scholarly journals Integrated Regulation of Acetoin Fermentation by Quorum Sensing and pH in Serratia plymuthica RVH1

2011 ◽  
Vol 77 (13) ◽  
pp. 4704-4704 ◽  
Author(s):  
Pieter Moons ◽  
Rob Van Houdt ◽  
Bram Vivijs ◽  
Chris W. Michiels ◽  
Abram Aertsen
Keyword(s):  
Quorum Sensing ◽  
Serratia Plymuthica ◽  
Integrated Regulation

Quorum sensing and butanediol fermentation affect colonization and spoilage of carrot slices by Serratia plymuthica

2009 ◽  
Vol 134 (1-2) ◽  
pp. 63-69 ◽  
Author(s):  
Eva Wevers ◽  
Pieter Moons ◽  
Rob Van Houdt ◽  
Ine Lurquin ◽  
Abram Aertsen ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Serratia Plymuthica

scholarly journals Role of Quorum Sensing and Antimicrobial Component Production by Serratia plymuthica in Formation of Biofilms, Including Mixed Biofilms with Escherichia coli

2006 ◽  
Vol 72 (11) ◽  
pp. 7294-7300 ◽  
Author(s):  
Pieter Moons ◽  
Rob Van Houdt ◽  
Abram Aertsen ◽  
Kristof Vanoirbeek ◽  
Yves Engelborghs ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Homoserine Lactone ◽  
Broth Culture ◽  
Confocal Laser ◽  
Wild Type ◽  
Serratia Plymuthica ◽  
E Coli

ABSTRACT We have previously characterized the N-acyl-l-homoserine lactone-based quorum-sensing system of the biofilm isolate Serratia plymuthica RVH1. Here we investigated the role of quorum sensing and of quorum-sensing-dependent production of an antimicrobial compound (AC) on biofilm formation by RVH1 and on the cocultivation of RVH1 and Escherichia coli in planktonic cultures or in biofilms. Biofilm formation of S. plymuthica was not affected by the knockout of splI or splR, the S. plymuthica homologs of the luxI or luxR quorum-sensing gene, respectively, or by the knockout of AC production. E. coli grew well in mixed broth culture with RVH1 until the latter reached 8.5 to 9.5 log CFU/ml, after which the E. coli colony counts steeply declined. In comparison, only a very small decline occurred in cocultures with the S. plymuthica AC-deficient and splI mutants. Complementation with exogenous N-hexanoyl-l-homoserine lactone rescued the wild-type phenotype of the splI mutant. The splR knockout mutant also induced a steep decline of E. coli, consistent with its proposed function as a repressor of quorum-sensing-regulated genes. The numbers of E. coli in 3-day-old mixed biofilms followed a similar pattern, being higher with S. plymuthica deficient in SplI or AC production than with wild-type S. plymuthica, the splR mutant, or the splI mutant in the presence of N-hexanoyl-l-homoserine lactone. Confocal laser scanning microscopic analysis of mixed biofilms established with strains producing different fluorescent proteins showed that E. coli microcolonies were less developed in the presence of RVH1 than in the presence of the AC-deficient mutant.

scholarly journals Quorum-sensing effects in the antagonistic rhizosphere bacterium Serratia plymuthica HRO-C48

2009 ◽  
Vol 67 (3) ◽  
pp. 468-478 ◽  
Author(s):  
Henry Müller ◽  
Christian Westendorf ◽  
Erich Leitner ◽  
Leonid Chernin ◽  
Kathrin Riedel ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Serratia Plymuthica ◽  
Rhizosphere Bacterium

scholarly journals Integrated Regulation of Acetoin Fermentation by Quorum Sensing and pH in Serratia plymuthica RVH1

2011 ◽  
Vol 77 (10) ◽  
pp. 3422-3427 ◽  
Author(s):  
Pieter Moons ◽  
Rob Van Houdt ◽  
Bram Vivijs ◽  
Chris M. Michiels ◽  
Abram Aertsen
Keyword(s):  
Quorum Sensing ◽  
Bacterial Species ◽  
Homoserine Lactone ◽  
Positive Control ◽  
Negative Control ◽  
Serratia Plymuthica ◽  
Content Type ◽  
Environmental Signals ◽  
Mixed Acid ◽  
External Stimuli

ABSTRACTDuring fermentation of sugars, a number of bacterial species are able to switch from mixed acid production to acetoin and 2,3-butanediol production in order to avoid lethal acidification of their environment, although the regulation of this switch is only poorly understood. In this study, we report the identification of thebudABstructural operon, involved in acetoin production inSerratia plymuthicaRVH1, and its activation by a LysR-type regulator encoded bybudR, immediately upstream of this operon. In addition, the regulation ofbudRtranscription was elucidated and found to be subject to negative control by BudR itself and to positive control by external stimuli such asN-(3-oxohexanoyl)-l-homoserine lactone (OHHL) quorum sensing signaling molecules and acetate. Interestingly, however, we observed that induction ofbudRtranscription by OHHL or acetate did not require BudR, indicating the involvement of additional regulatory factors in relaying these environmental signals to thebudRpromoter.

scholarly journals N-Acyl-l-Homoserine Lactone Quorum Sensing Controls Butanediol Fermentation in Serratia plymuthica RVH1 and Serratia marcescens MG1

2006 ◽  
Vol 188 (12) ◽  
pp. 4570-4572 ◽  
Author(s):  
Rob Van Houdt ◽  
Pieter Moons ◽  
Maria Hueso Buj ◽  
Chris W. Michiels
Keyword(s):  
Quorum Sensing ◽  
Serratia Marcescens ◽  
Acid Production ◽  
Growth Arrest ◽  
Homoserine Lactone ◽  
Early Growth ◽  
Signal Molecules ◽  
Serratia Plymuthica ◽  
Synthetic Signal ◽  
Signal Production

ABSTRACT Butanediol fermentation in two Serratia species is shown to be affected by N-acyl-l-homoserine lactone-dependent quorum sensing. Knockout of quorum-sensing signal production caused a shift towards enhanced acid production, resulting in early growth arrest, which was reversible by the addition of synthetic signal molecules.

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