A capillary polymerase chain reaction for Salmonella detection from poultry meat

2007 ◽  
Vol 44 (1) ◽  
pp. 24-29 ◽  
Author(s):  
E. Gunaydin ◽  
A. Eyigor ◽  
K.T. Carli
Keyword(s):  
Polymerase Chain Reaction ◽  
Poultry Meat ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Morphological, Biochemical and Genotypic Analysis of Zoonotic Campylobacter jejuni Isolated from Chicken Meat Samples

2022 ◽  
Author(s):  
Sumedha Bobade ◽  
K. Vijayarani ◽  
K.G. Tirumurugaan ◽  
A. Thangavelu ◽  
S. Vairamuthu
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Phenotypic Characterization ◽  
Meat Production ◽  
Chain Reaction ◽  
Genotypic Analysis ◽  
Polymerase Chain ◽  
Meat Samples

Background: Campylobacter species are a leading cause of most important food-borne diarrhoeal illness worldwide while, poultry has been identified as a significant cause of Campylobacter infection in humans. C. jejuni is highly effective in colonizing chicken intestinal mucosa without causing any clinical manifestations and the consumption of poultry meat is the major source of transmission of bacteria to humans. Methods: The total of 19 chicken meat samples collected from retail markets in Chennai were screened by cultural examination, further subjected to phenotypic characterization using biochemical test and genotypic characterization using polymerase chain reaction assay targeting hip O and map A genes. Result: All the isolates showed growth on modified blood free charcoal cefoperazone deoxycholate agar media (mCCDA) and 18 (94.73%) samples showed typical morphological characteristics. The 12 (63.15%) isolates showed biochemical reactions positive. The results from polymerase chain reaction showed that 10 (83.33%) isolates were positive for C. jejuni. This study suggested that, it is essential to investigate the incidence of Campylobacter jejuni infection in poultry and the risk factors at all production stages of meat production to help reducing the disease in humans in terms of food safety.

Detection of chicken DNA in meat products sold in Moscow and Moscow region by polymerase chain reaction

2020 ◽  
pp. 26-29
Author(s):  
Zinaida N. Menshikova ◽  
◽  
Ksenia O. Lyubkina ◽  
Zelikha S. Devrishova ◽  
Anastasiya S. Preobrazhenskaya ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Analysis ◽  
Chemical Elements ◽  
Raw Materials ◽  
Meat Product ◽  
Meat Products ◽  
Moscow Region ◽  
Poultry Meat ◽  
Chain Reaction ◽  
Polymerase Chain

The most common type of falsification of animal products is the substitution of raw materials of more valuable types with less valuable ones, including poultry meat. This paper presents the results of identification of undeclared chicken DNA in meat products using real-time polymerase chain reaction to detect falsification of products sold in Moscow and the Moscow region. As a result of PCR research, chicken DNA (Gallus gallus) was found in six out of ten samples of meat products, but semi-quantitative analysis excluded one meat product, since the amount of the desired component was less than one percent. All ten samples were also subjected to organoleptic evaluation, physical and chemical studies, luminescent microscopy, and the determination of safety indicators (the number of chemical elements, pesticides, antibiotics, and radionuclides). The results of comprehensive research did not reveal any deviations. Thus, the method of polymerase chain reaction allows you to determine the type of raw materials in the composition of minced meat products, finely ground semi-finished products, including those subjected to heat treatment. To accurately confirm the presence of falsification of meat products detected by PCR-RV, not only qualitative analysis, but also quantitative analysis is necessary.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

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