Assessment of population distribution, conservation status and in vitro propagation of Cymbidium whiteae King & Pantl. in the Sikkim Himalaya, India

2022 ◽  
Author(s):  
Ram Pal ◽  
P. Kiran Babu ◽  
Deepak Rai ◽  
Ravi Kishore Pamarthi ◽  
Duniya Ram Singh
Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1657
Author(s):  
Nqobile P. Hlophe ◽  
Adeyemi O. Aremu ◽  
Karel Doležal ◽  
Johannes Van Staden ◽  
Jeffrey F. Finnie

In Africa and Asia, members of the genus Brachystelma are well-known for their diverse uses, especially their medicinal and nutritional values. However, the use of many Brachystelma species as a valuable resource is generally accompanied by the concern of over-exploitation attributed to their slow growth and general small size. The aim of the current study was to establish efficient micropropagation protocols for three Brachystelma species, namely Brachystelma ngomense (endangered), Brachystelma pulchellum (vulnerable) and Brachystelma pygmaeum (least concern), as a means of ensuring their conservation and survival. This was achieved using nodal segments (~10 mm in length) as the source of explants in the presence of different concentrations of three cytokinins (CK) namely N6-benzyladenine (BA), isopentenyladenine (iP) and meta-topolin riboside (mTR), over a period of 6 weeks. The highest (25 µM) concentration of cytokinin treatments typically resulted in significantly higher shoot proliferation. However, each species differed in its response to specific CK: the optimal concentrations were 25 µM mTR, 25 µM iP and 25 µM BA for Brachystelma ngomense, Brachystelma pulchellum and Brachystelma pygmaeum, respectively. During the in vitro propagation, both Brachystelma ngomense and Brachystelma pygmaeum rooted poorly while regenerated Brachystelma pulchellum generally lacked roots regardless of the CK treatments. Following pulsing (dipping) treatment of in vitro-regenerated shoots with indole-3-butyric acid (IBA), acclimatization of all three Brachystelma species remained extremely limited due to poor rooting ex vitro. To the best of our knowledge, the current protocols provide the first successful report for these Brachystelma species. However, further research remains essential to enhance the efficiency of the devised protocol.


Author(s):  
Armine Abrahamyan

Armenia has a strong interest in examining the biodiversity of native plant species and assessing their conservation status. Anthropogenic threats to this biodiversity such as overpopulation, deforestation and urbanization have simultaneously hindered research and increased the need for it. Only limited information on the genetic biodiversity, population location, structure and size, and conservation status of most of these species is, however, at this time available. During 2006-2009, field studies were conducted to re-locate populations of wild Melissa officinalis L. on the basis of historical (i.e. herbarium voucher) records, and to discover new populations. The growth, phenological and habitat characteristics of the plant, and overall populations size were assessed. GPS map of present and past population distribution were created. This research provided baseline data that can be used for the development of further ex situ and in vitro strategies to conserve unique genotypes, as well as to assess the sustainability of wild populations with regard to the IUCN Red Book Criteria, of this important medicinal and culinary species in Armenia.


Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
M Gangopadhyay ◽  
R Bhattacharya ◽  
D Chakraborty ◽  
S Bhattacharya ◽  
A Mitra ◽  
...  

2018 ◽  
pp. 47-52

Epimedium elatum (Morren & Decne) of family Berberidaceace is a rare perennial medicinal plant, endemic to high altitude forests of Northwestern Himalayas in India. Ethnobotanically, it has been used as an ingredient for treatment of bone-joint disorders, impotence and kidney disorders in Kashmir Himalayas. Phytochemically, it is rich in Epimedin ABC and Icariin; all of these have been demonstrated to possess remarkable biological activities like PDE-5 inhibition (treatment of erectile dysfunction), anticancer, antiosteoporosis antioxidant and antiviral properties. The present investigation reports its traditional usage, comprehensive distribution and conservation status from twenty ecogeographical regions in Kashmir Himalayas, India. The species was reported from Gurez valley for the first time. Numerous threats like excessive grazing, deforestration, habitat fragmentation, tourism encroachment, landslides and excessive exploitation have decreased its natural populations in most of the surveyed habitats. Consequently, its existence may become threatened in near future if timely conservation steps are not taken immediately by concerned stakeholders involved in medicinal plant research. Moreover, use of plant tissue culture techniques is recommended for development of its in vitro propagation protocols. Therefore, introduction of this medicinal plant in botanical gardens, protected sites and development of monitoring programmes are needed for its immediate conservation in Northwestern Himalayas, India.


Green Farming ◽  
2020 ◽  
Vol 11 (2-3) ◽  
pp. 130
Author(s):  
VIJAYAKUMARI N. ◽  
P. GHOSH ◽  
Y.B. LAHANE ◽  
K.P. FISKE

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