Three-dimensional, polarization-sensitive, spectroscopic photon localization microscopy for parallel single-molecules imaging and tracking (Conference Presentation)

2018 ◽  
Author(s):  
Biqin Dong ◽  
Brian T. Soetikno ◽  
Xiangfan Chen ◽  
Vadim Backman ◽  
Cheng Sun ◽  
...  
Keyword(s):  
Single Molecules ◽  
Three Dimensional ◽  
Localization Microscopy ◽  
Photon Localization

Parallel Three-Dimensional Tracking of Quantum Rods Using Polarization-Sensitive Spectroscopic Photon Localization Microscopy

ACS Photonics ◽  
2017 ◽  
Vol 4 (7) ◽  
pp. 1747-1752 ◽  
Author(s):  
Biqin Dong ◽  
Brian T. Soetikno ◽  
Xiangfan Chen ◽  
Vadim Backman ◽  
Cheng Sun ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Localization Microscopy ◽  
Quantum Rods ◽  
Photon Localization

scholarly journals Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Alan M. Szalai ◽  
Bruno Siarry ◽  
Jerónimo Lukin ◽  
David J. Williamson ◽  
Nicolás Unsain ◽  
...  
Keyword(s):  
Single Molecule ◽  
Total Internal Reflection ◽  
Single Molecules ◽  
Fluorescence Microscope ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Axial Position ◽  
Localization Microscopy ◽  
Localization Precision ◽  
Single Molecule Localization Microscopy

AbstractSingle-molecule localization microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision.

scholarly journals Spatial filter and its application in three-dimensional single molecule localization microscopy

2020 ◽  
Vol 8 (2) ◽  
pp. 025008
Author(s):  
Xiaoming Fan ◽  
Johnny Hendriks ◽  
Maddalena Comini ◽  
Alexandros Katranidis ◽  
Georg Büldt ◽  
...  
Keyword(s):  
Single Molecule ◽  
Three Dimensional ◽  
Spatial Filter ◽  
Localization Microscopy ◽  
Single Molecule Localization Microscopy

Two-Photon Microscopy of Single Molecules

2000 ◽  
Vol 6 (S2) ◽  
pp. 802-803
Author(s):  
J. T. Fourkas ◽  
M. J. R. Previte ◽  
R. A. Farrer ◽  
C. Olson ◽  
L. A. Peyser
Keyword(s):  
Near Field ◽  
Single Molecules ◽  
Three Dimensional ◽  
Excitation Beam ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Higher Power ◽  
Excitation Techniques ◽  
Field Excitation ◽  
Heterogeneous Ensemble

The ability to observe the fluorescence arising from single molecules has revolutionized our ability to study the structure and dynamics of materials on a microscopic level and to probe the properties of individual members of a heterogeneous ensemble. A variety of near-field and far-field excitation techniques have been employed to study single molecules. Multiphoton excitation (MPE) techniques have a number of advantages that make them particularly attractive for singlemolecule detection. First, because the excitation and fluorescence wavelengths are significantly different from one another, Rayleigh and Raman scattering can easily be filtered out, leading to a low number of background counts. Second, because the probability for MPE depends on the excitation intensity to the second or higher power, the excitation is localized to the point in space where the excitation beam is most tightly focussed, thus providing three-dimensional resolution.

scholarly journals Three-dimensional localization microscopy in live flowing cells

2020 ◽  
Vol 15 (6) ◽  
pp. 500-506 ◽  
Author(s):  
Lucien E. Weiss ◽  
Yael Shalev Ezra ◽  
Sarah Goldberg ◽  
Boris Ferdman ◽  
Omer Adir ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Localization Microscopy

Three-Dimensional Orientations of Polymer-Bound Single Molecules

1999 ◽  
Vol 103 (16) ◽  
pp. 3053-3056 ◽  
Author(s):  
Andrew P. Bartko ◽  
Robert M. Dickson
Keyword(s):  
Single Molecules ◽  
Three Dimensional

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels

Science ◽  
1996 ◽  
Vol 274 (5289) ◽  
pp. 966-968 ◽  
Author(s):  
Robert M. Dickson ◽  
D. J. Norris ◽  
Yih-Ling Tzeng ◽  
W. E. Moerner
Keyword(s):  
Single Molecules ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Poly Acrylamide
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