scholarly journals The nucleotide sequence of the Desulfovibrio gigas desulforedoxin gene indicates that the Desulfovibrio vulgaris rbo gene originated from a gene fusion event.

1990 ◽  
Vol 172 (12) ◽  
pp. 7289-7292 ◽  
Author(s):  
M J Brumlik ◽  
G Leroy ◽  
M Bruschi ◽  
G Voordouw
Keyword(s):  
Nucleotide Sequence ◽  
Gene Fusion ◽  
Desulfovibrio Vulgaris ◽  
Fusion Event ◽  
Desulfovibrio Gigas ◽  
Gene Fusion Event

scholarly journals Origin of the plant Tm-1-like gene via two independent horizontal transfer events and one gene fusion event

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Zefeng Yang ◽  
Li Liu ◽  
Huimin Fang ◽  
Pengcheng Li ◽  
Shuhui Xu ◽  
...  
Keyword(s):  
Horizontal Transfer ◽  
Gene Fusion ◽  
Fusion Event ◽  
Gene Fusion Event

scholarly journals A functionally conserved STORR gene fusion in Papaver species that diverged 16.8 million years ago

2021 ◽  
Author(s):  
Theresa Catania ◽  
Yi Li ◽  
Thilo Winzer ◽  
David Harvey ◽  
Fergus Meade ◽  
...  
Keyword(s):  
Gene Fusion ◽  
Gene Tree ◽  
Genomic Analysis ◽  
Duplication Event ◽  
Opium Poppy ◽  
Fusion Event ◽  
Genome Duplication Event ◽  
Benzylisoquinoline Alkaloid ◽  
Gene Fusion Event ◽  
Important Exception

The STORR gene fusion event is considered a key step in the evolution of benzylisoquinoline alkaloid (BIA) metabolism in opium poppy as the resulting bi-modular protein performs the isomerization of (S)- to (R)- reticuline which is required for morphinan biosynthesis. Our previous analysis of the opium poppy genome suggested the STORR gene fusion event occurred before a whole genome duplication event 7.2 million years ago. Here we use a combination of phylogenetic, transcriptomic, metabolomic, biochemical and genomic analysis to investigate the origin of the STORR gene fusion across the Papaveraceae family. The pro-morphinan/morphinan subclass of BIAs was present in a subset of 10 Papaver species including P. somniferum (opium poppy) and this correlated with the presence of the STORR gene fusion with one important exception. P. californicum does not produce morphinans but it does contain a STORR gene fusion that epimerizes (S)- to (R)- reticuline when heterologously expressed in yeast. The high similarity of the amino acid sequence linking the two modules of STORR along with phylogenetic gene tree analysis strongly suggests the gene fusion occurred only once and between 17-25 million years ago before the separation of P. californicum from the other Papaver species. We discovered that the most abundant BIA in P. californicum is (R)- glaucine, a member of the aporphine subclass of BIAs. Only the (S) isomer of this compound has previously been reported from nature. These results lead us to conclude that the function of the STORR gene fusion is not exclusive to morphinan production in the Papaveraceae.

scholarly journals Pan-genomic characterization of high-risk pediatric papillary thyroid carcinoma

2021 ◽  
Vol 28 (5) ◽  
pp. 337-351
Author(s):  
Adam Stenman ◽  
Samuel Backman ◽  
Klara Johansson ◽  
Johan O Paulsson ◽  
Peter Stålberg ◽  
...  
Keyword(s):  
High Risk ◽  
Single Case ◽  
Papillary Thyroid ◽  
Fusion Event ◽  
Term Outcome ◽  
Primary Tumors ◽  
Long Term Outcome ◽  
Regulatory Pathways ◽  
Genomic Landscape ◽  
Gene Fusion Event

Pediatric papillary thyroid carcinomas (pPTCs) are often indolent tumors with excellent long-term outcome, although subsets of cases are clinically troublesome and recur. Although it is generally thought to exhibit similar molecular aberrancies as their counterpart tumors in adults, the pan-genomic landscape of clinically aggressive pPTCs has not been previously described. In this study, five pairs of primary and synchronously metastatic pPTC from patients with high-risk phenotypes were characterized using parallel whole-genome and -transcriptome sequencing. Primary tumors and their metastatic components displayed an exceedingly low number of coding somatic mutations and gross chromosomal alterations overall, with surprisingly few shared mutational events. Two cases exhibited one established gene fusion event each (SQSTM1-NTRK3 and NCOA4-RET) in both primary and metastatic tissues, and one case each was positive for a BRAF V600E mutation and a germline truncating CHEK2 mutation, respectively. One single case was without apparent driver events and was considered as a genetic orphan. Non-coding mutations in cancer-associated regions were generally not present. By expressional analyses, fusion-driven primary and metastatic pPTC clustered separately from the mutation-driven cases and the sole genetic orphan. We conclude that pPTCs are genetically indolent tumors with exceedingly stable genomes. Several mutations found exclusively in the metastatic samples which may represent novel genetic events that drive the metastatic behavior, and the differences in mutational compositions suggest early clonal divergence between primary tumors and metastases. Moreover, an overrepresentation of mutational and expressional dysregulation of immune regulatory pathways was noted among fusion-positive pPTC metastases, suggesting that these tumors might facilitate spread through immune evasive mechanisms.

scholarly journals Detection of a Novel, and Likely Ancestral, Tn916-Like Element from a Human Saliva Metagenomic Library

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 548
Author(s):  
Liam J. Reynolds ◽  
Muna F. Anjum ◽  
Adam P. Roberts
Keyword(s):  
Resistance Genes ◽  
Metagenomic Library ◽  
Tetracycline Resistance ◽  
Human Saliva ◽  
Fusion Event ◽  
Tetracycline Resistance Genes ◽  
Antimicrobial Resistance Genes ◽  
Genes Encoding ◽  
Gene Fusion Event ◽  
Metagenomic Approach

Tn916 is a conjugative transposon (CTn) and the first reported and most well characterised of the Tn916/Tn1545 family of CTns. Tn916-like elements have a characteristic modular structure and different members of this family have been identified based on similarities and variations in these modules. In addition to carrying genes encoding proteins required for their conjugation, Tn916-like elements also carry accessory, antimicrobial resistance genes; most commonly the tetracycline resistance gene, tet(M). Our study aimed to identify and characterise tetracycline resistance genes from the human saliva metagenome using a functional metagenomic approach. We identified a tetracycline-resistant clone, TT31, the sequencing of which revealed it to encode both tet(M) and tet(L). Comparison of the TT31 sequence with the accessory, regulation, and recombination modules of other Tn916-like elements indicated that a partial Tn916-like element encoding a truncated orf9 was cloned in TT31. Analysis indicated that a previous insertion within the truncated orf9 created the full length orf9 found in most Tn916-like transposons; demonstrating that orf9 is, in fact, the result of a gene fusion event. Thus, we hypothesise that the Tn916-like element cloned in TT31 likely represents an ancestral Tn916.

scholarly journals Nucleotide sequence of 5S ribosomal ribonucleic acid from a sulfate-reducing bacterium, Desulfovibrio vulgaris.

1986 ◽  
Vol 34 (10) ◽  
pp. 4190-4194 ◽  
Author(s):  
KAZUNOBU MIURA ◽  
JUNJI KAKUCHI ◽  
ERIKO ENDO ◽  
TOHRU UEDA ◽  
KUNIHIKO KOBAYASHI ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Desulfovibrio Vulgaris ◽  
Sulfate Reducing ◽  
Ribosomal Ribonucleic Acid
Sign in / Sign up

Export Citation Format

Share Document