Consumption of supplementary inulin modulates milk microbiota and metabolites in dairy cows with subclinical mastitis

2021 ◽  
Author(s):  
Yue Wang ◽  
Xuemei Nan ◽  
Yiguang Zhao ◽  
Linshu Jiang ◽  
Hui Wang ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Dairy Cows ◽  
Basal Diet ◽  
Subclinical Mastitis ◽  
Leukotriene B4 ◽  
Control Group ◽  
Gastrointestinal Microbiota ◽  
Main Determinant ◽  
Metabolic Activities ◽  
Prostaglandin A1

Milk microbiota and mediated metabolites directly affect the health of the udder in dairy cows. Inulin, a dietary prebiotic, can modulate the profile of gastrointestinal microbiota. However, whether the inulin intake affects the milk microbial population and metabolites remains unknown. In this study, forty subclinical mastitis (SCM) cows were randomly divided into 5 groups. Five inulin addition doses, 0, 100, 200, 300 and 400 g/d per cow, based on the same basal diet were supplemented, respectively. The experiments lasted for 8 weeks. The results showed lower relative abundance of mastitic-causing and pro-inflammation microbes in milk (i.e., Escherichia -Shigella , Pseudomonas , Rhodococcus and Burkholderia-Caballeronia-Paraburkholderia , etc.), and higher probiotics and commensal bacteria, such as, Lactobacillus , Bifidobacterium , etc. in the cows fed 300 g/d inulin, compared with the control group. Meanwhile, the levels of arachidonic acid pro-inflammatory mediators (leukotriene E3, 20-carboxy-leukotriene B4 and 12-Oxo-c-LTB3) and phospholipid metabolites were reduced, and the levels of compounds with antibacterial and anti-inflammatory potential (prostaglandin A1 and 8-iso-15-Keto-PGE 2 , etc.), and participating energy metabolism (citric acid and L-Carnitine, etc.) were elevated. These data suggested that inulin intake might modulate the microflora and metabolites level in extra-intestinal tissue, such as mammary gland, which provided an alternative for the regulation and mitigation for SCM. IMPORTANCE The profile of microbial community and metabolic activity in milk are main determinant of udder health status and milk quality. Recent studies have demonstrated that diet could directly modulate mammary gland microbiome. Inulin is a probiotic dietary fiber, which can improve the microbiota population in gastrointestinal tract. However, whether inulin intake can further regulate the profile of microbiota and metabolic activities in milk remains unclear. In subclinical mastitic cows, we found that inulin supplementation could reduce the abundance of Escherichia -Shigella , Pseudomonas , Rhodococcus , Burkholderia-Caballeronia-Paraburkholderia and the levels of (±)12, 13 - DiHOME, leukotriene E3 and 20-Carboxy-Leukotriene B4 etc., while, elevated the abundance of Lactobacillus , Bifidobacterium , and Muribaculaceae as well as the levels of prostaglandin A1 (PGA1), 8-iso-15-keto-PGE2 and benzoic acid etc., in milk. These data suggest that inulin intake affects the profile of microorganisms and metabolites in milk, which provides an alternative for the regulation of mastitis.

scholarly journals Modulation of Gut Microbiota and Oxidative Status by β-Carotene in Late Pregnant Sows

2020 ◽  
Vol 7 ◽  
Author(s):  
Xupeng Yuan ◽  
Jiahao Yan ◽  
Ruizhi Hu ◽  
Yanli Li ◽  
Ying Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Fecal Microbiota ◽  
Control Group ◽  
Metabolic Activities ◽  
The Impact ◽  
Positive Effect ◽  
Β Carotene

Recent evidences suggest that gut microbiota plays an important role in regulating physiological and metabolic activities of pregnant sows, and β-carotene has a potentially positive effect on reproduction, but the impact of β-carotene on gut microbiota in pregnant sows remains unknown. This study aimed to explore the effect and mechanisms of β-carotene on the reproductive performance of sows from the aspect of gut microbiota. A total of 48 hybrid pregnant sows (Landrace × Yorkshire) with similar parity were randomly allocated into three groups (n = 16) and fed with a basal diet or a diet containing 30 or 90 mg/kg of β-carotene from day 90 of gestation until parturition. Dietary supplementation of 30 or 90 mg/kg β-carotene increased the number of live birth to 11.82 ± 1.54 and 12.29 ± 2.09, respectively, while the control group was 11.00 ± 1.41 (P = 0.201). Moreover, β-carotene increased significantly the serum nitric oxide (NO) level and glutathione peroxidase (GSH-Px) activity (P < 0.05). Characterization of fecal microbiota revealed that 90 mg/kg β-carotene increased the diversity of the gut flora (P < 0.05). In particular, β-carotene decreased the relative abundance of Firmicutes including Lachnospiraceae AC2044 group, Lachnospiraceae NK4B4 group and Ruminococcaceae UCG-008, but enriched Proteobacteria including Bilophila and Sutterella, and Actinobacteria including Corynebacterium and Corynebacterium 1 which are related to NO synthesis. These data demonstrated that dietary supplementation of β-carotene may increase antioxidant enzyme activity and NO, an important vasodilator to promote the neonatal blood circulation, through regulating gut microbiota in sows.

scholarly journals Nano-selenium Supplementation Increases Selenoprotein (Sel) Gene Expression Profiles and Milk Selenium Concentration in Lactating Dairy Cows

2020 ◽  
Vol 199 (1) ◽  
pp. 113-119
Author(s):  
Liqiang Han ◽  
Kun Pang ◽  
Tong Fu ◽  
Clive J. C. Phillips ◽  
Tengyun Gao
Keyword(s):  
Gene Expression ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Sodium Selenite ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Selenium Concentration ◽  
Basal Diet ◽  
Control Group ◽  
Lactation Performance

AbstractSupplementation with selenium is common for dairy cows, but the importance of selenium source is not clear. This study aimed to compare nano-selenium (Nano-Se) and sodium selenite supplements for dairy cows on lactation performance, milk Se levels and selenoprotein (Sel) gene expression. Twelve multiparous Holstein cows were randomly divided into two groups: a control group fed a basal diet plus 0.30 mg Se/kg of DM as sodium selenite or Nano-Se for 30 days. Dry matter intake, milk yield and composition were not affected by dietary Se source (P > 0.05); however, the milk total Se levels and milk glutathione peroxidase (GSH-Px) activities were higher with Nano-Se supplementation than sodium selenite (P < 0.05). At the end of the experiment, Nano-Se supplementation significantly increased plasma Se levels and GSH-Px activity, compared with the sodium selenite supplement. The mRNA expression levels of glutathione peroxidase 1, 2 and 4; thioredoxin reductase 2 and 3; and selenoproteins W, T, K and F were markedly upregulated (P < 0.05) in the mammary gland of the Nano-Se group. Thus, the source of selenium plays an important role in the antioxidant status and in particular the Sel gene expression in the mammary glands of dairy cows, both being stimulated by nano sources.

Effect of parenteral administration of Selenium and vitamin E on health status of mammary gland and on selected antioxidant indexes in blood of dairy cows

2014 ◽  
Vol 17 (2) ◽  
pp. 217-223 ◽  
Author(s):  
F. Zigo ◽  
Z. Farkasóvá ◽  
J. Eleko ◽  
M. Lapin ◽  
M. Chripková ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Health Status ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Clinical Mastitis ◽  
Parenteral Administration ◽  
Control Group ◽  
Blood Samples ◽  
Experimental Group

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

Milk production responses to turnips fed to dairy cows in mid lactation

1998 ◽  
Vol 38 (2) ◽  
pp. 117 ◽  
Author(s):  
P. J. Moate ◽  
D. E. Dalley ◽  
K. Martin ◽  
C. Grainger
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Basal Diet ◽  
Milk Composition ◽  
Control Group ◽  
Physical Form ◽  
Yield Responses ◽  
Dietary Treatments

Summary. Recently, many dairy farmers in Victoria have grown turnips as a summer fodder crop for lactating dairy cows. This paper reports on a 1 month experiment in which cows were individually fed indoors, and milk yield responses to feeding combinations of turnips and barley were measured. Forty cows in mid lactation were evenly allocated to 5 treatment groups. The control group were offered a basal diet of pasture hay and pasture silage which is similar to that available on many Victorian dairy farms during summer. Cows in the other 4 groups were offered the basal diet and various combinations of turnips and/or barley. Marginal milk responses from barley, turnips and a 50 : 50 mixture of turnips and barley were 0.62, 0.49 and 0.59 L/kg dry matter of supplement eaten. Milk composition of all groups was similar. Despite diets differing greatly in the proportions and physical form of dietary constituents, the mean milk production of each group was accurately predicted by their mean dry matter intake. Measurement of plasma minerals (calcium, magnesium, sodium, potassium and phosphorus) and rumen fluid pH showed that dietary treatments caused some significant (P<0.05) differences but all values were within the normal range. In this experiment, the milk production responses from barley, turnips and combinations of barley and turnips were similar. Since the cost of turnips is generally about half that of barley, it is concluded that turnips are an economic alternative to barley as a feed supplement for summer milk production.

scholarly journals Mammary gland health of Santa Inês ewes at the drying and puerperium and evaluation of a dry-off terapy with gentamicin

2018 ◽  
Vol 38 (12) ◽  
pp. 2194-2200
Author(s):  
Priscilla F.V. Pereira ◽  
Ana P. Reway ◽  
Aline Félix ◽  
Edsel A. Beutemmüller ◽  
Lucienne G. Pretto-Giordano ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Control Group ◽  
Coagulase Negative Staphylococcus ◽  
California Mastitis Test ◽  
Important Health ◽  
Santa Inês ◽  
Staphylococcus Spp

ABSTRACT: Mastitis represents an important health problem for Santa Inês breed, causing losses to the producer, due to loss of ewes or the decrease in weight gain of lambs. The aim of this work was to assess the health of the mammary gland of Santa Inês ewes at the drying and puerperium and to investigate the efficacy of a dry-off therapy with gentamicin. In this study, 64 ewes were divided in a control group (GC) and treatment group (GT), and the health of the mammary gland was assessed at the drying and puerperium. The GT ewes received 250mg of gentamicin (Gentocin® DryCow/Schering-Plough, product indicated for use in dairy cows) in each mammary half. For diagnosis, clinical examination, California Mastitis Test, somatic cell count and milk culture was performed. In the GC, of the 45 (70.3%) healthy mammary halves at the drying, 12 developed subclinical mastitis and nine clinical mastitis at the puerperium. In the GT, among 51 (79.7%) healthy mammary halves at the drying, six developed subclinical mastitis and 11 clinical mastitis at the puerperium. No association was observed between treatment and the occurrence of mastitis at puerperium. Of the 19 (29.7%) mammary halves of the GC that presented subclinical mastitis at the drying, three remained with subclinical mastitis and five developed clinical mastitis at the puerperium. In the GT, of the 13 (20.3%) mammary halves that had subclinical mastitis at the drying, four remained with subclinical mastitis and four developed clinical mastitis. No association was observed between treatment and cure or persistence of mastitis at the puerperium. The main microorganisms isolated, at the drying and puerperium, from animals with subclinical or clinical mastitis were Staphylococcus spp., predominantly coagulase negative Staphylococcus (CSN). At the puerperium, 29 cases of clinical mastitis occurred, 19 with isolation, where 10 were CNS and six S. aureus. Mannheimia haemolytica was isolated in one case of subclinical mastitis and other of clinical mastitis. News protocols and different ways of handling at drying and at puerperium must be investigated.

scholarly journals Influence of rumen-protected choline on blood red-ox potential and biochemical biomarkers of dairy cows during the transition period

2017 ◽  
Vol 73 (8) ◽  
pp. 483-487
Author(s):  
Mustafa Salman ◽  
Gülay Çiftçi ◽  
Alper Çiftçi
Keyword(s):  
Dairy Cows ◽  
Transition Period ◽  
Basal Diet ◽  
Oxidative Status ◽  
The Other ◽  
Stress Index ◽  
Control Group ◽  
Oxidative Stress Index ◽  
Treatment Groups ◽  
The Third

The aim of this study was to determine the serum oxidative status, antioxidant capacity, and oxidative stress index, as well as aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatinine profiles, of dairy cows fed rumen-protected choline during the transition period. A total of thirty dairy cows allotted to one control and two experimental groups (10 animals per group) were fattened from 3 weeks prior to the expected calving date until 3 weeks postpartum. The control group was fed a basal diet, and treatment groups 1 and 2, were fed a basal diet containing 60 g/head/day and 120 g/head/day or rumen-protected choline, respectively. Serum total antioxidant status (TAS) levels for the control group and treatment groups 1 and 2 at the time of parturition were 0.56±0.029ab, 0.45±0.028b, and 0.59±0.036a, respectively. The differences between the groups were statistically significant (P<0.05). Rumen-protected choline supplementation did not have a significant effect on TAS levels in the third week before parturition and in the first and third weeks of lactation. There were no significant differences (P>0.05) between the groups in terms of total oxidative status (TOS), oxidative stress index (OSI) or creatinine levels. Serum ALT levels were significantly different (P<0.05) between the groups at parturition and in the first week of lactation, but not significant (P>0.05) for the other periods. In addition, the serum AST level was significantly different (P<0.05) between the groups in the first and third weeks of lactation, but not significantly different (P>0.05) for the other periods. After removing the effect of groups, time-dependent changes were significant (P<0.001) for all parameters. The highest values for TAS, TOS and OSI were obtained in the third week before parturition. The values for these parameters decreased during parturition and after the postnatal period. The lowest values for serum ALT and creatinine occurred at parturition. Rumen-protected choline supplementation did not have a significant effect on serum TAS and TOS levels during the transition period. There is a need for further studies of animals with known metabolic profiles, malnourished animals, and those with high milk yields or metabolic disorders.

scholarly journals Functional state of the udder of cows after the treatment of the udder nipples with hygiene products during milking

2021 ◽  
Vol 36 ◽  
pp. 06035
Author(s):  
A.V. Filatova ◽  
Yu.V. Bibaeva ◽  
S.V. Kozlov ◽  
M.V. Nistratova ◽  
V.S. Avdeenko
Keyword(s):  
Mammary Gland ◽  
Latent Period ◽  
Olive Oil ◽  
Dairy Cows ◽  
Milk Yield ◽  
Functional State ◽  
Subclinical Mastitis ◽  
Control Groups ◽  
Controlled Treatment ◽  
Before And After

During the experiments, it was found that the latent period of the breast-feeding reflex is 22.8% less, the milking time is 10.8% less. The average milking intensity increases by 22.7%, intravenous pressure - by 19.4%, with an increase in single milk yield by 15.3%. The concentration of Staph. aigees, Strep. agalactiae, was lower when the nipples were immersed in a solution of hygiene products "Chlorhexidine bigluconate-aloe" and "Teasfoam Supercow-olive oil" before and after milking, compared with untreated nipples. Controlled treatment of the udder nipples and mammary gland with the hygienic product "HGB aloe" before and after cow milking with high milk yields in 2.4% of animals prevents hyperkeratosis of the udder nipples and in 12.1% subclinical mastitis, against 5.4% heperkeratosis and 26.5% subclinical mastitis of control groups of dairy cows. Using hygienic products, an increase in milk yield in cows is provided by 18.45...22.0%, and the functioning of the udder by 16.45...26.34 %. To improve the functioning of the udder during lactation in cows, hygiene products "Chlorhexidine bigluconate-aloe" or "Teasfoam Supercow–olive oil" should be used to treat the udder nipples before and after each milking.

scholarly journals Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows

2020 ◽  
Author(s):  
Kun Hou ◽  
Jinjin Tong ◽  
Hua Zhang ◽  
Shan Gao ◽  
Yuqin Guo ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Dairy Cows ◽  
Dairy Cow ◽  
Milk Fat ◽  
Enrichment Analysis ◽  
Control Group ◽  
Milk Samples ◽  
Metabolite Profiles ◽  
Metabolomics Analysis ◽  
Potential Biomarkers

Abstract This study aimed to explore the effects of artemisinin (ART) on the milk microbiome and metabolites of dairy cow. A total of 12 mid-lactation Holstein dairy cows with similar parity, days in milk were randomly divided into 2 groups receiving either a total mixed ration (TMR) as the control group or this TMR and 120 g/d/head ART as the ART group. The milk samples were collected weekly to determine the contents, and end-of-trial (wk 8) milk samples were used to identify microbial species and metabolite profiles by 16S rRNA and LC-MS analyses, respectively. We observed that the milk fat content significantly increased by ART treantment (P < 0.05). The bacterial community richness was significantly lower in the ART group (P < 0.05), while the diversity showed no difference (P > 0.05). Compared with its abundance in the CON group, Firmicutes was significantly decreased, whereas Proteobacteria was significantly increased. Furthermore, in the ART group, the relative abundances of the genera Aerococcus, Staphylococcus, Corynebacterium_1 and Facklamia were significantly lower (P < 0.01). Metabolomics analysis revealed that ART significantly increasing the concentrations of glycerophospholipids, glycerolipids and flavonoids compared with those in the CON group. An enrichment analysis of the different metabolites showed that ART mainly affected glycerophospholipid metabolism and the pantothenate and CoA biosynthesis pathways. These findings revealed that ART supplementation could affect the milk microbiota and metabolites, that glycerophospholipids and glycerolipids could be potential biomarkers in the milk response to ART feed in dairy cows, and that ART changes substances in milk by maintaining lipid metabolism in the mammary gland.

scholarly journals Plasma FGF21 Is Elevated by the Intense Lipid Mobilization of Lactation

Endocrinology ◽  
2011 ◽  
Vol 152 (12) ◽  
pp. 4652-4661 ◽  
Author(s):  
Katie M. Schoenberg ◽  
Sarah L. Giesy ◽  
Kevin J. Harvatine ◽  
Matthew R. Waldron ◽  
Christine Cheng ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Dairy Cows ◽  
Late Pregnancy ◽  
Energy Deficit ◽  
Fibroblast Growth Factor 21 ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Metabolic Activities ◽  
Elevated Lipid ◽  
Mammary Gland Expression

In many mammals, lactation success depends on substantial use of lipid reserves and requires integrated metabolic activities between white adipose tissue (WAT) and liver. Mechanisms responsible for this integration in lactation are poorly understood, but data collected in other conditions of elevated lipid use suggest a role for fibroblast growth factor-21 (FGF21). To address this possibility in the context of lactation, we studied high-yielding dairy cows during the transition from late pregnancy (LP) to early lactation (EL). Plasma FGF21 was nearly undetectable in LP, peaked on the day of parturition, and then stabilized at lower, chronically elevated concentrations during the energy deficit of EL. Plasma FGF21 was similarly increased in the absence of parturition when an energy-deficit state was induced by feed restricting late-lactating dairy cows, implicating energy insufficiency as a cause of chronically elevated FGF21 in EL. Gene expression studies showed that liver was a major source of plasma FGF21 in EL with little or no contribution by WAT, skeletal muscle, and mammary gland. Meaningful expression of the FGF21 coreceptor β-Klotho was restricted to liver and WAT in a survey of 15 tissues that included the mammary gland. Expression of β-Klotho and its subset of interacting FGF receptors was modestly affected by the transition from LP to EL in liver but not in WAT. Overall, these data suggest a model whereby liver-derived FGF21 regulates the use of lipid reserves during lactation via focal actions on liver and WAT.

scholarly journals Evaluation of the Prevalence of Subclinical Mastitis in Dairy Cattle in the Soummam Valley (Bejaia, Algeria)

2019 ◽  
Vol 76 (2) ◽  
pp. 143
Author(s):  
Asma Ait KAKI ◽  
Salem DJEBALA ◽  
Muhammad Bilal LATIF ◽  
Nassim MOULA
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Dairy Cattle ◽  
Dairy Cows ◽  
Farming System ◽  
Subclinical Mastitis ◽  
Bacterial Strains ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Bacterium Species

The present study was carried out to investigate the prevalence of subclinical mastitis (SCM) in milking cows and the effect of farming system on the prevalence of SCM”, as well as the identification of bacterial strains causing the mammary gland infection. A total of 100 dairy cows were randomly selected from 32 farms of Bejaia district (Algeria), precisely the Soummam region: from Tazmalt to El Kseur. The SCM was screened by California Mastitis Test (CMT). Milk samples were collected aseptically from (CMT) positive cows and dispatched to laboratory for further microbiological tests. Results showed that the prevalence of SCM was 26%. Moreover, no significant differences in SCM prevalence were observed between intensive and semi intensive systems (P>0.05). The Staphylococcus aureus was found to be the most frequent bacterium species associated with SCM in the cows studied.

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