11Microbial dysbiosis in a mouse model of atopic dermatitis mimics shifts in human microbiome and correlates with the key pro‐inflammatory cytokines IL‐4, IL‐33 and TSLP

Key Points Differential expression of WNT ligands in patients with septic shock and a mouse model of endotoxemia correlates with inflammatory cytokines. WNT ligands and WNT/β-catenin signaling positively regulate lipopolysaccharide-induced pro-inflammatory cytokines without impairing IL-10.

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P069 CD73 promotes colitis-associated tumourigenesis in mice

Journal of Crohn s and Colitis ◽

10.1093/ecco-jcc/jjz203.198 ◽

2020 ◽

Vol 14 (Supplement_1) ◽

pp. S170-S170

Author(s):

X Wu ◽

X Liu ◽

N Lan ◽

X Zheng ◽

Y Chen ◽

...

Keyword(s):

Weight Loss ◽

Mouse Model ◽

Rna Sequencing ◽

Inflammatory Cytokines ◽

Adenosine Receptors ◽

Tumour Size ◽

Body Weight Loss ◽

Model Group ◽

Adenosine Receptor Agonist ◽

Pro Inflammatory Cytokines

Abstract Background Patients with inflammatory bowel disease (IBD) are at a higher risk of developing colitis-associated colorectal cancer. The aim of the present study was to investigate the role of CD73 in IBD-associated tumourigenesis. Methods A mouse model of colitis-associated tumourigenesis (CAT) induced by azoxymethane and dextran sulphate sodium (AOM/DSS) was successfully constructed. Model mice were injected with CD73 inhibitor or adenosine receptor agonist. Colon length, body weight loss and tumour formation were assessed macroscopically. Measurement of inflammatory cytokines and RNA sequencing on colon tissues were performed. Results Inhibition of CD73 by adenosine 5′-(α,β-methylene) diphosphate (APCP) suppressed the severity of CAT with attenuated weight loss, longer colons, lower tumour number and smaller tumour size when compared with the model group. On the other hand, activation of adenosine receptors using 1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-β-d-ribofuranuronamide (NECA) exacerbated CAT. Histological assessment indicated that inhibition of CD73 reduced while activation of adenosine receptors exacerbated the histological damage of the colon compared with the model group. Increased expression of pro-inflammatory cytokines (tumour necrosis factor-α and interleukin-6) in colonic tissue was detected in the NECA group. According to the results of RNA sequencing, potential oncogenes such as ALOX15, Bcl2l15 and Nat8l were found to be downregulated in the APCP group and upregulated in the NECA group compared with the model group. Conclusion Therefore, inhibition of CD73 attenuated IBD-associated tumourigenesis, while activation of adenosine receptors exacerbated tumourigenesis in a C57BL/6J mouse model. This effect may be associated with the expression of pro-inflammatory cytokines and the regulation of ALOX15, Bcl2l15 and Nat8l.

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Increase in the secretion of pro-inflammatory cytokines by peritoneal leucocytes in a genetic mouse model of obesity and metabolic syndrome

Proceedings of The Nutrition Society ◽

10.1017/s0029665113000839 ◽

2013 ◽

Vol 72 (OCE1) ◽

Author(s):

N. M. de Castro ◽

J. Cruces ◽

M. De La Fuente

Keyword(s):

Metabolic Syndrome ◽

Mouse Model ◽

Inflammatory Cytokines ◽

Genetic Mouse Model ◽

Pro Inflammatory Cytokines

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Increase in the secretion of pro-inflammatory cytokines by spleen leucocytes in a genetic mouse model of obesity and metabolic syndrome

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2012.08.316 ◽

2012 ◽

Vol 53 ◽

pp. S151

Author(s):

N.M. De Castro ◽

J. Cruces ◽

M. De la Fuente⁎

Keyword(s):

Metabolic Syndrome ◽

Mouse Model ◽

Inflammatory Cytokines ◽

Genetic Mouse Model ◽

Pro Inflammatory Cytokines

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Effects of Acupuncture on 1-Chloro-2,4-dinitrochlorobenzene-Induced Atopic Dermatitis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/982095 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Ji-Yeun Park ◽

Hi-Joon Park ◽

You Yeon Choi ◽

Mi Hye Kim ◽

Seung-Nam Kim ◽

...

Keyword(s):

Atopic Dermatitis ◽

Contact Dermatitis ◽

Allergic Contact Dermatitis ◽

Inflammatory Cytokines ◽

Acupuncture Treatment ◽

Skin Inflammation ◽

Serum Ige ◽

Local Point ◽

Allergic Contact ◽

Pro Inflammatory Cytokines

Though the effects of acupuncture in atopic dermatitis have been proven in clinical studies, its mechanism remains unclear. In this study, we investigate the effectiveness and mechanism of action for acupuncture treatment on the LI11 meridian point for treatment of allergic contact dermatitis. BALB/c mice received 1-chloro-2,4-dinitrobenzene (DNCB) application to induce skin inflammation. Acupuncture treatment on LI11 significantly inhibited cutaneous hyperplasia, serum IgE levels, and expression of proinflammatory cytokine (IL-4, IL-8, and TNF-α) mRNA and NF-κB, ERK1/2, JNK, and p38 proteins. Acupuncture treatment of local points also inhibited cutaneous hyperplasia and serum IgE levels; however, it was not effective in regulating proinflammatory cytokines and proteins. In addition, LI11 treatment is more effective at reducing serum IgE levels and pro-inflammatory cytokines and proteins than local point treatment. These results suggest that acupuncture treatment is effective in alleviating allergic contact dermatitis by reducing pro-inflammatory cytokines and proteins.

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