scholarly journals Isolation Rate and Susceptibilities of Candida Species from Blood, Vascular Catheter, Urine and Stool

2010 ◽  
Vol 84 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Masato TASHIRO ◽  
Hinako MURAKAMI ◽  
Sadako YOSHIZAWA ◽  
Kazuhiro TATEDA ◽  
Keizo YAMAGUCHI
Author(s):  
Hoai Do Ngoc

From 43.574 fluid nasopharynx speciments of  the chidren inpatient under six we isolated total 21.769 types bacteria with isolation rate : 49.95%. In which the highest isolation rate for H. influenza, S. pneumoniae and M. catarrhalis were 13,94%; 7,11%; 1,43% respectively. Antimicrobial susceptibility testing shown all the types of  for H. influenza, S. pneumoniae and M. catarrhalis good susses to Fosphomycine, S. pneumoniae and M. catarrhalis good susses to Imipenem, H. influenza good susses to Azithromycine, S. pneumoniae good susses to Penicilline and Piperacilline, M. catarrhalis good susses to Tobramycine and Ofloxacine. All of  H. influenza, S. pneumoniae and M. catarrhalis were reported resistance to Tri/Sulpha, Chloramphenicol, Erythromycine in high rate.


2020 ◽  
Vol 28 (2) ◽  
pp. 243-250 ◽  
Author(s):  
Yu Chen ◽  
Jin Cheng ◽  
Yu Jiang ◽  
Keji Liu

AbstractIn this paper, we propose a novel dynamical system with time delay to describe the outbreak of 2019-nCoV in China. One typical feature of this epidemic is that it can spread in the latent period, which can therefore be described by time delay process in the differential equations. The accumulated numbers of classified populations are employed as variables, which is consistent with the official data and facilitates the parameter identification. The numerical methods for the prediction of the outbreak of 2019-nCoV and parameter identification are provided, and the numerical results show that the novel dynamic system can well predict the outbreak trend so far. Based on the numerical simulations, we suggest that the transmission of individuals should be greatly controlled with high isolation rate by the government.


Cancers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 535
Author(s):  
Anouk E. Hentschel ◽  
Rianne van den Helder ◽  
Nienke E. van Trommel ◽  
Annina P. van Splunter ◽  
Robert A. A. van Boerdonk ◽  
...  

In urogenital cancers, urine as a liquid biopsy for non-invasive cancer detection holds great promise for future clinical application. Their anatomical position allows for the local shedding of tumor DNA, but recent data indicate that tumor DNA in urine might also result from transrenal excretion. This study aims to assess the origin of tumor-associated DNA in the urine of 5 bladder and 25 cervical cancer patients. Besides natural voided urine, paired urine samples were collected in which contact with the local tumor was circumvented to bypass local shedding. The latter concerned nephrostomy urine in bladder cancer patients, and catheter urine in cervical cancer patients. Methylation levels of GHSR, SST, and ZIC1 were determined using paired bladder tumor tissues and cervical scrapes as a reference. Urinary methylation levels were compared to natural voided urine of matched controls. To support methylation results, mutation analysis was performed in urine and tissue samples of bladder cancer patients. Increased methylation levels were not only found in natural voided urine from bladder and cervical cancer patients, but also in the corresponding nephrostomy and catheter urine. DNA mutations detected in bladder tumor tissues were also detectable in all paired natural voided urine as well as in a subset of nephrostomy urine. These results provide the first evidence that the suitability of urine as a liquid biopsy for urogenital cancers relies both on the local shedding of tumor cells and cell fragments, as well as the transrenal excretion of tumor DNA into the urine.


2017 ◽  
Vol 25 (4) ◽  
pp. 635-640 ◽  
Author(s):  
Viktor Janz ◽  
Andrej Trampuz ◽  
Carsten F. Perka ◽  
Georgi I. Wassilew

PEDIATRICS ◽  
1980 ◽  
Vol 66 (1) ◽  
pp. 50-55
Author(s):  
Margaret A. Keller ◽  
Rouben Aftandelians ◽  
James D. Connor

One hundred patients with clinical pertussis were studied to determine the etiology of pertussis syndrome. Forty-two (42%) of the patients had either Bordetella pertussis or Bordetella parapertussis isolated from the nasopharynx. In an additional 36 (36%) patients, B pertussis was isolated from the nasopharynx of the associated index case or family contact case. Thus, Bordetella was isolated from 78 (78%) of the patients or from their immediate family group. Of the 22 culture-negative patients residing in culture-negative families, 12 had serologic evidence of Bordetella infection and another was from a family group in which two members were seropositive. Therefore, 91 patients (91%) had bacteriologic or serologic evidence of Bordetella infection themselves or within their families. Viral cultures were obtained on 75 of the patients. Adenoviruses were isolated from 33% of those with positive cultures for B pertussis and from 14% of those with negative cultures. In the group without direct or indirect, bacteriologic or serologic evidence of Bordetella infection, the adenoviral isolation rate (13%) was not significantly different from the adenoviral isolation rate (33%) in patients with a positive bacterial culture. These data do not support a role for adenovirus alone in causing pertussis syndrome.


Author(s):  
Mohamed El-Diasty ◽  
◽  
Rana El-Said ◽  
Adel Abdelkhalek ◽  
◽  
...  

Brucellosis is an endemic disease among livestock and humans in Egypt. Sheep are the most common type of livestock ruminant in Egypt and considered the fundamental etiology for spreading and maintaining B. melitensis either in human being or animal populations. In the current study, we investigated the seroprevalence of brucellosis in sheep herds reared in Bilqase, one of the biggest cities at Dakahlia governorate in Egypt's Delta region. In total, 610 sheep from seven herds were investigated. Anti-Brucella antibodies were detected in 48 (7.8%) samples tested by Buffered Acidified Plate Antigen Test (BAPAT), in 44 (7.2%) samples tested by Rose Bengal Plate Test (RBPT) and in 41 (6.7%) samples tested by Milk Ring Test (MRT). The isolation rate was 29.6% (16 out of 54 examined samples). Brucella organism was isolated from three aborted fetuses, one tissue sample of slaughtered serologically positive ewe and 12 milk samples. The Abortus Melitensis Ovis Suis-PCR (AMOS-PCR) confirmed all Brucella strains as B. melitensis. More than three successive negative serological tests are required to declare that the infected herd is free from brucellosis. In conclusion, no single serological test could conclusively diagnose brucellosis in endemic areas. Confirmation of results with molecular diagnosis or culture is indispensable in diagnosis. B. melitensis was the prevalent serotype among sheep in Dakahlia governorate


2013 ◽  
Vol 26 (2) ◽  
pp. 68-71 ◽  
Author(s):  
Khurshida Tahmin ◽  
Shamsun Nahar Begum

This Randomised controlled clinical trial was undertaken to determine the suitable time of catheter removal after urogenital prolapse surgery in order to reduce the catheter related UTI and its long term sequel. Method: Total 80 patients undergoing vaginal hysterectomy with pelvic floor repair were enrolled and randomised into two equal groups by lottery method. Trans-urethral catheter was removed on 2nd & 5th post operative day in group I (n=40) and in group II (n=40) respectively. Post   voidal residual urine volume before operation and after removal of catheter was measured in   all patients by ultrasound scanning. Re-catheterisation was done for three more days if residual volume > 200ml after removal of catheter. Urine samples were taken before removal of catheter and send for routine microscopic examination and culture & sensitivity test to detect UTI.Results: Removal of catheter on 2nd post-operative day lead to significant reduction of rate of UTI. The rate of UTI following removal of catheter was 7.5% versus 42.5% in group I and in group II (p<0.001). But need for recatheterisation was significantly higher in group I (15%) than in group II (2.5%). The mean duration of catheterisation was 47.63 (±0.628) and 119.35 (±0.864) hours in group I and group II respectively. Majority of the patients in group I did not  require prolongation of catheterisation. Mean duration of hospital stay after operation was 5 days in group I and 7.95 days in group II (p<0.001). Conclusion: Short period catheterisation is associated with lower rate of UTI and shorter hospital stay. Disadvantages of prolonged catheterisation outweigh the advantages. Therefore removal of the catheter on the 2nd post-operative day after genital prolapse surgery is preferable than 5 days catheterization DOI: http://dx.doi.org/10.3329/bjog.v26i2.13782 Bangladesh J Obstet Gynaecol, 2011; Vol. 26(2) : 68-71


1988 ◽  
Vol 83 (2) ◽  
pp. 219-225 ◽  
Author(s):  
Rita Maria R. Nogueira ◽  
Hermann G. Schatzmayr ◽  
Marize P. Miagostovich ◽  
Maria de Fátima D. B. Farias ◽  
José da Costa Farias Filho

A dengue outbreak started in March, 1986 in Rio de Janeiro and spread very rapidly to other parts of the country. The great majority of cases presented classical dengue fever but there was one fatal case, confirmed by virus isolation. Dengue type 1 strains were isolated from patients and vectors (Aedes aegypti) in the area by cultivation in A. albopictus C6/36 cell line. The cytopathic effect (CPE) was studied by electron microscopy. An IgM capture test (MAC-ELISA) was applied with clear and reproducible results for diagnosis and evaluation of virus circulation; IgM antibodies appeared soon after start of clinical disease, and persisted for about 90 days in most patients. The test was type-specific in about 50% of the patients but high levels of heterologous response for type 3 were observed. An overall isolation rate of 46,8% (813 virus strains out of 1734 specimens) was recorded. The IgM test increased the number of confirmed cases to 58,2% (1479 out of 2451 suspected cases). The importance of laboratory diagnosis in all regions where the vectors are present is emphasized.


Sign in / Sign up

Export Citation Format

Share Document