictors of Growth of Escherichia coli on Lab Coats as Part of Hospital‐Acquired Infection Transmission through Healthcare Personnel Attire

Abstract Introduction Bacteria in most environments exist as communities of sessile cells in a selfproduced polymeric matrix known as biofilms. Biofilms are responsible for more than 80% of infections, including urinary tract infections (UTI). UTI is the most common hospital acquired infection, caused mainly by Escherichia coli (E.coli). E. coli can readily form biofilm in such infections, specially in the presence of indwelling urinary catheter. It’s difficult to eradicate bacteria in biofilms, since they are shielded from the host defense mechanisms as phagocytes and antibodies, as well as antibiotics. Searching for alternative or adjuvant substances for prevention and eradication of biofilm associated infections are therefore urgently needed. Aim of the work Studying the efficacy of the trans-cinnamaldehyde (TC) for preventing E. coli biofilm formation. Materials and methods Thirty isolates of E.coli were obtained from urine samples. To test the effect of TC on E.coli biofilm formation and preformed biofilms, microtitre plates (MTP) were inoculated with the isolated E.coli and were treated with different concentrations of TC and incubated at 37° C. A colorimetric assay was used to assess biofilm inhibition and inactivation and optical densities (OD) were compared before and after adding different TC concentrations. Results The mean OD of the isolated E.coli biofilms was 1.3 and significantly decreased when mixed with TC different concentrations. TC had high activity in inhibition of preformed E.coli biofilms, where no biofilm was detected on MTP treated with 1.25% and 1.5% TC. Conclusion TC inhibited the biofilm forming ability of E.coli isolates could fully inactivate formed biofilms, suggesting its possibility to be used as an anti-biofilm agent or adjuvant in preventing and treating UTI caused by biofilm producing E.coli.

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Transcriptional response of Mar, Sox and Rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection

10.21203/rs.2.20996/v1 ◽

2020 ◽

Author(s):

Shiela Chetri ◽

Bhaskar Jyoti Das ◽

Deepshikha Bhowmik ◽

Debadatta Dhar Chanda ◽

Atanu Chakravarty ◽

...

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Concentration Gradient ◽

Efflux Pump ◽

Transcriptional Response ◽

Multiple Antibiotic Resistance ◽

Pump System ◽

Hospital Acquired Infection ◽

Outer Membrane Porin ◽

Hospital Acquired

Abstract Objective The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress. Results 12 isolates over-expressing AcrAB-TolC efflux pump system and showing reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, overexpression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems.

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Transcriptional response of Mar, Sox and Rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection

10.21203/rs.2.20996/v2 ◽

2020 ◽

Author(s):

Shiela Chetri ◽

Bhaskar Jyoti Das ◽

Deepshikha Bhowmik ◽

Debadatta Dhar Chanda ◽

Atanu Chakravarty ◽

...

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Concentration Gradient ◽

Efflux Pump ◽

Transcriptional Response ◽

Multiple Antibiotic Resistance ◽

Pump System ◽

Hospital Acquired Infection ◽

Outer Membrane Porin ◽

Hospital Acquired

Abstract Objective The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress. Results 12 isolates over-expressing AcrAB-TolC efflux pump system and showing reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, overexpression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems.

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Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di Instalasi Gawat Darurayt RSAD Robert Wolter Mongisidi Manado

Jurnal e-Biomedik ◽

10.35790/ebm.4.2.2016.14608 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Sitti N. Gobel ◽

Fredine E.S. Rares ◽

Heriyannis Homenta

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Emergency Room ◽

Medical Equipment ◽

Ambient Air ◽

Aerobic Bacteria ◽

Bacillus Sp ◽

Enterobacter Agglomerans ◽

Hospital Acquired Infection ◽

Hospital Acquired

Abstract: Nosocomial infection or Hospital Acquired infection (HAI) is caused by bacteria, parasites or viruses in hospitals and occurs after ≥48 hours of hospital admission. Emergency room is te starting place procedures and or medical measures so that the room IGD became one of the places that produces a lot of waste of medical procedures performed and as one of the places most likely to transmit the infection. This study was aimed to determine the sources and patterns of aerobic bacteria that could potentially cause nosocomial infections: in the walls, floors, medical equipment, and ambient air of Emergency Room Robert Wolter Mongisidi Hospital, Manado. This was a descriptive prospective study with a cross sectional design. Samples were bacteria in the walls, floors, medical equipment, and the ambient air of the room IGD in the emergency room, Robert Wolter Mongisidi Hospital, Manado. The results showed that of the 29 samples taken, one sample did not contain any bacteria. There were eight types of bacteria found: Bacillus sp (33%), Staphylococcus sp (27%) Enterobacter agglomerans (13%), Escherichia coli (10%), Streptococcus sp (10%), Serratia marcescens (3%), Neisseria sp (2%) and Klebsiella pneumoniae (2%). Conclusion: The most common bacteria was Bacillus sp.Keywords: IGD, nosocomial infections, patterns of aerobic bacteria Abstrak: Infeksi nosokomial atau Hospital Acquired infection (HAI) merupakan infeksi yang disebabkan oleh bakteri, parasit atau virus di rumah sakit, terjadi ≥ 48 jam sejak masuk rumah sakit. Ruangan IGD merupakan tempat awal pasien mendapatkan prosedur dan atau tindakan medis sehingga ruangan IGD menjadi salah satu tempat yang banyak menghasilkan limbah hasil prosedur dan tindakan medis yang dilakukan serta salah satu tempat yang paling mungkin menularkan infeksi. Penelitian ini bertujuan untuk mengetahui sumber dan pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial: di dinding, lantai, peralatan medis, dan udara di ruang IGD pada ruang gawat darurat medik RSAD Robert Wolter Mongisidi Manado. Jenis penelitian ialah deskriptif prospektif dengan desain otong lintang. Sampel penelitian ialah bakteri pada dinding, lantai, peralatan medis, dan udara di ruang gawat darura tmedik RSAD Robert Wolter Mongisidi Manado. Hasil penelitian memperlihatkan dari 29 sampel yang diambil 1 sampel tidak terdapat bakteri. Terdapat 8 jenis bakteri yang ditemukan yaitu: Bacillus sp (33%), Staphylococcus sp (27%), Enterobacter agglomerans (13%), Escherichia coli (10%), Streptococcus sp (10%), Serratia marcescens (3%), Neisseria sp (2%) dan Klebsiella Pneumoniae (2%). Simpulan: Bakteri yang paling banyak ditemukan ialah Bacillus sp. Kata kunci: IGD, infeksi nosokomial, pola bakteri aerob

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Assessment of bacterial contamination of toilets and bathroom doors handle/knobs at Daeyang Luke hospital

Pharmaceutical and Biological Evaluations ◽

10.26510/2394-0859.pbe.2017.31 ◽

2017 ◽

Vol 4 (4) ◽

pp. 193 ◽

Cited By ~ 6

Author(s):

Frank Ngonda

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacterial Contamination ◽

Sterile Water ◽

Hospital Acquired Infection ◽

Bacterial Contaminant ◽

Surgical Ward ◽

Hospital Acquired ◽

Infectious Organism

Objective: The aim of the study was to determine the level of bacterial contamination of door handles/knobs of toilets and bathrooms at Daeyang Luke Hospital.Methods: Fomite can be described as a non-living object capable of carrying infectious organism and it is considered as one of the major source of spread of hospital acquired infection. In this study, door handles/knobs were swabbed with sterile swab stick moistened with sterile water. The content was evenly transferred to nutrient agar petric dish and then incubated for 24 hours at 37˚C. The study was done between the months of May to August 2016.Results: The results of the study showed that during the period of study there was 41.6% bacterial contamination with the female medical and surgical ward toilets being more contaminated as compared to staff toilets. Private room bathrooms were more contaminated during the period of study as compared to the staff room bathrooms that were less contaminated. Among the bacterial contaminant isolated and identified, Staphylococcus aureus had the highest prevalence followed by Escherichia coli, Pseudomonas aeruginosa, Citrobacter species and Klebsiella pneumoniae respectively.Conclusions: Therefore, from the study, it can be concluded that general public needs observe hand hygiene and use of quality disinfection procedures in order to reduce hospital acquired infection.

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POLA BAKTERI PADA PENDERITA INFEKSI SALURAN KEMIH DI BLU RSUP PROF. dr. R. D. KANDOU MANADO

Jurnal e-Biomedik ◽

10.35790/ebm.1.1.2013.4605 ◽

2013 ◽

Vol 1 (1) ◽

Author(s):

Shirby A. Ch. Sumolang ◽

John Porotu’o ◽

Standy Soeliongan

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Medical Emergency ◽

Mortality Pattern ◽

Hospital Acquired Infection ◽

Wide Scale ◽

A Prospective Study ◽

Hospital Acquired

Abstract: The most common hospital-acquired infection is urinary tract infection (UTI) resulting in significant morbidity and occasional mortality. Pattern of germs especially bacteries that cause UTI will contribute to the success of the treatment of UTI. The wide scale spectrum of the causative organisms, and least of clinical trials that have been done are make the forming of the selected antimicroba that will used for the therapy of UTI more difficult. The purpose of this study was to determine the pattern of germs that cause UTIs, which are important in improving the quality of health care in the Prof. Dr. R. D. Kandou Manado Hospital. This study uses a prospective study that analyzed descriptively on 30 urinary samples in November until December 2012 at Installation of Medical Emergency Care of Prof. Dr. R. D. Kandou Manado Hospital. Results of this research showed the highest incidence of UTI is in the age group 50-59 years. Women suffer from UTI more often than men. In this research, Escherichia coli is the most common microorganisms that cause UTI. Keywords: bacteries, pattern germs, urinary tract infection. Abstrak: Infeksi Saluran Kemih (ISK) adalah infeksi yang paling umum didapat di RS yang mengakibatkan angka morbiditas dan mortalitas yang signifikan. Pola kuman penyebab ISK akan berperan penting dalam keberhasilan pengobatan ISK. Bervariasinya penyebab ISK, luasnya spektrum organisme yang menjadi penyebab, serta sedikitnya uji klinis yang telah dilaksanakan, mempersulit penyusunan antimikroba pilihan yang dapat digunakan dalam terapi ISK. Tujuan dari penelitian ini adalah untuk mengetahui pola kuman khususnya bakteri penyebab ISK, yang merupakan hal penting dalam meningkatkan kualitas pelayanan kesehatan di RSUP. Prof. Dr. R. D. Kandou Manado. Penelitian ini menggunakan metode penelitian prospektif yang dianalisis secara deskriptif pada 30 sampel urin selama bulan November sampai Desember 2012 di Instalasi Rawat Darurat Medik RSUP Prof. dr. R. D. Kandou Manado. Hasil dari penelitian ini menunjukkan bahwa insidensi ISK tertinggi adalah pada kelompok umur 50-59 tahun. Perempuan lebih sering menderita ISK daripada laki-laki. Dari penelitian ini, Escherichia coli merupakan mikroorganisme tersering yang menyebabkan ISK. Kata Kunci: Bakteri, Infeksi saluran kemih, Pola kuman.

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Hospital-Acquired SARS-Cov-2 Infections in Patients: Inevitable Conditions or Medical Malpractice?

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18020489 ◽

2021 ◽

Vol 18 (2) ◽

pp. 489

Author(s):

Rosario Barranco ◽

Luca Vallega Bernucci Du Tremoul ◽

Francesco Ventura

Keyword(s):

Medical Malpractice ◽

Literature Search ◽

Point Of View ◽

Healthcare Personnel ◽

Hospital Treatment ◽

Public Health Issue ◽

Hospital Acquired Infection ◽

Legal Implications ◽

Scientific Papers ◽

Hospital Acquired

Despite numerous measures to contain the infection and limit its spread, cases of SARS-CoV-2 infections acquired in hospitals have been reported consistently. In this paper, we will address issues of hospital-acquired COVID-19 in hospitalized patients as well as medico-legal implications. After having conducted a literature search, we will report on papers on hospital-acquired SARS-CoV-2 infections. Ten scientific papers were selected and considered suitable for further analysis. According to several reports, the SARS-CoV-2 hospital-acquired infection rate is 12–15%. Hospital-acquired COVID-19 represents a serious public health issue, which is a problem that could create reluctance of patients to seek hospital treatment for fear of becoming infected. Healthcare personnel should do all that is necessary to address the problem and prevent further spreading, such as rigorous compliance with all procedures for containing the spread. From a medical-legal point of view, multiple aspects must be considered in order to understand whether the infection is a result of “malpractice” or an inevitable condition.

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Isolation of post operative hospital acquired infections in a private hospital of Bangladesh and identification of clonal relatedness of E. coli by using Pulse filed gel electrophoresis

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v6i2.19369 ◽

2012 ◽

Vol 6 (2) ◽

pp. 7-10

Author(s):

Mohammad Murshed ◽

Sabeena Shahnaz ◽

Md. Abdul Malek

Keyword(s):

Private Hospital ◽

Isolation And Identification ◽

College Hospital ◽

Hospital Acquired Infection ◽

E Coli ◽

Hospital Acquired Infections ◽

Medical College ◽

Clonal Relatedness ◽

Hospital Acquired ◽

Dressing Materials

Isolation and identification of post operative hospital acquired infection was carried out from July 2008 to December 2008 in Holy Family Red Crescent Medical College Hospital (private hospital). The major pathogen of wound infection was E. coli. A total; of 120 samples were collected from the surrounding environment of post operative room like floor, bed sheets, instruments, dressing materials, catheter, nasogastric and endotracheal tube. E. coli (40%) was the predominant organism followed by S. aureus (24%). DNA fingerprinting analysis using pulsed field gel electreopheresis of XbaI restriction digested genomic DNA showed that clonal relatedness between the two clinical nd environmental isolates were 100%.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19369 Bangladesh J Med Microbiol 2012; 06(02): 7-10

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Hospital-acquired sepsis with Escherichia coli – a case analysis

Infectio ro ◽

10.26416/inf.53.1.2018.1611 ◽

2018 ◽

Vol 1 (53) ◽

pp. 26

Author(s):

Irina Brumboiu ◽

Irina Cazacu

Keyword(s):

Escherichia Coli ◽

Case Analysis ◽

Hospital Acquired

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