In vitro amino acid absorption using hydrolysed sardine muscle or soybean meal at different intestinal regions of the Pacific bluefin tuna (Thunnus orientalis)

2011 ◽  
Vol 17 (3) ◽  
pp. e789-e797 ◽  
Author(s):  
E. MARTÍNEZ-MONTAÑO ◽  
E. PEÑA ◽  
M.T. VIANA
Aquaculture ◽  
2021 ◽  
Vol 530 ◽  
pp. 735769
Author(s):  
Takao Hayashida ◽  
Kentaro Higuchi ◽  
Kazuharu Nomura ◽  
Junpei Konishi ◽  
Yukinori Shimada ◽  
...  

2006 ◽  
Vol 23 (5) ◽  
pp. 443-448 ◽  
Author(s):  
Yutaka Kawakami ◽  
Manabu Seoka ◽  
Shigeru Miyashita ◽  
Hidemi Kumai ◽  
Hiromi Ohta

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1705
Author(s):  
Gyoungsik Kang ◽  
Kwang-Min Choi ◽  
Dong-Hee Cho ◽  
Min-Soo Joo ◽  
Min-Jin Heo ◽  
...  

The consumption of fish and shellfish worldwide is steadily increasing, and tuna is a particularly valuable fish species. However, infection caused by Kudoa spp. is causing problems in many fish including the Pacific bluefin tuna (Thunnus orientalis), and there is much controversy about the association of these infections with foodborne disease. In this study, using haematological and histological analyses of the blood and internal organs (liver, spleen, kidney, heart, stomach, intestine, gill, and muscle) of Pacific bluefin tuna cultured in South Korea, infection with Myxosporea was first identified, and molecular biological analysis was conducted. In this study, Kudoa hexapunctata was finally identified. The Pacific bluefin tunas analysed in this study did not show any gross pathology lesions, such as visible cysts and/or myoliquefaction, of infection with this species. The histological analytical results can provide guidelines for the identification of K. hexapunctata. In the case of K. hexapunctata-induced infection, unlike other countries, such as Japan, there have been no reports in South Korea, and this study is the first to detect K. hexapunctata infection in Pacific bluefin tuna cultured in South Korea. The correlation between K. hexapunctata and food poisoning is not yet clear, however, it is thought that continuous observation of its infection is necessary.


1997 ◽  
Vol 272 (4) ◽  
pp. G815-G821 ◽  
Author(s):  
K. A. Barada ◽  
S. S. Dika ◽  
S. F. Atweh ◽  
N. E. Saade ◽  
C. F. Nassar

It has recently been shown that capsaicin inhibits alanine absorption in rat jejunum via mechanisms that involve intestinal capsaicin-sensitive primary afferent (CSPA) fibers. This study provides further evidence that the effect of capsaicin is neurally mediated and demonstrates that CSPA fibers regulate Na+-dependent amino acid absorption. In vivo, basal alanine absorption in rats neonatally treated with capsaicin was reduced by 35% below control. Furthermore, intraluminal perfusion of 400 microM capsaicin reduced jejunal alanine absorption by 31% in sham rats but had no significant effect in rats neonatally treated with capsaicin. In vitro, capsaicin significantly reduced uptake of alanine and proline by jejunal strips but had no effect on uptake of lysine. Tetrodotoxin (0.2 microM) partially blocked the effects of capsaicin but did not itself affect alanine absorption. Capsaicin reduced unidirectional mucosal-to-serosal alanine (1 mM) influx by 33%, an effect that becomes significant after 5 min of preincubation with capsaicin. Neonatal capsaicin treatment reduced basal alanine influx in jejunal strips by 37%; however, preincubation of these strips with capsaicin had no significant effect. Kinetic analysis of alanine steady-state uptake and influx by jejunal strips incubated with capsaicin revealed that capsaicin reduced the Na+-dependent component of alanine influx into intestinal epithelial cells. Long-term sensory denervation by capsaicin also decreased the Na+-dependent component of alanine absorption. These data suggest that intestinal capsaicin-sensitive primary afferent fibers regulate Na+-dependent amino acid absorption.


Aquaculture ◽  
2006 ◽  
Vol 261 (1) ◽  
pp. 392-406 ◽  
Author(s):  
Ståle Refstie ◽  
Anne Marie Bakke-McKellep ◽  
Michael H. Penn ◽  
Anne Sundby ◽  
Karl D. Shearer ◽  
...  

2011 ◽  
Vol 77 (4) ◽  
pp. 615-621 ◽  
Author(s):  
Amal Biswas ◽  
Biswajit K. Biswas ◽  
Junichi Ito ◽  
Osamu Takaoka ◽  
Norishige Yagi ◽  
...  

1981 ◽  
Vol 241 (2) ◽  
pp. G176-G181
Author(s):  
R. S. Green ◽  
R. G. MacDermid ◽  
R. L. Scheig ◽  
J. J. Hajjar

The acute effect of ethanol on amino acid absorption across the in vivo rat intestine was studied using single-pass continuous perfusion and recirculation techniques. The single-pass steady-state perfusion was used to examine the effect on the entire small intestine and recirculation perfusion to examine the effect on short intestinal segments and to limit ethanol absorption. Unlike the in vitro findings of other investigators, ethanol does not cause inhibition of net amino acid absorption in vivo unless the alcohol is perfused in 2 M or higher concentrations. The inhibition that is observed at these concentrations is very likely due to severe injury and shedding of intestinal cells as evidenced by an increased recovery of DNA in the perfusates. The findings suggest that acute ethanol administration, in concentrations that are comparable to those found in the upper intestines of humans after the ingestion of moderate doses of alcohol, does not have a prominent effect on amino acid absorption across the in situ rat intestine. Under these conditions, the ethanol inhibition of active absorption is masked by enhanced diffusion of the amino acids across the intestine.


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