Red blood cells interaction mediated by dextran macromolecules: in vitro study using diffuse light scattering technique and optical tweezers

2019 ◽  
Author(s):  
Alexey Semenov ◽  
Andrei Lugovtsov ◽  
Sehyun Shin ◽  
Gregory Barshtein ◽  
Alexander V. Priezzhev
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Diffuse Light ◽  
Scattering Technique ◽  
Light Scattering Technique

scholarly journals Necrotic and apoptotic volume changes of red blood cells investigated by low-angle light scattering technique

2007 ◽  
Vol 21 (2) ◽  
pp. 105-120 ◽  
Author(s):  
Igor V. Mindukshev ◽  
Vladimir V. Krivoshlyk ◽  
Elena E. Ermolaeva ◽  
Irina A. Dobrylko ◽  
Evgeniy V. Senchenkov ◽  
...  
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Platelets ◽  
Calcium Ionophore ◽  
Scattering Technique ◽  
Rbc Aggregation ◽  
Ammonia Medium ◽  
Very High ◽  
Light Scattering Technique

A low-angle light scattering technique, which has been applied previously to studies of blood platelets and Ehrlich ascite tumor cells, revealed differences in the dynamics of necrotic and apoptotic red blood cell death. Under hypotonic loading or in ammonia medium, red blood cells (RBC) swelled to a critical size (diameter approximately 13μm) prior to hemolysis (necrosis). Under acidic loading, hemolysis occurred with less pronounced swelling of cells (diameter approximately 10μm). Apoptosis induced by a calcium ionophore resulted in initial formation of echinocytes, followed by development of rounded red blood cells with uneven membrane, capable of agglomeration. In such a way, RBC aggregation can precede the final stages of the RBC apoptosis when small cellular fragments are generated. On the basis of erythrograms of the cells hemolysing in ammonia medium, the echinocytic (preapoptotic) and stomatocytic (prenecrotic) RBC were discerned due to the very high resistance of apoptotic RBC to osmotic (ammonia) loading.

scholarly journals An In Vitro Study of the Effect of Cytotoxic Triorganotin Dimethylaminophenylazobenzoate Complexes on Red Blood Cells

2018 ◽  
Vol 251 (5-6) ◽  
pp. 735-745 ◽  
Author(s):  
Hanna Pruchnik ◽  
Aleksandra Włoch ◽  
Dorota Bonarska-Kujawa ◽  
Halina Kleszczyńska
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

In vitro study on the partitioning of red blood cells using a microchannel network

2022 ◽  
Vol 140 ◽  
pp. 104281
Author(s):  
Toru Hyakutake ◽  
Hiroki Abe ◽  
Yohei Miyoshi ◽  
Manabu Yasui ◽  
Rina Suzuki ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Evaluation of chromium in red blood cells as an indicator of exposure to hexavalent chromium: An in vitro study

2016 ◽  
Vol 255 ◽  
pp. 63-70 ◽  
Author(s):  
Jérôme Devoy ◽  
Antoine Géhin ◽  
Samuel Müller ◽  
Mathieu Melczer ◽  
Aurélie Remy ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Hexavalent Chromium ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

In vitro study of the specific interaction between poly(2-dimethylamino ethylmethacrylate) based polymers with platelets and red blood cells

2015 ◽  
Vol 492 (1-2) ◽  
pp. 55-64 ◽  
Author(s):  
Luca Flebus ◽  
François Lombart ◽  
Lucía Martinez-Jothar ◽  
Chantal Sevrin ◽  
Céline Delierneux ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Specific Interaction ◽  
In Vitro Study ◽  
Vitro Study

Influence of plasma preparations and donor red blood cells on the antioxidant capacity of blood from newborn babies: an in vitro study

1996 ◽  
Vol 85 (2) ◽  
pp. 220-224 ◽  
Author(s):  
RMW Moison ◽  
EJHA Hoof ◽  
PC Clahsen ◽  
D Zoeren-Grobben ◽  
HM Berger
Keyword(s):  
Antioxidant Capacity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Assessment of Fibrinogen Macromolecules Interaction with Red Blood Cells Membrane by Means of Laser Aggregometry, Flow Cytometry, and Optical Tweezers Combined with Microfluidics

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1448
Author(s):  
Alexey N. Semenov ◽  
Andrei E. Lugovtsov ◽  
Evgeny A. Shirshin ◽  
Boris P. Yakimov ◽  
Petr B. Ermolinskiy ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Significant Risk ◽  
In Vitro Study ◽  
Significant Risk Factor ◽  
Elevated Concentration ◽  
Rbc Membrane ◽  
Rbc Aggregation

An elevated concentration of fibrinogen in blood is a significant risk factor during many pathological diseases, as it leads to an increase in red blood cells (RBC) aggregation, resulting in hemorheological disorders. Despite the biomedical importance, the mechanisms of fibrinogen-induced RBC aggregation are still debatable. One of the discussed models is the non-specific adsorption of fibrinogen macromolecules onto the RBC membrane, leading to the cells bridging in aggregates. However, recent works point to the specific character of the interaction between fibrinogen and the RBC membrane. Fibrinogen is the major physiological ligand of glycoproteins receptors IIbIIIa (GPIIbIIIa or αIIββ3 or CD41/CD61). Inhibitors of GPIIbIIIa are widely used in clinics for the treatment of various cardiovascular diseases as antiplatelets agents preventing the platelets’ aggregation. However, the effects of GPIIbIIIa inhibition on RBC aggregation are not sufficiently well studied. The objective of the present work was the complex multimodal in vitro study of the interaction between fibrinogen and the RBC membrane, revealing the role of GPIIbIIIa in the specificity of binding of fibrinogen by the RBC membrane and its involvement in the cells’ aggregation process. We demonstrate that GPIIbIIIa inhibition leads to a significant decrease in the adsorption of fibrinogen macromolecules onto the membrane, resulting in the reduction of RBC aggregation. We show that the mechanisms underlying these effects are governed by a decrease in the bridging components of RBC aggregation forces.

Eryptosis-inducing activity of bisphenol A and its analogs in human red blood cells (in vitro study)

2016 ◽  
Vol 307 ◽  
pp. 328-335 ◽  
Author(s):  
Aneta Maćczak ◽  
Monika Cyrkler ◽  
Bożena Bukowska ◽  
Jaromir Michałowicz
Keyword(s):  
Bisphenol A ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Red Blood Cells
Sign in / Sign up

Export Citation Format

Share Document