scholarly journals Measurement and modification of free calcium transients in frog skeletal muscle fibres by a metallochromic indicator dye.

1983 ◽  
Vol 343 (1) ◽  
pp. 161-196 ◽  
Author(s):  
L Kovacs ◽  
E Rios ◽  
M F Schneider
Keyword(s):  
Skeletal Muscle ◽  
Calcium Transients ◽  
Free Calcium ◽  
Muscle Fibres ◽  
Frog Skeletal Muscle ◽  
Skeletal Muscle Fibres ◽  
Indicator Dye

Measurement of calcium transients in frog muscle by the use of arsenazo III

1977 ◽  
Vol 198 (1131) ◽  
pp. 201-210 ◽  
Keyword(s):  
Calcium Release ◽  
Calcium Transients ◽  
Arsenazo Iii ◽  
Free Calcium ◽  
Muscle Fibres ◽  
Bathing Solution ◽  
Intracellular Injection ◽  
Calcium Indicator ◽  
Skeletal Muscle Fibres ◽  
Indicator Dye

Intracellular injection of the calcium indicator dye arsenazo III was used to measure the calcium transients occurring during depolarization of frog skeletal muscle fibres. A quantitative estimate of the rise in intracellular free calcium during a twitch was made, and the relation between membrane potential and calcium release was examined. The results also indicate that calcium in the bathing solution plays no important part in the generation of calcium transients during single twitches.

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