scholarly journals Quantitative Microarray-Based DNA-DNA Hybridization Assay for Measuring Genetic Distances among Bacterial Species and Its Application to the Identification of FamilyEnterobacteriaceae

2005 ◽  
Vol 49 (3) ◽  
pp. 255-263 ◽  
Author(s):  
Makoto Amano ◽  
Kiyofumi Ohkusu ◽  
Koji Kusaba ◽  
Hironori Ikeda ◽  
Zenzo Nagasawa ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Bacterial Species ◽  
Genetic Distances ◽  
Hybridization Assay

Peptoniphilus Colimassiliensis sp. nov. and Peptoniphilus Urinimassiliensis sp. nov., Two New Species Isolated From Humans

2021 ◽  
Author(s):  
Babacar Mbaye ◽  
Cheikh Ibrahima LO ◽  
Niokhor Dione ◽  
Sarah Benabdelkader ◽  
Maryam Tidjani Alou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dna Hybridization ◽  
Kidney Transplant Recipient ◽  
Bacterial Species ◽  
Hexadecanoic Acid ◽  
Anaerobic Conditions ◽  
New Members ◽  
Two New Species ◽  
Type Strains ◽  
Gram Positive Cocci

Abstract Strains Marseille-P3761 and Marseille-P3195 are representatives of two bacterial species isolated from human specimens. Strain Marseille-P3761 was isolated from the stool of a healthy volunteer, while strain Marseille-P3915 was cultivated from the urine of a kidney transplant recipient. Both strains are anaerobic Gram-positive cocci bacteria. Both are catalase-negative and oxidase-negative and grow optimally at 37°C in anaerobic conditions. They also metabolize carbohydrates such as galactose, glucose, fructose, and glycerol. The major fatty acids were hexadecanoic acid for both strains, Marseille-P3761 (38%) and Marseille-P3195 (31%). The highest DNA-DNA hybridization values of Marseille-P3761 and Marseille-P3195 strains when compared to their closest phylogenetic relatives were 52.3% and 56.4%, respectively. The morphological, biochemical, phenotypic and genomic characteristics strongly support that these strains are new members of the Peptoniphilus genus. Thus, we suggest that strains Marseille-P3761 (CSUR P3761 = CCUG71569) and Marseille-P3195 (CSUR P3195 = DSM 103468) are the type strains of two new Peptoniphilus species, for which we propose the names Peptoniphilus colimassiliensis sp. nov. and Peptoniphilus urinimassiliensis sp. nov., respectively.

A rapid chemiluminescent DNA hybridization assay for the detection ofChlamydia trachomatis

1989 ◽  
Vol 4 (1) ◽  
pp. 357-366 ◽  
Author(s):  
J. M. Clyne ◽  
J. A. Running ◽  
M. Stempien ◽  
R. S. Stephens ◽  
H. Akhavan-Tafti ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Hybridization Assay ◽  
Ofchlamydia Trachomatis ◽  
Detection Ofchlamydia Trachomatis

scholarly journals Antimicrobial-Resistant Enteric Bacteria from Dairy Cattle

2006 ◽  
Vol 73 (1) ◽  
pp. 156-163 ◽  
Author(s):  
Ashish A. Sawant ◽  
Narasimha V. Hegde ◽  
Beth A. Straley ◽  
Sarah C. Donaldson ◽  
Brenda C. Love ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Dna Hybridization ◽  
Bacterial Species ◽  
Dairy Herd ◽  
Enteric Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Predominant Species ◽  
Lactating Dairy Cattle

ABSTRACT A study was conducted to understand the descriptive and molecular epidemiology of antimicrobial-resistant gram-negative enteric bacteria in the feces of healthy lactating dairy cattle. Gram-negative enteric bacteria resistant to ampicillin, florfenicol, spectinomycin, and tetracycline were isolated from the feces of 35, 8, 5, and 42% of 213 lactating cattle on 74, 39, 9, 26, and 82% of 23 farms surveyed, respectively. Antimicrobial-resistant gram-negative bacteria accounted for 5 (florfenicol) to 14% (tetracycline) of total gram-negative enteric microflora. Nine bacterial species were isolated, of which Escherichia coli (87%) was the most predominant species. MICs showing reduced susceptibility to ampicillin, ceftiofur, chloramphenicol, florfenicol, spectinomycin, streptomycin, and tetracycline were observed in E. coli isolates. Isolates exhibited resistance to ampicillin (48%), ceftiofur (11%), chloramphenicol (20%), florfenicol (78%), spectinomycin (18%), and tetracycline (93%). Multidrug resistance (≥3 to 6 antimicrobials) was seen in 40% of E. coli isolates from healthy lactating cattle. Of 113 tetracycline-resistant E. coli isolates, tet(B) was the predominant resistance determinant and was detected in 93% of isolates, while the remaining 7% isolates carried the tet(A) determinant. DNA-DNA hybridization assays revealed that tet determinants were located on the chromosome. Pulsed-field gel electrophoresis revealed that tetracycline-resistant E. coli isolates (n = 99 isolates) belonged to 60 subtypes, which is suggestive of a highly diverse population of tetracycline-resistant organisms. On most occasions, E. coli subtypes, although shared between cows within the herd, were confined mostly to a dairy herd. The findings of this study suggest that commensal enteric E. coli from healthy lactating cattle can be an important reservoir for tetracycline and perhaps other antimicrobial resistance determinants.

Rapid antiviral DNA-DNA hybridization assay for human cytomegalovirus

1990 ◽  
Vol 28 (3) ◽  
pp. 293-298 ◽  
Author(s):  
Wayne M. Dankner ◽  
David Scholl ◽  
Sylvia C. Stanat ◽  
Michael Martin ◽  
Robert L. Sonke ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Dna Hybridization ◽  
Hybridization Assay

Rapid detection of Staphylococcus aureus via a sensitive DNA hybridization assay based on a long-lifetime luminescent europium marker

2011 ◽  
Vol 175 (1-2) ◽  
pp. 105-112 ◽  
Author(s):  
Min Ruan ◽  
Cheng-Gang Niu ◽  
Guang-Ming Zeng ◽  
Pin-Zhu Qin ◽  
Xiao-Yu Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dna Hybridization ◽  
Rapid Detection ◽  
Hybridization Assay ◽  
Long Lifetime

scholarly journals ‘Candidatus Borrelia texasensis’, from the American dog tick Dermacentor variabilis

2005 ◽  
Vol 55 (2) ◽  
pp. 685-693 ◽  
Author(s):  
Tao Lin ◽  
Lihui Gao ◽  
Andreas Seyfang ◽  
James H. Oliver
Keyword(s):  
Dna Hybridization ◽  
Rapd Analysis ◽  
Rflp Analysis ◽  
Dermacentor Variabilis ◽  
Genetic Distances ◽  
Rrna Gene ◽  
Relapsing Fever ◽  
Tick Feeding ◽  
Culture Collections ◽  
Banding Patterns

TXW-1, a Borrelia strain isolated in March 1998 from an adult male Dermacentor variabilis tick feeding on a coyote from Webb county, Texas, USA, was characterized by using randomly amplified polymorphic DNA (RAPD) analysis, RFLP and sequence analysis of flaB and rrs (16S rRNA gene), DNA–DNA hybridization analysis, SDS-PAGE and Western blotting with mAbs. It shows different banding patterns in RFLP analysis of flaB and forms distinct branches in phylogenetic analysis derived from flaB and rrs genes. It differs from other borreliae based on the banding patterns obtained by RAPD analysis. This strain contains a small, 38-kDa endoflagellar protein. DNA–DNA hybridization experiments revealed that the levels of DNA reassociation between TXW-1 and previously described relapsing fever borreliae were 38·64 % (Borrelia turicatae), 38·40 % (Borrelia parkeri), 7·39 % (Borrelia hermsii) and 18·30 % (Borrelia coriaceae). However, the level of DNA relatedness between B. parkeri and B. turicatae was 78·78 %. Sequence analyses of flaB and rrs genes indicate that the similarities of nucleotide sequences among TXW-1 and B. turicatae or B. parkeri are less than that between B. turicatae and B. parkeri, and that the genetic distances among TXW-1 and B. turicatae or B. parkeri are greater than that between B. turicatae and B. parkeri. TXW-1 lacks an ospC gene. Electron microscope observations showed that this spirochaete had different morphological structures compared to previously described relapsing fever borreliae. All the results obtained from the above-mentioned analyses indicate that TXW-1 is different from other described Borrelia species and that it represents a novel species of Borrelia. We have been unable to revive frozen cultures and so can not meet the requirements of the Bacteriological Code to deposit viable type material at two different culture collections. Therefore we use the Candidatus designation; based on these results, the species ‘Candidatus Borrelia texasensis' is proposed.

Fluorescence coupled in-capillary DNA hybridization assay and its application to identification of DNA point mutation

2016 ◽  
Vol 237 ◽  
pp. 106-112 ◽  
Author(s):  
Jianhao Wang ◽  
Yuqin Qin ◽  
Shi-Wen Jiang ◽  
Li Liu ◽  
Yao Lu ◽  
...  
Keyword(s):  
Point Mutation ◽  
Dna Hybridization ◽  
Hybridization Assay
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