Lack of Toll-like receptor 4 decreases lipopolysaccharide-induced bone resorption in C3H/HeJ mice in vivo

2008 ◽  
Vol 23 (3) ◽  
pp. 190-195 ◽  
Author(s):  
H. Nakamura ◽  
Y. Fukusaki ◽  
A. Yoshimura ◽  
C. Shiraishi ◽  
M. Kishimoto ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

scholarly journals Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 599
Author(s):  
Víctor Farré-Alins ◽  
Alejandra Palomino-Antolín ◽  
Paloma Narros-Fernández ◽  
Ana Belen Lopez-Rodriguez ◽  
Céline Decouty-Perez ◽  
...  
Keyword(s):  
Injury Severity ◽  
White Blood Cells ◽  
Serum Amyloid ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Important Link ◽  
Tlr4 Mrna ◽  
Serum Amyloid A1

Traumatic brain injury (TBI) is one of the leading causes of mortality and disability worldwide without any validated biomarker or set of biomarkers to help the diagnosis and evaluation of the evolution/prognosis of TBI patients. To achieve this aim, a deeper knowledge of the biochemical and pathophysiological processes triggered after the trauma is essential. Here, we identified the serum amyloid A1 protein-Toll-like receptor 4 (SAA1-TLR4) axis as an important link between inflammation and the outcome of TBI patients. Using serum and mRNA from white blood cells (WBC) of TBI patients, we found a positive correlation between serum SAA1 levels and injury severity, as well as with the 6-month outcome of TBI patients. SAA1 levels also correlate with the presence of TLR4 mRNA in WBC. In vitro, we found that SAA1 contributes to inflammation via TLR4 activation that releases inflammatory cytokines, which in turn increases SAA1 levels, establishing a positive proinflammatory loop. In vivo, post-TBI treatment with the TLR4-antagonist TAK242 reduces SAA1 levels, improves neurobehavioral outcome, and prevents blood–brain barrier disruption. Our data support further evaluation of (i) post-TBI treatment in the presence of TLR4 inhibition for limiting TBI-induced damage and (ii) SAA1-TLR4 as a biomarker of injury progression in TBI patients.

scholarly journals Micrococcus luteus Teichuronic Acids Activate Human and Murine Monocytic Cells in a CD14- and Toll-Like Receptor 4-Dependent Manner

2001 ◽  
Vol 69 (4) ◽  
pp. 2025-2030 ◽  
Author(s):  
Shuhua Yang ◽  
Shunji Sugawara ◽  
Toshihiko Monodane ◽  
Masahiro Nishijima ◽  
Yoshiyuki Adachi ◽  
...  
Keyword(s):  
Lipid A ◽  
Micrococcus Luteus ◽  
Dependent Manner ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Necrosis Factor Alpha ◽  
Monocytic Cells ◽  
Tnf Α

ABSTRACT Teichuronic acid (TUA), a component of the cell walls of the gram-positive organism Micrococcus luteus (formerlyMicrococcus lysodeikticus), induced inflammatory cytokines in C3H/HeN mice but not in lipopolysaccharide (LPS)-resistant C3H/HeJ mice that have a defect in the Toll-like receptor 4 (TLR4) gene, both in vivo and in vitro, similarly to LPS (T. Monodane, Y. Kawabata, S. Yang, S. Hase, and H. Takada, J. Med. Microbiol. 50:4–12, 2001). In this study, we found that purified TUA (p-TUA) induced tumor necrosis factor alpha (TNF-α) in murine monocytic J774.1 cells but not in mutant LR-9 cells expressing membrane CD14 at a lower level than the parent J774.1 cells. The TNF-α-inducing activity of p-TUA in J774.1 cells was completely inhibited by anti-mouse CD14 monoclonal antibody (MAb). p-TUA also induced interleukin-8 (IL-8) in human monocytic THP-1 cells differentiated to macrophage-like cells expressing CD14. Anti-human CD14 MAb, anti-human TLR4 MAb, and synthetic lipid A precursor IVA, an LPS antagonist, almost completely inhibited the IL-8-inducing ability of p-TUA, as well as LPS, in the differentiated THP-1 cells. Reduced p-TUA did not exhibit any activities in J774.1 or THP-1 cells. These findings strongly suggested that M. luteus TUA activates murine and human monocytic cells in a CD14- and TLR4-dependent manner, similar to LPS.

Pseudomonas aeruginosa Lipopolysaccharide Induces Osteoclastogenesis Through a Toll-Like Receptor 4 Mediated Pathway in Vitro and in Vivo

2007 ◽  
Vol 117 (5) ◽  
pp. 841-847 ◽  
Author(s):  
Lei Zhuang ◽  
Jae Y. Jung ◽  
Eric W. Wang ◽  
Patrick Houlihan ◽  
Lisette Ramos ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

scholarly journals Leukocyte- and Platelet-Derived Microvesicle Interactions following In Vitro and In Vivo Activation of Toll-Like Receptor 4 by Lipopolysaccharide

PLoS ONE ◽  
2011 ◽  
Vol 6 (9) ◽  
pp. e25504 ◽  
Author(s):  
Jing Xiong ◽  
Virginia M. Miller ◽  
Larry W. Hunter ◽  
Yunman Li ◽  
Muthuvel Jayachandran
Keyword(s):  
Toll Like Receptor ◽  
Toll Like Receptor 4

TNF-α promoter, Nod2 and toll-like receptor-4 polymorphisms and the in vivo and ex vivo response to endotoxin

Cytokine ◽  
2004 ◽  
Vol 26 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Emile F. Schippers ◽  
Cornelis van 't Veer ◽  
Sjaak van Voorden ◽  
Cerithsa A.E. Martina ◽  
Saskia le Cessie ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Tnf Α

194 A Non-Gluten Component of Wheat is a Strong Stimulator of Innate Immune Responses In Vitro and In Vivo and Acts via Toll Like Receptor 4

2010 ◽  
Vol 138 (5) ◽  
pp. S-36
Author(s):  
Yvonne Junker ◽  
Donatella Barisani ◽  
Daniel A. Leffler ◽  
Towia Libermann ◽  
Simon T. Dillon ◽  
...  
Keyword(s):  
Immune Responses ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

S100A8 Enhances Osteoclast-Mediated Bone Resorption in Experimental Antigen-Induced Arthritis Through Activation of Toll-Like Receptor 4

2012 ◽  
Vol 1 ◽  
pp. 20
Author(s):  
Lilyanne Grevers ◽  
Teun Vries ◽  
Thomas Vogl ◽  
Shahla AbdollahiRoodsaz ◽  
Annet Sloetjes ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

scholarly journals In Vivo Evidence for a Role of Toll-Like Receptor 4 in the Development of Intimal Lesions

Circulation ◽  
2002 ◽  
Vol 106 (15) ◽  
pp. 1985-1990 ◽  
Author(s):  
Aryan Vink ◽  
Arjan H. Schoneveld ◽  
Jelger J. van der Meer ◽  
Ben J. van Middelaar ◽  
Joost P.G. Sluijter ◽  
...  
Keyword(s):  
Toll Like Receptor ◽  
Toll Like Receptor 4
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